The effect of monosaccharide sugars and pyruvate on the differentiation and metabolism of sheep granulosa cells in vitro

Campbell, Bruce; Onions, V.; Kendall, N.R.; Guo, Li; Scaramuzzi, R. J.

doi:10.1530/rep-10-0146

Cited by 35 publications

(34 citation statements)

References 54 publications

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“…This condition typically occurs in women with a deficiency in or a mutation on one of the key enzymes required for galactose metabolism, namely galactose-1-phosphate uridyltransferase (GALT). Little is known of the patterns of expression of these enzymes in ruminants, but these toxic effects of galactose are consistent with the observation that high doses of galactose inhibit the FSH-induced differentiation of sheep granulosa cells (Campbell et al 2010b) and ovarian oestradiol and androstenedione secretion following direct ovarian infusion in sheep (Campbell et al 2010a). Despite a theoretical availability of the glycolytic pathway for galactose metabolism, we have found that of the monosaccharide tested, galactose was the least well used by ovine granulosa cells, with very little lactate being synthesised compared with glucose or fructose (Campbell et al 2010b).…”

Section: Introductionmentioning

confidence: 62%

“…Evidence of glucose transporter expression (SLC2A1 and SLC2A4) on granulosa and theca cells of sheep antral follicles (Williams et al 2001) and ovarian arteriovenous difference studies suggest that glucose is taken up by the ovary in significant amounts (Rabiee et al 1997;Scaramuzzi et al 2010). Furthermore, studies using cultured cells in our laboratory have shown that glucose, metabolised under anoxic conditions to lactate, is the preferred energy substrate to support the gonadotrophin-induced differentiation of ovine granulosa cells (Campbell et al 2010b). …”

Section: Introductionmentioning

confidence: 87%

“…These were semiquantified by real-time PCR using the 7500 Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), with samples being amplified in triplicate for determination of expression of SLC2A1, SLC2A4, SLC2A5 and SLC2A8 using primers and PCR conditions described previously (Campbell et al 2010b). Arbitrary SLC2A expression was normalised against starting amount differences using an 18s endogenous control.…”

Section: Assays Of Spent Culture Mediummentioning

confidence: 99%

“…Conversely, the evidence that fructose has a role in ovarian physiology is equivocal. Although expression of SLC2A5 has not been detected in rat ovary (Kol et al 1997) or ovine granulosa cells (Campbell et al 2010b), fructose can support the FSH-induced differentiation of cultured granulosa cells (Campbell et al 2010b). In primates, including humans, fructose does not affect leptin or insulin release or blood glucose levels (Curry 1989;Havel 1997;Teff et al 2004).…”

Section: Introductionmentioning

confidence: 98%

“…The expression of several SLC2As that transport galactose (SLCA21, SLCA22, SLCA23 and SLCA28) has been reported in the ruminant ovary (Williams et al 2001;Nishimoto et al 2006;Pisani et al 2008;Campbell et al 2010b). Furthermore, rodent and human ovaries express high levels of the enzymes that metabolise galactose via the Leloir pathway (Liu et al 2000), and accumulation of galactose or its metabolites in humans results in infertility due to impaired ovarian function.…”

Section: Introductionmentioning

confidence: 99%

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Effect of monosaccharide sugars on LH-induced differentiation and sugar transport facilitator (SLC2A) expression in sheep theca cells in vitro

Campbell

Kendall

Onions

et al. 2014

Reprod. Fertil. Dev.

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Abstract. The aim of the present study was to investigate the effects of glucose, galactose and fructose on the LH-induced differentiation and mRNA expression of sugar transport facilitators (SLC2A) by sheep thecal cells derived from small antral follicles cultured under serum-free conditions for 6 days. The dose and type of monosaccharide had a significant effect on LH-induced androstenedione production by theca cells and there was a significant interaction (P , 0.001). Glucose and galactose were used with equal efficiency so that cell numbers and androstenedione production at the end of the culture were comparable. Pharmacological doses of glucose (16.7 mM) inhibited steroidogenesis (P , 0.05). Cell numbers and androstenedione production by cells cultured with fructose were lower than for cells cultured with either glucose or galactose (P , 0.001). None of the monosaccharides resulted in the production of lactate. Expression of SLC2A1, SLC2A4 and SLC2A8, but not SLC2A5, mRNA was detected in fresh and cultured theca cells. Large doses (16.7 mM) of glucose and fructose, but not galactose, suppressed (P , 0.05) SLC2A expression. The results show that glucose and galactose, but not fructose, are readily metabolised via oxidative pathways to support LH-induced differentiation of sheep theca cells. Further work is required to determine the mechanisms resulting in these differences in relation to the established effects of nutrition on reproductive function.

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Section: Introductionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 87%

Section: Assays Of Spent Culture Mediummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Effect of monosaccharide sugars on LH-induced differentiation and sugar transport facilitator (SLC2A) expression in sheep theca cells in vitro

Campbell

Kendall

Onions

et al. 2014

Reprod. Fertil. Dev.

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Proteome profiling of chicken ovarian follicles immediately before and after cyclic recruitment

Ghanem

Johnson

2021

Molecular Reproduction Devel

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A shotgun proteomics study using isobaric tags for relative and absolute quantification labeling was conducted to characterize proteins in chicken ovarian follicles immediately before and after cyclic recruitment. Granulosa cell (GC) layers from the most recently recruited follicle (GC9) and from each of the four largest prerecruitment follicles (GC1-4) plus theca tissue (TH) from the most recently recruited (TH9) and largest prerecruitment (TH1) follicles were compared. Of 1535 proteins identified, none were determined to be differentially expressed between TH9 and TH1. A pairwise comparison between GC9 and GC1, GC2, GC3, or GC4 resulted in one, five, five, and six differentially expressed proteins, respectively, including yolk and cholesterol transport proteins (vitellogenin 1-3 and apolipoprotein B). In addition, transforming growth factor-beta 1 and microRNA-21 pathways were predicted to be activated at recruitment. We also report, for the first time, the expression of the neuropeptide, RELAXIN-3 (RLN3), in GC. Quantitative polymerase chain reaction determined RLN3 expression to be highest in GC9 and GC1, but its receptors, RXFP1 and RXFP3, were highest in TH and ovarian stroma, respectively. Overall, cyclic recruitment is associated with changes in protein expression predominantly within follicle GC, and a potential role for RLN3 in follicle recruitment and the initiation of GC differentiation warrants further investigation.

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Bovine Granulosa Cell Culture

Mohammed

Donadeu

2018

Methods in Molecular Biology

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Culture of granulosa cells has for long provided a useful tool to understand the molecular processes underlying ovarian follicle development. Among all species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we describe up-to-date methods for the harvesting of granulosa cells from bovine ovaries collected post-mortem, as well as procedures for both culturing granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro.

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The effect of monosaccharide sugars and pyruvate on the differentiation and metabolism of sheep granulosa cells in vitro

Cited by 35 publications

References 54 publications

Effect of monosaccharide sugars on LH-induced differentiation and sugar transport facilitator (SLC2A) expression in sheep theca cells in vitro

Effect of monosaccharide sugars on LH-induced differentiation and sugar transport facilitator (SLC2A) expression in sheep theca cells in vitro

Proteome profiling of chicken ovarian follicles immediately before and after cyclic recruitment

Bovine Granulosa Cell Culture

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