The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens

Biswas, Avishek; Dev, Kapil; Tyagi, Pramod K.; Mandal, A.B.

doi:10.5713/ab.20.0749

Cited by 28 publications

(17 citation statements)

References 46 publications

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“…Supplementing the diets of neonatal calves with probiotics can stimulate body growth 16,17 and organ development, 18 reduce the incidence of diarrhea, 17 and improve animal welfare and health, 19 providing long‐lasting benefits for sustainable milk production and metabolic profiling 20 . Studies in neonatal calves clearly reveal the importance of probiotic supplementation for improving the performance and health of Holstein calves 21,22 in terms of the immunity, expression of nutrient transporter genes, gut morphometry and microbiota of calves 17,23,24 . However, the effects of probiotics on small intestine development in neonatal calves have not been investigated thoroughly.…”

Section: Introductionmentioning

confidence: 99%

“… 20 Studies in neonatal calves clearly reveal the importance of probiotic supplementation for improving the performance and health of Holstein calves 21 , 22 in terms of the immunity, expression of nutrient transporter genes, gut morphometry and microbiota of calves. 17 , 23 , 24 However, the effects of probiotics on small intestine development in neonatal calves have not been investigated thoroughly.…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary supplementation with multispecies probiotics on intestinal epithelial development and growth performance of neonatal calves challenged with Escherichia coli K99

Nie

et al. 2022

J Sci Food Agric

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BACKGROUND Probiotics exhibit antibiotic properties and are capable of treating certain bacterial infections, including diarrhea. Therefore, the aim of this study is to investigate the effects of dietary supplementation with multispecies probiotic (MSP) on diarrhea, average daily gain (ADG) and intestinal development of neonatal calves challenged with Escherichia coli K99. RESULTS Thirty‐six neonatal Holstein calves were randomly assigned to three treatment groups. After E. coli K99 challenge, calves in the control (C) and MSP treatment groups had significantly higher ADG and feed efficiency, and significantly lower fecal scores than those of calves in the diarrhea (D) group. The mean time of diarrhea resolution was 4.5 and 3.1 days for calves in the D and MSP treatment groups, respectively. Furthermore, the structures of the various segments (duodenum, jejunum and ileum) of the small intestine of the calves, activities of several small intestinal enzymes, and expression of several energy metabolism‐related genes in the small intestine segments were significantly affected by MSP treatments. CONCLUSION Dietary supplementation of MSP had a positive effect in treating calf diarrhea; it improved ADG and feed efficiency and promoted development of the small intestine. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation with multispecies probiotics on intestinal epithelial development and growth performance of neonatal calves challenged with Escherichia coli K99

Nie

et al. 2022

J Sci Food Agric

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“…These results agreed with those of Pacifici et al ( 61 ), who reported that intra-amniotic administration of raffinose upregulated SGLT1 expression significantly. Similarly, dietary supplementation of multi-strain probiotics upregulated the expression of SGLT1 in broilers at 14 days post-hatch ( 81 ). The upregulated expression of brush-border membrane functional genes indicates the higher function and development of the small intestine and better nutrient absorption, leading to improving the growth performance of birds.…”

Section: Discussionmentioning

confidence: 99%

“…These supplements also contribute to eliminating the use of prophylactic drugs. The immunomodulating capabilities of these supplements are represented in several reports ( 9 , 75 , 81 ). TLR-2 is a pathogen recognition receptor expressed on infectious agents to recognize the molecular patterns associated with microbes and play a vital role in initiating and regulating the innate immune response.…”

Section: Discussionmentioning

confidence: 99%

In ovo Inoculation of Bacillus subtilis and Raffinose Affects Growth Performance, Cecal Microbiota, Volatile Fatty Acid, Ileal Morphology and Gene Expression, and Sustainability of Broiler Chickens (Gallus gallus)

et al. 2022

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Banning antibiotic growth promoters has negatively impacted poultry production and sustainability, which led to exploring efficient alternatives such as probiotics, probiotics, and synbiotics. Effect of in ovo injection of Bacillus subtilis, raffinose, and their synbiotics on growth performance, cecal microbial population and volatile fatty acid concentration, ileal histomorphology, and ileal gene expression was investigated in broilers (Gallus gallus) raised for 21 days. On 300 h of incubation, a total of 1,500 embryonated eggs were equally allotted into 10 groups. The first was non-injected (NC) and the remaining in ovo injected with sterile distilled water (PC), B. subtilis 4 × 105 and 4 × 106 CFU (BS1 and BS2), Raffinose 2 and 3 mg (R1 and R2), B. subtilis 4 × 105 CFU + raffinose 2 mg (BS1R1), B. subtilis 4 × 105 CFU + raffinose 3 mg (BS1R2), B. subtilis 4 × 106 CFU + raffinose 2 mg (BS2R1), and B. subtilis 4 × 106 CFU + raffinose 3 mg (BS2R2). At hatch, 60 chicks from each group were randomly chosen, divided into groups of 6 replicates (10 birds/replicate), and fed with a corn–soybean-based diet. In ovo inoculation of B. subtilis and raffinose alone or combinations significantly improved body weight, feed intake, and feed conversion ratio of 21-day-old broilers compared to NC. Cecal concentrations of butyric, pentanoic, propionic, and isobutyric acids were significantly elevated in R1, R2, BS2R1, and BS2R2, whereas isovaleric and acetic acids were significantly increased in R1 and BS2R1 compared to NC. Cecal microbial population was significantly altered in treated groups. Ileal villus height was increased (p < 0.001) in BS1, R2, and BS2R2 compared to NC. The mRNA expression of mucin-2 was upregulated (p < 0.05) in synbiotic groups except for BS1R1. Vascular endothelial growth factor (VEGF) expression was increased (p < 0.05) in BS2, R1, BS1R1, and BS1R2 compared to NC. SGLT-1 expression was upregulated (p < 0.05) in all treated birds except those of R1 group compared to NC. The mRNA expressions of interleukin (IL)-2 and toll-like receptor (TLR)-4 were downregulated (p < 0.05) in BS2 and R1 for IL-2 and BS1R1 and BS2R2 for TLR-4. It was concluded that in ovo B. subtilis, raffinose, and synbiotics positively affected growth performance, cecal microbiota, gut health, immune responses, and thus the sustainability of production in 21-day-old broilers.

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“…Throughout history, Saccharomyces cerevisiae has been an indispensable fungus in agriculture and the food industry [1,2]. Besides the widespread and diverse applications of this yeast in various industries, a subtype of the species, namely S. 'boulardii' is applied commonly as a probiotic both for human use and even for livestock [3][4][5][6][7]. It is by far the leading probiotic yeast in the world [8] and its probiotic properties have been demonstrated in more than 80 randomized clinical trials for the strain S. 'boulardii' CNCM I-745 [6].…”

Section: Introductionmentioning

confidence: 99%

Virulence Factors and in-Host Selection on Phenotypes in Infectious Probiotic Yeast Isolates (Saccharomyces ‘boulardii’)

Imre

Kovács

Pázmándi

et al. 2021

JoF

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Saccharomyces yeast probiotics (S. ’boulardii’) have long been applied in the treatment of several gastrointestinal conditions. Despite their widespread use, they are rare opportunistic pathogens responsible for a high proportion of Saccharomyces mycosis cases. The potential virulence attributes of S. ’boulardii’ as well as its interactions with the human immune system have been studied, however, no information is available on how these yeasts may change due to in-host evolution. To fill this gap, we compared the general phenotypic characteristics, cell morphology, virulence factors, epithelial and immunological interactions, and pathogenicity of four probiotic product samples, two mycosis, and eight non-mycosis samples of S. ’boulardii’. We assessed the characteristics related to major steps of yeast infections. Mycosis and non-mycosis isolates both displayed novel characters when compared to the product isolates, but in the case of most virulence factors and in pathogenicity, differences were negligible or, surprisingly, the yeasts from products showed elevated levels. No isolates inflicted considerable damage to the epithelial model or bore the hallmarks of immune evasion. Our results show that strains in probiotic products possess characteristics that enable them to act as pathogens upon permissive conditions, and their entry into the bloodstream is not due to active mechanisms but depends on the host. Survival in the host is dependent on yeast phenotypic characteristics which may change in many ways once they start evolving in the host. These facts call attention to the shortcomings of virulence phenotyping in yeast research, and the need for a more thorough assessment of probiotic use.

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The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens

Abstract: The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens

Cited by 28 publications

References 46 publications

Effects of dietary supplementation with multispecies probiotics on intestinal epithelial development and growth performance of neonatal calves challenged with Escherichia coli K99

Effects of dietary supplementation with multispecies probiotics on intestinal epithelial development and growth performance of neonatal calves challenged with Escherichia coli K99

In ovo Inoculation of Bacillus subtilis and Raffinose Affects Growth Performance, Cecal Microbiota, Volatile Fatty Acid, Ileal Morphology and Gene Expression, and Sustainability of Broiler Chickens (Gallus gallus)

Virulence Factors and in-Host Selection on Phenotypes in Infectious Probiotic Yeast Isolates (Saccharomyces ‘boulardii’)

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