The Effect of Mycoplasmas on Replication and Plaquing Ability of Herpes Simplex Virus

Manischewitz, J E; Young, Bobby G.; Barile, Michael F.

doi:10.3181/00379727-148-38648

Cited by 12 publications

(3 citation statements)

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“…The mycoplasma problem is dealt with by investigators in a variety of ways: many simply ignore the mycoplasma and hope that it will not affect results, whereas others actively seek to ensure that no contaminated cells or virus are used in experiments. Mycoplasma contamination may reduce herpesvirus production (11) and, at the levels described in this presentation, may induce a contaminating DNA polymerase activity in cell extracts. Since the mycoplasma polymerase was found to be stimulated by salt, determining the effect of various inhibitors on salt-stimulated polymerase activity of extracts of herpesvirus-infected cells could easily be misinterpreted.…”

Section: Discussionmentioning

confidence: 89%

Distinguishing cytomegalovirus, mycoplasma, and cellular DNA polymerases

Miller¹,

Rapp²

1976

J Virol

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Cytomegalovirus-induced DNA polymerase can be distinguished from infected-cell enzymes by activity in 100 mM (NH4),SO4. Virus polymerase is stimulated to 145% of control, whereas mock-infected cell polymerase is inhibited to 12% of control without added salt. Mycoplasmas induce a DNA polymerase in cell extracts that is stimulated to 130 to 180% by 25 mM (NH4)2SO4. Mycoplasma DNA polymerase may be mistaken for a virus-induced polymerase when virus stocks are contaminated. Identification of virus, cellular, and mycoplasma DNA polymerases in total cell extracts is described using sedimentation rate and effect of inhibitors on DNA polymerase activities.

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Section: Discussionmentioning

confidence: 89%

Distinguishing cytomegalovirus, mycoplasma, and cellular DNA polymerases

Miller¹,

Rapp²

1976

J Virol

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“…arginini decreases the yield of HSV 1 and reversion is obtained by supplementing the medium with arginine while Acholeplasma laidlawii, a non-arginine utilizer, has no effect [67]. It has been also shown that contamination of chick embryo cells by M. gallisepticum markedly affects the in vitro multiplication of fowlpox virus and modifies the plaque morphology; this mycoplasma also decreases the in vivo infectivity of fowlpox virus for day-old chicks [31].…”

Section: Ycoplasmas As Frequent Contaminants Of Cell Cultures: Intementioning

confidence: 94%

Links and interactions between mycoplasmas and viruses: past confusions and present realities

Chastel¹

1995

Archives of Virology

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Links between mycoplasmas and viruses are ancient, multiple and complex, from past confusions during the first decades of the virus era to present realities illustrated by the possible implication of mycoplasmas as co-factors in natural infections of AIDS. Mycoplasma viruses (phages) may also be responsible for modifying the pathogenic power of mycoplasmas, at least for plants and insects. In addition, several mycoplasmas are able to act as undesirable cell culture contaminants that induce erroneous results in both applied and general virology. These problems are examined within a historical context.

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“…Mycoplasmas may alter the progress of viral infections in cell cultures [83,84]. As mycoplasmas may also cause virus-like CPE, many investigators have mistaken cytolytic mycoplasmas for viruses.…”

Section: Effect On Virus Infectionmentioning

confidence: 99%