The effect of Na+ and osmolality on the influx and steady state distribution of taurine and gamma-aminobutyric acid in flounder (Platichthys flesus) erythrocytes

Fugelli, K.; Rohrs, Harold C.

doi:10.1016/0300-9629(80)90239-x

Cited by 31 publications

(27 citation statements)

References 17 publications

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“…It is most likely that it is the resulting changes in cell volume which are responsible for these changes. Fugelli & Rohrs (1980) have presented similar results regarding taurine distribution in flounder erythrocytes.…”

Section: Discussionsupporting

confidence: 73%

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

Hoffmann¹,

Lambert²

1983

The Journal of Physiology

128

102

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SUMMARY1. Cellular and extracellular concentrations of amino acids were measured in Ehrlich ascites cells by amino acid analysis and by distribution of radioactive amino acids between cells and medium. Dilution of the medium results in a reduction in the cellular concentration of non-essential amino acids and taurine and an equivalent increase in the extracellular content of these amino acids.2. The membrane potential and the electrochemical gradient of sodium were measured. The decrease in the cellular to extracellular gradient of taurine and glycine is not a consequence of a decrease in the sodium gradient but is caused by the changes in osmolarity and cell volume.3. The unidirectional influx and efflux of glycine and taurine were measured as the initial flux of [14C]glycine and [35S]taurine, using a rapid filter technique. For both amino acids the rate constants for influx are decreased and the rate constants for efflux increased following a reduction in osmolarity.4. The taurine and glycine fluxes were analysed as a simple pump and leak system. Reduction in osmolarity increases the leak permeability to both taurine and glycine.5. The results for glycine are also discussed in relation to a Na+-glycine co-transport model, where reduction in osmolarity increases the leak permeability to glycine.6. The cellular permeability to taurine and glycine increases as a function of increasing cell volume. The taurine permeability increases 7-fold and the glycine permeability 1-5-fold with an increase in cell volume of 30 %. The absolute increase in permeability is equal for both taurine and glycine.

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Section: Discussionsupporting

confidence: 73%

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

Hoffmann¹,

Lambert²

1983

The Journal of Physiology

128

102

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“…Blood was obtained by puncturing the bulbus arteriosus using a heparinized syringe and then transferred to incubation syringes (Fugelli & Rohrs, 1980). The blood cells were isolated by centrifugation, and plasma and buffy coat were removed by aspiration.…”

Section: Ta Urine Transport In Flo Under Er Ythroc Ytes Experimental mentioning

confidence: 99%

“…The cells in the incubation syringes were equilibrated in their respective media in a temperaturecontrolled water bath (10-0+01 0C) according to Fugelli & Rohrs (1980). Cell volume.…”

Section: Ta Urine Transport In Flo Under Er Ythroc Ytes Experimental mentioning

confidence: 99%

“…Taurine is the dominating amino compound osmo-effector in flounder erythrocytes (Fugelli & Zachariassen, 1976;Fugelli & Rohrs, 1980). An osmolality reduction induces a net release of this solute by an apparently controlled process.…”

Section: Introductionmentioning

confidence: 99%

“…An osmolality reduction induces a net release of this solute by an apparently controlled process. This is suggested by the linear correlation between the cellular taurine content and the plasma osmolality (Fugelli & Zachariassen, 1976;Fugelli & Rohrs, 1980). However, the taurine transport systems that are engaged in the r.v.d.…”

Section: Introductionmentioning

confidence: 99%

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Taurine transport associated with cell volume regulation in flounder erythrocytes under anisosmotic conditions.

Fugelli¹,

Thoroed²

1986

The Journal of Physiology

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SUMMARY1. The taurine transport of flounder erythrocytes is associated with a cell volume regulation in anisosmotic media. An osmolality reduction leads to a cell volume increase, which is followed by a volume readjustment towards the original level. A 75 mosm reduction is accompanied by a 33 ,mol g dry wt.-' reduction in the cellular taurine content.2. The reduction in osmolality activates the taurine release mechanism by transiently increasing the rate coefficient for taurine efflux. The rate coefficient for taurine influx is similarly stimulated. This influx is mediated by a Na+-independent transport system. The concomitant activation of influx and efflux suggests a coupling between these two systems.3. Higher taurine efflux and influx rate coefficients which decayed more slowly with time were measured in cells suspended in Na+-free (choline replacement) media than in the presence of Na+. This suggests that Na+ may play a role in the taurine release mechanism.4. Noradrenaline induced a cellular swelling at normal osmolality (330 mosm), but had only a minor effect on the taurine efflux and influx and the cellular taurine content.5. Urea-induced cellular swelling at normal osmolality initiated a volume regulatory process and activated the taurine release mechanism, similarly to an osmolality reduction.6. These results show that osmolality reduction and cellular swelling are no prerequisites for the activation of the taurine release mechanism and the cell volume readjustment.7. It is suggested that the dimension of an intracellular solute compartment determines the activation level of this mechanism.* The order of names is alphabetical.

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Time‐course changes in the regulation of ions and amino acids in the hard clam Meretrix lusoria upon lower salinity challenge

2021

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In this study, we examined ion and amino acid regulation in the gill and mantle of the hard clam Meretrix lusoria. We found that the osmolality and Na + and Clconcentrations of hard clam hemolymph were significantly reduced after transferring clams from the salinity of their natural habitat [20‰ saltwater (SW)] to a lower salinity environment (10‰ SW). Specific activities of Na + , K + -ATPase (NKA), which provides the driving force for the secondary ion transport associated with cell osmoregulation in gills and mantles, were unaffected during the acclimation to lower salinity. In contrast, there was a significant decline in the contents of free amino acids (FAAs) in the gills and mantles of hard clams during lower salinity acclimation.

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The effect of Na+ and osmolality on the influx and steady state distribution of taurine and gamma-aminobutyric acid in flounder (Platichthys flesus) erythrocytes

Cited by 31 publications

References 17 publications

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

Taurine transport associated with cell volume regulation in flounder erythrocytes under anisosmotic conditions.

Time‐course changes in the regulation of ions and amino acids in the hard clam Meretrix lusoria upon lower salinity challenge

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