The effect of NaCl on growth and volatile metabolites produced by antagonistic endophytic bacteria isolated from Prosopis cineraria

Al-Rashdi, Ahad; Al-Sadi, Abdullah M.; Al-Harrasi, Majida Mohammed Ali; Al-Sabahi, Jamal Nasser; Janke, Rhonda R.; Velazhahan, R.

doi:10.1007/s13313-023-00922-y

Cited by 2 publications

(1 citation statement)

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“…The VOCs released by each bacterium were collected separately, according to Jayakumar et al [ 42 ]. The collected VOCs were then analyzed by GC-MS using a Shimadzu GC-2010 Plus Gas Chromatograph (Shimadzu Corporation, Kyoto, Japan) equipped with a GCMS-QP2010 ULTRA MS and Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 μm), as described by Al-Rashdi et al [ 43 ]. The NIST 2011 v.2.3 and Wiley 9th edition mass spectral libraries were used for compound identification.…”

Section: Methodsmentioning

confidence: 99%

Rice Weevil (Sitophilus oryzae L.) Gut Bacteria Inhibit Growth of Aspergillus flavus and Degrade Aflatoxin B1

Al-Saadi,

Al-Sadi,

Al-Wahaibi

et al. 2024

JoF

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In this study, bacteria residing in the gut of the rice weevils (Sitophilus oryzae L.) (Coleoptera: Curculionidae) feeding on aflatoxin-contaminated corn kernels were isolated and evaluated for their ability to suppress Aspergillus flavus and to remove/degrade aflatoxin B1 (AFB1). Four morphologically distinct S. oryzae gut-associated bacterial isolates were isolated and identified as Bacillus subtilis (RWGB1), Bacillus oceanisediminis (RWGB2), Bacillus firmus (RWGB3), and Pseudomonas aeruginosa (RWGB4) based on 16S rRNA gene sequence analysis. These bacterial isolates inhibited A. flavus growth in the dual culture assay and induced morphological deformities in the fungal hyphae, as confirmed by scanning electron microscopy. All four bacterial isolates were capable of removing AFB1 from the nutrient broth medium. In addition, culture supernatants of these bacterial isolates degraded AFB1, and the degradation of toxin molecules was confirmed by liquid chromatography-mass spectrometry. The bacterial isolates, B. subtilis RWGB1, B. oceanisediminis RWGB2, and P. aeruginosa RWGB4, were capable of producing antifungal volatile organic compounds that inhibited A. flavus growth. These results suggest that the bacterial isolates from S. oryzae gut have the potential to bind and/or degrade AFB1. Further research on their application in the food and feed industries could enhance the safety of food and feed production.

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Section: Methodsmentioning

confidence: 99%