The effect of nanoencapsulation of ICG on two-photon bioimaging

Kumari, Anshu; Kumari, Kalpana; Gupta, Sharad

doi:10.1039/c9ra03152a

Cited by 15 publications

(11 citation statements)

References 51 publications

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“…To enable adequate tracking of the photothermal agents in tumor cells, cell nuclei were stained with Hoechst 33 256 which is imbued with a blue fluorescence. Consequently, the red and green fluorescence of ICG@GC-PP and GC-PP-FI NPs, respectively, could be clearly visualized in the cell membranes and nuclei of KYSE-30 ( Figure 5) and 4T1 ( Figure S11 [46] and HeLa, [47] free ICG also accumulated mostly in cell membranes and showed weak fluorescence signal. In 4T1 cell membranes however, free ICG provides a considerably bright fluorescence compared with those of ICG@GC-PP NPs.…”

Section: Resultsmentioning

confidence: 99%

“…By comparison, the red fluorescence of free ICG is quite weak in KYSE‐30 cells and mainly domiciled in the cell membranes of both 4T1 and KYSE‐30 cells. In other cancer cell lines such as HCT‐116 and HT‐29, [ 46 ] and HeLa, [ 47 ] free ICG also accumulated mostly in cell membranes and showed weak fluorescence signal. In 4T1 cell membranes however, free ICG provides a considerably bright fluorescence compared with those of ICG@GC‐PP NPs.…”

Section: Resultsmentioning

confidence: 99%

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A Hybrid Organo‐Nanotheranostic Platform of Superlative Biocompatibility for Near‐Infrared‐Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors

Akakuru

LIU

Iqbal

et al. 2020

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The quest for an all‐organic nanosystem with negligible cytotoxicity and remarkable in vivo tumor theranostic capability is inescapably unending. Hitherto, the landscape of available photothermal agents is dominated by metal‐based nanoparticles (NPs) with attendant in vivo negatives. Here, an all‐organic‐composed theranostic nanosystem with outstanding biocompatibility for fluorescence image‐guided tumor photothermal therapy, and as a potential alternative to metal‐based photothermal agents is developed. This is rationally achieved by compartmentalizing indocyanine green (ICG) in glycol chitosan (GC)‐polypyrrole (PP) nanocarrier to form hybrid ICG@GC‐PP NPs (≈65 nm). The compartmentalization strategy, alongside the high photothermal conversion ability of PP jointly enhances the low photostability of free ICG. Advantageously, ICG@GC‐PP is endowed with an impeccable in vivo performance by the well‐known biocompatibility track records of its individual tri organo‐components (GC, PP, and ICG). As a proof of concept, ICG@GC‐PP NPs enables a sufficiently prolonged tumor diagnosis by fluorescence imaging up to 20 h post‐injection. Furthermore, owing to the complementary heating performances of PP and ICG, ICG@GC‐PP NPs‐treated mice by one‐time near‐infrared irradiation exhibit total tumor regression within 14 days post‐treatment. Therefore, leveraging the underlying benefits of this study will help to guide the development of new all‐organic biocompatible systems in synergism, for safer tumor theranostics.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A Hybrid Organo‐Nanotheranostic Platform of Superlative Biocompatibility for Near‐Infrared‐Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors

Akakuru

LIU

Iqbal

et al. 2020

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“…Near-infrared fluorescence (NIRF) has the potential for noninvasive deep tissue imaging, particularly for early-stage cancer diagnosis. − NIR imaging is capable of higher tissue penetration and better visualization of cellular microstructure due to reduced photon scattering and negligible tissue autofluorescence in the optical window (650 to 950 nm). − Additionally, the use of NIR-active exogenous contrast agents could further improve the signal-to-noise ratio (SNR) of fluorescence imaging. , ICG is the only Food and Drug Administration (FDA)-approved cyanine dye and has been actively used for the past six decades for NIR imaging. − However, the free form of ICG has several limitations such as concentration-dependent aggregation, low thermal and optical stability, lack of targeting ability, and short circulation time in the body . Due to these limitations, the broader application of ICG as a potential contrast agent for bioimaging is not achieved yet.…”

Section: Introductionmentioning

confidence: 99%

“…39−41 Additionally, the use of NIR-active exogenous contrast agents could further improve the signal-to-noise ratio (SNR) of fluorescence imaging. 42,43 ICG is the only Food and Drug Administration (FDA)-approved cyanine dye and has been actively used for the past six decades for NIR imaging. 44−46 However, the free form of ICG has several limitations such as concentration-dependent aggregation, low thermal and optical stability, lack of targeting ability, and short circulation time in the body.…”

Section: ■ Introductionmentioning

confidence: 99%

Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging

Bishnoi

Kumari

Rehman

et al. 2021

ACS Biomater. Sci. Eng.

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An effective drug delivery system (DDS) relies on an efficient cellular uptake and faster intracellular delivery of theranostic agents, bypassing the endosomal mediated degradation of the payload. The use of viral nanoparticles (VNPs) permits such advancement, as the viruses are naturally evolved to infiltrate the host cells to deliver their genetic material. As a proof of concept, we bioengineered the vesicular stomatitis virus glycoprotein (VSV-G)-based near-infrared (NIR) active viral nanoconstructs (NAVNs) encapsulating indocyanine green dye (ICG) for NIR bioimaging. NAVNs are spherical in size and have the intrinsic cellular-fusogenic properties of VSV-G. Further, the NIR imaging displaying higher fluorescence intensity in NAVNs treated cells suggests enhanced cellular uptake and delivery of ICG by NAVNs compared to the free form of ICG. The overall study highlights the effectiveness of VSV-G-based VNPs as an efficient delivery system for NIR fluorescence imaging.

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“…For the proof of concept, indocyanine green (ICG) was encapsulated within PLL NPs. ICG is a US Food and Drug Administration (FDA) approved near-infrared (NIR) fluorescent dye used for various biomedicalapplications 46–49 . Despite its several applications, ICG has not been used to the fullest for deep tissue imaging in its free-form.…”

Section: Introductionmentioning

confidence: 99%

Protease Responsive Essential Amino-Acid Based Nanocarriers for Near-Infrared Imaging

Kumari

Gupta

2019

Sci Rep

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Delivery of the theranostic agents with effective concentration to the desired sites inside the body is a major challenge in disease management. Nanotechnology has gained attention for the delivery of theranostic agents to the targeted location. The use of essential amino-acid based homopolymers for the synthesis of biocompatible and biodegradable nanoparticles (NPs) could serve as a nanocarrier for delivery applications. In this study, poly-l-lysine (PLL) and salts were used to fabricate the NPs for the delivery of exogenous contrast agents. Here, indocyanine green (ICG) was encapsulated within these NPs, and a simple two-step green chemistry-based self-assembly process was used for the fabrication. The morphological and biochemical characterizations confirm the formation of ICG encapsulating spherical PLL NPs with an average diameter of ~225 nm. Further, a detailed study has been carried out to understand the role of constituents in the assembly mechanism of PLL NPs. Our results show a controlled release of the ICG from PLL NPs in the presence of the proteolytic enzyme. In-vitro cellular studies suggest that the PLL NPs were readily taken up by the cells showing their superior delivery efficiency of ICG in comparison to the free-form of the ICG.

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The effect of nanoencapsulation of ICG on two-photon bioimaging

Abstract: ICG-PLA NPs were synthesized for multiphoton bioimaging. The ICG-PLA NPs were more efficiently taken up by the cells and improved the photostability of the ICG. The ICG-PLA NPs incubated cells display superior contrast in multiphoton imaging.

Cited by 15 publications

References 51 publications

A Hybrid Organo‐Nanotheranostic Platform of Superlative Biocompatibility for Near‐Infrared‐Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors

A Hybrid Organo‐Nanotheranostic Platform of Superlative Biocompatibility for Near‐Infrared‐Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors

Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging

Protease Responsive Essential Amino-Acid Based Nanocarriers for Near-Infrared Imaging

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