The effect of natural dicarbonyls on activity of antioxidant enzymes in vitro and in vivo

Ланкин, В. З.; Konovalova, G. G.; Tikhaze, A. K.; Недосугова, Л. В.

doi:10.1134/s1990750812010106

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The amount of enzyme converting 1 lmol GSH per minute was taken as 1 activity unit. MDA was estimated by the standard method in the reaction with 2-thiobarbituric acid in acidic media by a formation of trimetin complex having an absorption maximum at 532 nm [31].…”

Section: Preparation Of Tissue Homogenatementioning

confidence: 99%

Apelin-12 and its structural analog enhance antioxidant defense in experimental myocardial ischemia and reperfusion

et al. 2014

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This study investigated the effects of peptide apelin-12 (H-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe-OH, A12) and its novel structural analog (H-(N(α)Me)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH, AI) on myocardial antioxidant enzyme activities, lipid peroxidation, and reactive oxygen species formation in ex vivo and in vivo models of myocardial ischemia/reperfusion (I/R) injury. Isolated working rat hearts were subjected to global ischemia and reperfusion. Infusion of 140 μM A12 or AI before global ischemia improved cardiac function recovery; increased the activity of Cu,Zn superoxide dismutase (Cu,Zn SOD), catalase (CAT), and glutathione peroxidase (GSH-Px); decreased malondialdehyde (MDA) content in reperfused heart; and reduced the formation of hydroxyl radical adduct of the spin trap 5,5-dimethyl-1-pyrroline-N-oxide in the myocardial effluent during early reperfusion compared with these indices in control. Anesthetized open-chest rats were subjected to the left anterior descending coronary artery occlusion and coronary reperfusion. Peptide A12 or its analog AI was injected intravenously at the onset of reperfusion at a dose of 0.35 μmol/kg. Treatment with A12 or AI significantly limited infarct size and reduced the activity of lactate dehydrogenase and creatine kinase MB isoenzyme in blood plasma at the end of reperfusion compared with control. These effects were accompanied by complete recovery of Cu,Zn SOD, CAT, and GSH-Px activities; and decrease in MDA content in the area at risk by the end of reperfusion. The study concluded that C-terminal fragment of native peptide apelin-12 and its synthesized analog is involved in the upregulation of cardiac antioxidant defense systems and attenuation of lipid peroxidation in myocardial I/R injury.

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Section: Preparation Of Tissue Homogenatementioning

confidence: 99%

Apelin-12 and its structural analog enhance antioxidant defense in experimental myocardial ischemia and reperfusion

et al. 2014

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“…TBARS was estimated by the standard method in the reaction with 2-thiobarbituric acid in acidic media by a formation of trimetin complex having an absorption maximum at 532 nm. 33 The measurements were done using a Shimadzu UV-2600 spectrophotometer (Japan). Protein in the supernatants was estimated by the method of Lowry.…”

Section: Determination Of Antioxidant Enzymes Activity and Tbarsmentioning

confidence: 99%

Galanin receptors activation modulates myocardial metabolic and antioxidant responses to ischaemia/reperfusion stress

Студнева

Serebryakova

Veselova

et al. 2019

Clin Exp Pharma Physio

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The neuropeptide galanin comprising 29 amino acid residues (30 in humans) is widely expressed in the central nervous system and peripheral organs and tissues. Galanin exerts its biological activities through three different G protein-coupled receptors, GalR1, GalR2 and GalR3, all of which are found in the heart. 1 Over the past decade, a wealth of data has been accumulated that substantiates targeting galanin receptors for the treatment of various human diseases and pathological conditions, including Alzheimer's disease, metabolic diseases, mood disorders, pain, anxiety and solid tumors. 2 However, the physiological and pharmacological effects of galanin in heart diseases are much less studied. We have recently demonstrated that N-terminal fragments of galanin (2-11) and(2-15) and their chemically modified analogues reduce experimental myocardial I/R injury. 3-5 These peptides increased cell viability, AbstractThe mechanisms of protective action of the neuropeptide galanin and its N-terminal fragments against myocardial ischaemia/reperfusion (I/R) injury remain obscure. The aim of this work was to study effects of a novel peptide agonist of galanin receptors [βAla14, His15]-galanin (2-15) (G1) and the full-length galanin (G2) on energy and antioxidant status of the heart with acute infarction. The peptides were synthesized by the automatic solid phase method using Fmoc technology. Their structure was identified by 1 H-NMR spectroscopy and MALDI-TOF mass spectrometry. Experiments were performed on anaesthetized open-chest rats subjected to myocardial regional ischaemia and reperfusion. Intravenous (iv) administration of optimal doses of peptides G1 and G2 (1.0 and 0.5 mg/kg, respectively, at the onset of reperfusion significantly reduced infarct size (on average by 40% compared with control) and the plasma activity of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH).These effects were associated with augmented preservation of aerobic energy metabolism, increased activity of Cu,Zn superoxide dismutase (Cu,Zn-SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and decreased lipid peroxidation in the area at risk (AAR) at the end of reperfusion. Peptide G1 showed more efficient recovery of the majority of metabolic and antioxidant parameters. The results provide evidence that the galaninergic system can be considered a promising target to reduce energy dysregulation and oxidative damage in myocardial I/R injury. K E Y W O R D Santioxidant defence, energy metabolism, galanin, myocardial infarction, necrosis markers, rat

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Glycation, antiglycation, and deglycation: Their role in aging mechanisms and geroprotective effects (literature review)

Emelyanov

2017

Adv Gerontol

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The effect of natural dicarbonyls on activity of antioxidant enzymes in vitro and in vivo

Cited by 4 publications

References 31 publications

Apelin-12 and its structural analog enhance antioxidant defense in experimental myocardial ischemia and reperfusion

Apelin-12 and its structural analog enhance antioxidant defense in experimental myocardial ischemia and reperfusion

Galanin receptors activation modulates myocardial metabolic and antioxidant responses to ischaemia/reperfusion stress

Glycation, antiglycation, and deglycation: Their role in aging mechanisms and geroprotective effects (literature review)

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