The Effect of Necrostatin -1 and Enoxaparin Molecules on Random Pattern Flap Viability

Abstract

ABSTRACT
 Distal flap necrosis is seen more often in random pattern flaps, and is a important complication that shortens the flap length. There has been much research many drugs and molecules in an effort to prevent this complication. The aim of this study was to investigate the efficacy of necrostatin-1 and enoxaparin molecules in preventing distal flap necrosis and increasing flap viability in a random pattern flap model created in rats. A total of 32 Wistar albino female rats, each weighing 300-350gr, were separated into 4 groups. All the animals underwent an operation to create a 3x9 cm caudal-based Mcfarlane flap. The treatments defined for each group were applied. Full layer tisssue samples 1x1 cm2 were taken from all the flaps and stored until histopathological and immunohistochemical examination, The parameters of inflammation, capillary proliferation, necrosis, fibroblast proliferation and fibrosis were compared histopathologically. In the necrostatin-1 group, the inflammation, necrosis and fibrosis scores were observed to be lower, and the capillary proliferation and fibroblast proliferation scores were higher. In the enoxaparin group, the fibroblast proliferation and capillary proliferation scores were higher. The receptor interacting protein kinase-1 immunohistochemical staining results showed statistically significantly less staining in the necrostatin-1 group compared to the other groups. The results of this study suggest that necrostatin molecule has important therapeutic potential in increasing flap viability in the random pattern flap model, considering the percentage of flap necrosis, and the immunohistochemical and histopathological data. The flap necrosis percentage and histochemical parameters of the enoxaparin molecule demonstrate that the effects on flap viability are limited.