The Effect of Neuraminidase on the Fate of Transfused Lymphocytes

Abstract

Evidence has been obtained that incubation of rat lymphocytes with neuraminidase, prior to intravenous transfusion into allogeneic or syngeneic recipients, alters the distribution of the cells. Many enzyme-treated lymphocytes initially become trapped in the liver, and there is a decrease in the selective accumulation of these cells in the lymph nodes and spleen. Subsequently, many enzyme-altered cells emigrate from the liver, concentrate in lymph nodes, and recirculate to the lymph. The results suggest that sialic acid constituents of the lymphocyte surface play a critical role in ensuring the normal distribution of these cells in the body. The findings also imply that reactions involving surface sialic acid can markedly alter the fate of lymphocytes without "killing" the cells.

Vitamin A Deficiency and Immunity.

“…It has been chemically proven that these glycoproteins cover T-and B-lymphocyte membranes [120]. Lymphocyte treatment with a glycosidase, trypsin or neuraminidase before intravenous transfusion significantly alters the normal mode of circulation of these cells [117][118][119]. In rats, for example, the redistribution of some lymphocyte subpopulations could deplete splenic lymphocytes which respond to ConA, PHA or LPS and reveal the existence of a suppressor T lymphocyte population responsible for the inhibited blastogenic response [51].…”

“…These proteins are constituents of cell membranes and participate in several functions that may be altered during vitamin A deficiency [69,[113][114][115][116]. The hypothesis for the mode of action of vitamin A is based on this observation: glycoproteins represent important cellular receptors for numerous biological effectors, in particular mitogens and recognition molecules that determine lymphocyte distribution in lymph nodes and spleen [117][118][119]. It has been chemically proven that these glycoproteins cover T-and B-lymphocyte membranes [120].…”

A New Method for Tolerance Induction: Busulfan Administration Followed by Intravenous Injection of Neuraminidase‐Treated Donor Bone Marrow

“…To address these problems, we employed the i.v. injection of Neu-treated BMCs, since it has been reported that Neu-treated hematopoietic cells lose terminal sialic acid residues of ascialoglycoprotein on their surface and exhibit an enhanced affinity for the liver [31][32][33][34]. Moreover, it has been reported that the i.v.…”

Incidence of Nasopharyngeal Carcinoma in Malaysia, with Special Reference to the State of Selangor

“…The polyanion d-sulphate which accelerated leukaemia development in AKR mice is known to inhibit in vitro immune cytolysis (Ebbesen, 1972). Also, cellular immune reaction could be influenced by polycation induced alteration in membrane change since others have found that polyeation treatment (Larson and Olsen, 1968;Nordling, Anderson and Hayiy, 1972) and neuraminidase treatment (Woodruff and Gesner, 1969), which decrease the outer negative membrane charge will facilitate lodging of leukaemic cells in organs destroying leucocytes. A depletion of potentially malignant T lymphocytes and/or malignant T lymphocytes due to enhancement of humoral and/or cellular immune reactions therefore seems a likely explanation of the life prolonging effect of polycation treatment of the AKR mice.…”