The effect of regeneration of Lolium perenne and Festuca rubra from embryogenic suspension cells and protoplasts on fertility and growth was evaluated. Embryogenic suspension cultures were either routinely subcultured or cryopreserved and re-established. Phenology, morphology and fertility of regenerated plants were studied for two growing seasons in a replicated field experiment. Most regenerated L. perenne and F. rubra plants showed a delay in inflorescence emergence, a reduced seed yield and differences in morphological traits when compared with seed-grown plants. For L. perenne, performance of plants regenerated from cryopreserved suspension cultures and protoplasts was similar to that of respective plants regenerated from routinely maintained suspension cultures. However, differences in performance were observed for respective regenerants in F. rubra. The phenotypic deviation observed was partly reflected in the randomly amplified polymorphic DNA (RAPD) analysis performed. However, regenerants of both species showing similar, or even superior performance to the seed-grown plants were also found. Embryogenic suspension cells and corresponding protoplasts of L. perenne and F. rubra have the potential for producing fertile, well-performing plants which can be integrated in breeding programs.Lolium perenne L. and Festuca rubra L. are two key forage and turf grasses in temperate regions throughout the world. L. perenne is probably the most important forage grass of intensive managed grassland, and F. rubra is an important species of higher altitudes and extensively managed grassland. Both species are also used to a considerable extent for sports fields, lawns and golf courses.Forage and amenity grass breeding are required to respond rapidly and precisely to changing demands and to increase efficiency and minimize costs. Biotechnology has the potential to complement and speed up conventional breeding (van Heeswijck et al. 1994, Spangenberg et al. 1995. A basic prerequisite for the use of many biotechnological approaches is the efficient regeneration of true-to-type plants from embryogenic cell suspensions or protoplasts of agronomically important germplasm. Embryogenic suspension cells can be used as targets for biolistic^"^ (Bio-Rad Laboratories, Inc.) transformation (Wang et al. 1994). They are also a unique source of totipotent protoplasts for graminaceous monocot species. Furthermore, protoplasts are valuable tools for somatic hybridization via protoplast fusion and for the recovery of transgenic plants by direct gene transfer (Kaul 1990, Potrykus 1990. Maintenance of embryogenic cell suspensions by routine subculture is timeconsuming and runs the risk of contamination and genetic changes that may lead to the loss of valuable material (Wang et al. 1994). In addition, suspension cultures lose their morphogenic potential during prolonged culture (Kasperbauer 1990, Olesen et al. 1996. To overcome some of these serious limitations, cryopreservation technology has been successfully used as a tool for the long-term ...