The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

Abstract

This study aimed to assess the level of testicular damage by observing the changes in the diameter and epithelium thickness of seminiferous tubules in rats that exposure to nicotine per inhalation. Thirty adult male rats were used and divided into five equal groups and treatment as follows for 20 days; Control group NaCl 0.9%, P1 nicotine 0.5 mg/kg, P2 nicotine 1.0 mg/kg, P3 nicotine 2.0 mg/kg and P4 nicotine 4.0 mg/kg. All groups were given treatment per inhalation for twenty days. At the end of treatment and the rats were sacrificed testes were collected for histopathological preparation. The testes were processed for routine paraffin embedding and staining and the sections were examined for histopathological changes. There results showed that nicotine administration induced varying degrees of structural damage to the seminiferous tubules, as the decreased in diamater and epithelium thickness of seminiferous tubules. The diameter and epithelium thickness of seminiferous tubules in four experimental groups reduced compared to the control group. This study proves that nicotine administration does decreases the spermatogenesis of rats by reducing the diameter and epithelium thickness of seminiferous tubules in testes. It also proves that the level of testicular damage is directly proportional to the dosage of nicotine administrated to male rats.

Impact of Electronic Cigarette Exposure on the Testes of Adult Male Albino Rat and the Role of Cessation: Histopathological and Biochemical study

“…This was in line with the findings of Mohamed and Abdelrahman [73], who found a substantial reduction in the diameter and surface area of the seminiferous tubule in the nicotinetreated group compared to the control group. On the other hand, En et al [74] exposed rats to different concentrations of nicotine (0.5 mg/kg, 1.0 mg/kg, 2.0 mg/kg, and 4.0 mg/kg) and stated that when compared to the control, the mean diameter of seminiferous tubules that got a nicotine treatment of 4.0 mg/kg exhibited a substantial decrease while treatment groups such as 0.5 mg/kg, 1.0 mg/kg, and 2.0 mg/kg demonstrated a negligible decrease. Based on certain investigations, the death of germinal cells causes the diameter of seminiferous tubules to decrease, resulting in a decrease in the epithelial thickness of seminiferous tubules.…”

The influence of ethanol extract of dayak onion (Eleutherine palmifolia) on the number of Leydig cells and seminiferous tubules diameter in mice (Mus musculus) exposed to monosodium glutamate

“…Increased Ca 2+ due to NMDAR receptor activity can also activate calpains and cathepsins as proteolytic systems in cell death [7]. A decrease in Leydig cell numbers due to brain cell death will affect testosterone production, thus impacting spermatogenesis and the number of spermatogenic cells [24]. The decrease in Leydig cell numbers in the positive control group C (+) leads to narrowing of the interstitial tissue and closer spacing between seminiferous tubules (Figure 1).…”

Virgin coconut oil protected the diameter and the epithelium thickness of seminiferous tubules of mice (Mus musculus) from oral ethanol induction

“…Based on a few studies, the decrease of seminiferous tubules' diameter occured due to the destruction of germinal cells from germinative epithelium, which eventually decreased the epithelium thickness of the seminiferous tubules. The decrease in diameter and epithelium thickness might also occur due to the low number of cells that make up seminiferous tubules due to apoptosis (En et al, 2020).…”