The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa

Abstract

Objectives Staphylococcus aureus and Pseudomonas aeruginosa were the most common bacteria in nosocomial infections. Different bacteriocins are currently being studied as antibiotics or in conjunction with antibiotics as potential strategies to treat resistant infectious agents. The study aimed to determine nisin's effect on the biofilm production, antimicrobial susceptibility, and biofilm formation of S. aureus and P. aeruginosa. Materials and methods The experimental research tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The experimental study tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The MIC of bacteriocin nisin was determined using the micro broth dilution method, and crystal violet was used to assess the effect of bacteriocin on the biofilm. In addition, L929 cell culture was used to determine the effectiveness of bacteriocin on the isolate under similar cell conditions. Moreover, the MTT assay was used to and evaluate bacteriocin toxicity. In this study, the software Prism version 9 and Graph pad software were utilized. Results The results of this study reveal that the nisin has different activities at different doses and is considered dose-dependent. At various times and doses, nisin inhibits biofilm formation in S. aureus, and P. aeruginosa isolates. Nisin also showed a decreasing survival of the isolates. Antibiotic-resistant bacteria can be made more vulnerable by nisin. Furthermore, nisin treatment affected the production of virulence factors such as hemolysins in S. aureus and had little or a negative effect on P. aeruginosa virulence factors. This medication stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage. Conclusions Antibacterial properties of nicin against S. aureus and P. aeruginosa were successfully studied. This bacteriocin stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage or death. Damage to the membrane among the fundamental causes is reduced membrane potential and enzyme inactivation.

Evaluation of antibacterial activity of ozonated oil and ozonated water against Neisseria gonorrhoeae and Neisseria meningitidis by broth microdilution method

“…On the other hand, ozone gas had a more negligible effect, and therefore, it appears that it cannot be used extensively for disinfection. Prolonged exposure (40 min) failed to reduce resistant cell count [25][26][27][28]. Widespread access to oils such as sunflower, olive, and sesame, makes ozonated oil a competitive antimicrobial compound.…”

Interaction between plants and epiphytic lactic acid bacteria that affect plant silage fermentation

“…Bacteriocins can inhibit pathogens and toxins in plant. Such as, Nisin produced by LAB can inhibit the growth of Pseudomonas aeruginosa and cause cellular damage or death ( Mazzotta et al, 1997 ; Ghapanvari et al, 2022 ) while Lactocidin and nisin have inhibitory effects on Xanthomonas campestris , Erwinia carotovora , and Pseudomonas syringae ( Visser et al, 1986 ). When combined with EDTA, nisin shows enhanced antimicrobial activity against Gram-positive and some Gram-negative pathogens, such as X. campestris , E. carotovora ( Wells et al, 1998 ; Belfiore et al, 2007 ).…”

Nisin: Prerequisites and Prospects for Application in the Medical Field

“…However, it should be noted that there are conflicting data regarding certain bacteria. On the one hand, the antimicrobial efficacy of nisin against the entire genus Staphylococcus is reported, on the other hand, there is evidence of its selective activity against representatives of this genus (e.g., nisin exhibits bacteriostatic activity against MRSA and does not inhibit the growth of S. aureus ATCC 25937) [4,5,9,13,27,[32][33][34][35][36]. Gram-negative bacteria are usually much less sensitive or resistant to nisin.…”