The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots

Bensaddek, Lamine; Gillet, Françoise; Saucedo, J. E. Nava; Fliniaux, Marc‐André

doi:10.1016/s0168-1656(00)00372-2

Cited by 60 publications

(32 citation statements)

References 14 publications

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“…Our results are similar to that of cell suspension cultures of Catharanthus roseus to produce steroidal alkaloids (Panda et al 1992), of Taxus yunnanensis to produce taxol (Chen et al 2003) and that of hairy root cultures of Atropa belladonna to produce tropane alkaloids (Bensaddek et al 2001). Ammonium is very diffusive and easily accumulates into the tissues, becoming toxic if not immediately metabolized (Bensaddek et al 2001).…”

Section: Discussionsupporting

confidence: 81%

Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

Pan

Liu

et al. 2004

Biotechnology Letters

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Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1), respectively.

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Section: Discussionsupporting

confidence: 81%

Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

Pan

Liu

et al. 2004

Biotechnology Letters

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“…Most investigations in the literature have suggested that the concentration of nitrogen (39.5 mM NO 3 -and 20.5 mM NH 4 ? ), especially of ammonium, in MS medium is too high for the growth of root cultures (Bensaddek et al 2001;Wu et al 2006). Danesh et al (2006) have reported that high amount of ammonium in full-strength MS medium caused a rapid drop in the pH of the culture medium, thus reducing the growth of carrot roots.…”

Section: Resultsmentioning

confidence: 99%

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

et al. 2013

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Hairy roots of Dracocephalum moldavica L. were induced using Agrobacterium rhizogenes strain A4. Transformed roots were obtained from shoot explants with low transformation frequency of up to 3 %. The effects of different liquid media: Murashige and Skoog (MS), Gamborg et al. (B5) and Woody Plant (WP) with full-and half-strength ( MS, B5, WP), on biomass accumulation and rosmarinic acid (RA) content were investigated. The hairy roots were cultured in photoperiod (16 h light/8 h dark) and darkness. Biomass of D. moldavica hairy roots was the highest (7.23 g flask -1 of fresh weight and 0.89 g flask -1 of dry weight) in the cultures grown in WP medium under periodic light. Ultra performance liquid chromatography analysis revealed the highest RA content (78 mg g -1 dry wt) in roots cultured in B5 medium under photoperiod conditions. It was about tenfold higher compared to roots of field-grown mother plants. Antioxidant activities and total phenolic contents of methanolic extracts of D. moldavica hairy roots cultured in B5 and WP media under photoperiod and darkness and roots of field grown plants were compared. All extracts were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and phosphomolybdenum reduction assays. Total phenolic contents were estimated by the FolinCiocalteu method. The methanolic extract of D. moldavica hairy roots grown in B5 medium under photoperiod possessed the strongest effects on reducing Mo and DPPH radical scavenging. The activities were significantly higher (p B 0.05) than those of methanolic extract of roots of intact plants grown in the field. The most active methanolic extract of hairy roots was characterized by the highest level of rosmarinic acid and total content of phenolic compounds.

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“…However, with regard to in vitro conditions, the number of studies is more restricted. According to Bensaddek et al (2001), Atropa belladonna hairy roots growth, in terms of dry weight, was greater when the roots were maintained in medium with five times less ammonium than nitrate. Higher concentrations of ammonium lead to a 2.6-fold decrease in growth compared to the control.…”

Section: Resultsmentioning

confidence: 99%

Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

et al. 2008

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Six-year-old Levisticum officinale (lovage) hairy root cultures were used to study the effect of eight different NH(4) (+):NO(3) (-) ratios on their growth and volatile components. All cultures were kept at 24 degrees C on orbital shakers at 80 rpm, in darkness or in a 16 h light/8 h dark photoperiod. Growth was evaluated by dry and fresh weight determination. The volatiles were isolated by distillation-extraction and analysed by GC and GC-MS. Greater growth was attained in darkness with 10:90 (control, SH medium), 50:50 and 25:75 NH(4) (+):NO(3) (-) ratios, and also with SH control medium under the photoperiod condition, with a 10, 14, 12.5 and 12.5 fold increase of biomass in terms of dry weight, respectively, at the end of 42 days of growth. UPGMA cluster analysis of the mixtures of volatiles isolated from the hairy roots grown with different NH(4) (+):NO(3) (-) ratios confirmed their chemical variability. Although no particular grouping was detected in relation to the NH(4) (+):NO(3) (-) ratios or light conditions studied, most of the mixtures of volatiles isolated from the hairy roots were either dominated by n-octanal, (Z)-falcarinol or both components in about the same relative amounts.

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The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots

Cited by 60 publications

References 14 publications

Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

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