The Effect of Nitrocellulose Membrane Pore Size of Lateral Flow Immunoassay on Sensitivity for Detection of Shigella sp. in Milk Sample

Yahaya, Mohammad Lukman; Zakaria, Norhayati; Noordin, Rahmah; Razak, Khairunisak Abdul

doi:10.1016/j.matpr.2019.06.384

Cited by 13 publications

(12 citation statements)

References 10 publications

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“…High capillary flow times allow for a longer interaction time between the target molecule and the bioreceptor, thus increasing the sensitivity of the test. 157 At the same time, high capillary flow times also increase the chance for nonspecific binding to happen. Therefore, it is crucial to evaluate the membranes, with particular attention to the different capillary flow times, during the development of an LFA.…”

Section: Membranementioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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Lateral flow assays (LFA) are quick, simple and cheap assays to analyse a variety of samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample within a series of sequential pads with different functionalities aiming to generate a signal indicating the absence/presence (and, in some cases, the concentration) of the analyte of interest. In order to have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this Tutorial we provide the readers with: 1) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, 2) a roadmap for optimal LFA development independent of the specific application, 3) a step by step example protocol for the assembly and operation of an LF strip for the detection of Human Immunoglobulin G and 4) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs.

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Section: Membranementioning

confidence: 99%

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

et al. 2020

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“…The lowest detectable TL in HF90 has been barely detected by the naked eyes. The HF135 was the greatest selection since it gave high-intensity colors and line shape for the determination of S. flexneri to screen foodstuff contamination (M. Yahaya et al, 2019).…”

Section: Shigellamentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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“…from complex matrices like milk and formed the basis for detection of antigens from urine samples. 89 Using different materials like cellulose fiber for sample pad and coating polymers, like poly(vinyl alcohol), on the NC membranes have been reported to delay the flow of biomolecules (proteins) through the capillary channels, thus increasing sensitivity and providing a POC solution with shorter turnaround time. 90 Another easy and simple approach was adopted by integrating a sponge shunt into the LFA to decrease the fluid rate for increased interaction time between the analytes and reporter molecules.…”

Section: Development In Lfa Designmentioning

confidence: 99%

“…Effect of membrane porosity was evidently shown in detection of Shigella spp. from complex matrices like milk and formed the basis for detection of antigens from urine samples . Using different materials like cellulose fiber for sample pad and coating polymers, like poly(vinyl alcohol), on the NC membranes have been reported to delay the flow of biomolecules (proteins) through the capillary channels, thus increasing sensitivity and providing a POC solution with shorter turnaround time .…”

Section: Recent Advances In Lfas For Biomarker Detectionmentioning

confidence: 99%

Assessment of Urinary Biomarkers for Infectious Diseases Using Lateral Flow Assays: A Comprehensive Overview

et al. 2022

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Screening of biomarkers is a powerful approach for providing a holistic view of the disease spectrum and facilitating the diagnosis and prognosis of the state of infectious diseases. Unaffected by the homeostasis mechanism in the human body, urine accommodates systemic changes and reflects the pathophysiological condition of an individual. Easy availability in large volumes and non-invasive sample collection have rendered urine an ideal source of biomarkers for various diseases. Infectious diseases may be communicable, and therefore early diagnosis and treatment are of immense importance. Current diagnostic approaches preclude the timely identification of clinical conditions and also lack portability. Point-of-care (POC) testing solutions have gained attention as alternative diagnostic measures due to their ability to provide rapid and on-site results. Lateral flow assays (LFAs) are the mainstay in POC device development and have attracted interest owing to their potential to provide instantaneous results in resource-limited settings. The discovery and optimization of a definitive biomarker can render POC testing an excellent platform, thus impacting unwarranted antibiotic administration and preventing the spread of infectious diseases. This Review summarizes the importance of urine as an emerging biological fluid in infectious disease research and diagnosis in clinical settings. We review the academic research related to LFAs. Further, we also describe commercial POC devices based on the identification of urinary biomarkers as diagnostic targets for infectious diseases. We also discuss the future use of LFAs in developing more effective POC tests for urinary biomarkers of various infections.

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The Effect of Nitrocellulose Membrane Pore Size of Lateral Flow Immunoassay on Sensitivity for Detection of Shigella sp. in Milk Sample

Cited by 13 publications

References 10 publications

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Assessment of Urinary Biomarkers for Infectious Diseases Using Lateral Flow Assays: A Comprehensive Overview

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