The effect of nitrogen limitation on the physiology and metabolism of chlorella vulgaris var L3

Ikaran, Ziortza; Suárez-Álvarez, Sonia; Urreta, Iratxe; Castañón, Sonia

doi:10.1016/j.algal.2015.04.023

Cited by 100 publications

(43 citation statements)

References 59 publications

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“…Our findings showing this early increase in FA synthesis are in disagreement with the generally accepted hypothesis that the time lag between the onset of nitrogen stress and TG accumulation is related to the partitioning of carbon between carbohydrate and lipid synthesis (52,53). Approximately two-thirds of the lipidome-wide influx of newly synthesized FA moieties entered the membrane lipids in the first 2 days of nitrogen stress.…”

Section: Tg Is Produced Mainly By the Sequential Acylation Of Glycerocontrasting

confidence: 57%

Carbon and Acyl Chain Flux during Stress-induced Triglyceride Accumulation by Stable Isotopic Labeling of the Polar Microalga Coccomyxa subellipsoidea C169

Allen

DiRusso

Black

2017

Journal of Biological Chemistry

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Edited by George M. CarmanDeriving biofuels and other lipoid products from algae is a promising future technology directly addressing global issues of atmospheric CO 2 balance. To better understand the metabolism of triglyceride synthesis in algae, we examined their metabolic origins in the model species, Coccomyxa subellipsoidea C169, using stable isotopic labeling. Labeling patterns arising from [U- The incorporation rates of de novo synthesized FA into lipid classes were measured over a time course of nitrogen starvation. The synthesis of triglycerides, phospholipids, and galactolipids followed a two-stage pattern where nitrogen starvation resulted in a 2.5-fold increase followed by a gradual decline. Acyl chain flux into membrane lipids was dominant in the first stage followed by triglycerides. These data indicate that the level of metabolic control that determines acyl chain flux between membrane lipids and triglycerides during nitrogen stress relies primarily on the Kennedy pathway and de novo FA synthesis with limited, defined input from acyl editing reactions.

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Section: Tg Is Produced Mainly By the Sequential Acylation Of Glycerocontrasting

confidence: 57%

Carbon and Acyl Chain Flux during Stress-induced Triglyceride Accumulation by Stable Isotopic Labeling of the Polar Microalga Coccomyxa subellipsoidea C169

Allen

DiRusso

Black

2017

Journal of Biological Chemistry

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“…To evaluate the differences in storage product accumulation between oleaginous and non-oleaginous microalgae during N-depletion, we compared the published physiological and transcriptomic data of three widely studied oleaginous species: Nannochloropsis oceanica 740, Chlamydomonas reinhardtii 174142, and Chlorella vulgaris 43 (Figs 5 and 6). D. tertiolecta accumulates very low amounts of TAGs in N-replete and N-deplete conditions (0.2 and 1% DCW respectively), but stores a large amount of starch, thus making it a high starch and low TAG accumulator (Fig.…”

Section: Resultsmentioning

confidence: 99%

Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Tan

Lin

Shen

et al. 2016

Sci Rep

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Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of nitrogen, produced only 1% of dry cell weight (DCW) in neutral lipids, while starch was rapidly accumulated up to 46% DCW. The increased in starch content was accompanied by a coordinated overexpression of genes shunting carbon towards starch synthesis, a response not seen in the oleaginous microalgae Nannochloropsis oceanica, Chlamydomonas reinhardtii or Chlorella vulgaris. Genes in the central carbon metabolism pathways, particularly those of the tricarboxylic acid cycle, were also simultaneously upregulated, indicating a robust interchange of carbon skeletons for anabolic and catabolic processes. In contrast, fatty acid and triacylglycerol synthesis genes were downregulated or unchanged, suggesting that lipids are not a preferred form of storage in these cells. This study reveals the transcriptomic influence behind storage reserve allocation in D. tertiolecta and provides valuable insights into the possible manipulation of genes for engineering microorganisms to synthesize products of interest.

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“…Nitrogenous compounds are important for protein and Chl-a, Chl-b production [38,39]. When nitrogen is limited, significant decline in the cell division rate and low optical density value was found in the Fogg's Nitrogen free medium.…”

Section: Resultsmentioning

confidence: 99%

Growth Medium Screening for Chlorella vulgaris Growth and Lipid Production

Wong¹

2017

JAMB

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The effect of nitrogen limitation on the physiology and metabolism of chlorella vulgaris var L3

Cited by 100 publications

References 59 publications

Carbon and Acyl Chain Flux during Stress-induced Triglyceride Accumulation by Stable Isotopic Labeling of the Polar Microalga Coccomyxa subellipsoidea C169

Carbon and Acyl Chain Flux during Stress-induced Triglyceride Accumulation by Stable Isotopic Labeling of the Polar Microalga Coccomyxa subellipsoidea C169

Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Growth Medium Screening for Chlorella vulgaris Growth and Lipid Production

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