The Effect of Non Freeze-dried Hydrogel-CHA on Fibroblast Proliferation

Abstract

Bone damage can be caused by variety of surgical procedures. Bone reconstruction has been developed lately is tissue engineering techniques. One of materials that proved to be effective as a scaffold in tissue engineering is a hydrogel. The addition of carbonate apatite (CHA) will produce a hydrogel-CHA material which is believed to improve the mechanical properties and biological similarities with the original bone. Scaffold is considered an important aspect in the field of tissue engineering, because it's ability to mimic extracellular matrix of the damaged tissue. Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelial-mesenchymal interactions, secreting various growth factors and cytokines. On certain condition, Fibroblast will differentiate into bone-forming cells, osteoblasts. Objective: to determine the effect of non freezedried hydrogels -CHA on the number of fibroblasts. Methods: In the treatment groups (hydrogel and hydrogel-CHA group), the static seeding, where cells and scaffolds were simply brought into contact, was performed. The other group contained only cells and growth media. Cells were seeded at a density of 2x10 4 cells/ml in a 96-well plate. Number of fibroblasts cell in each group was observed by light microscopy and quantitified by MTT assay on days 1, 2 and 3 post-application. Results: Proliferation of fibroblasts increased significantly on day 3 rd after application of non freeze-dried hydrogel -CHA (p< 0.05). Conclusion: Application of non freeze-dried hydrogel -CHA may induce fibroblasts proliferation. ABSTRAKPengaruh aplikasi non freeze-dried hidrogel-CHA terhadap prolifrasi fibroblas. Kerusakan tulang dapat disebabkan oleh berbagai prosedur bedah. Rekonstruksi tulang yang banyak dikembangkan akhir-akhir ini adalah teknik rekayasa jaringan. Material yang terbukti efektif sebagai perancah dalam rekayasa jaringan adalah hidrogel. Penambahan Karbonat apatit (CHA) akan menghasilkan material hidrogel-CHA yang diyakini dapat meningkatkan sifat mekanis dan kemiripan biologis dengan tulang. Perancah merupakan aspek penting dalam bidang rekayasa jaringan, karena kemampuannya menyerupai matriks ekstraseluler pada jaringan yang rusak. Fibroblas merupakan sel mesenkim yang dengan mudah dibiakkan di laboratorium dan memiliki peran penting dalam interaksi epitel-mesenkimal, mensekresi berbagai faktor pertumbuhan dan sitokin. Pada kondisi tertentu fibroblas akan berdiferensiasi menjadi sel pembentuk tulang yaitu osteoblas. Tujuan: untuk mengetahui pengaruh non freeze-dried hidrogel-CHA terhadap jumlah sel fibroblas. Metode: Pada kelompok perlakuan (kelompok hidrogel dan hidrogel-CHA), penyemaian statis yaitu sel dan perancah dikontakkan. Pada kelompok lain hanya berisi sel dan media pertumbuhan. Sel disemai dengan kepadatan 2x10 4 sel/ml dalam 96 sumuran. Jumlah sel fibroblas dalam tiap kelompok diamati dengan menggunakan mikroskop dan dihitung dengan uji MTT pada hari 1, 2,dan 3 setelah aplikasi perlakuan. Hasil: Proliferasi...

The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

“…20 However, the results of this study are in line with research by Ardhiyanto and Siswomihardjo 23 that shows that after the implantation of hydroxyapatite on the 14th day, the amount of collagen continues to increase until the 28th day. This condition is caused by the CHA providing the right microenvironment to repair bone resorption of tissue 24 and to eliminate the inflammation. 25 Thus, collagen becomes more dense and alveolar bone regeneration occurs.…”

The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

“…The apatite carbonate gelatin material has been reported in several studies on its excellent biocompatibility, osteoconductivity, and increases the local concentration of calcium and phosphate ions needed for bone tissue regeneration. 8 The period of 30 days in this current study was selected because, in a study comparing the effectiveness of chlorhexidine varnish and chips in chronic periodontitis patients, significant differences were seen only in the 30 th day after treatment, while the results at three months and six months after treatment did not show any differences. 11 The apatite carbonate membrane gelatin can be well biodegraded and have excellent biocompatibility, resembling a bone connective tissue collagen matrix, which may initiate increased attachment and cell spread, and may also increase proliferation of fibroblasts.…”

“…The same material also plays an essential role in the regeneration of bone tissue. 8 The use of this membrane is expected to achieve better treatment efficacy than without additional therapy 9 , with the 5, or 15% CO 2 -containing N 2 atmosphere by a wet-chemical method with a strong ammonia alkali solution of calcium nitrate and diammonium hydrogen phosphate (5:3 in molar ratio). The purpose of this study was to investigate the effect of apatite carbonate membrane gelatin application on the levels of IL-1β in the gingival sulcus fluid of chronic periodontitis patients.…”