PurposeThis study aimed to compare the performance of sustainable shoes made with bacterial cellulosic composite and commercial leather shoes using an experimental research design. The two specific research objectives were: (1) to examine the basic material properties of multi-layered bacterial cellulosic materials (MBC), which include green tea-based cellulosic (GBC) mats, hemp fabrics, and denim fabrics, in comparison with those of two-layered leathers (MCP) consisting of calf-skin and pig-skin – commonly used in shoe manufacturing; and (2) to explore wearers’ performance in the two types of shoes by assessing quantitative kinematic and kinetic parameters of lower body movements.Design/methodology/approachThis study focused on assessing the basic materials testing and performance of sustainable shoes through a biomechanical approach, in contrast to commercially available leather shoes, through human wear trials. In this study, green tea-based cellulosic (GBC) mats were developed using the optimal combination of ingredients for cellulose growth. Subsequently, the GBC, denim fabric (100% cotton), and 100% hemp fabric were combined to create multi-layered bacterial cellulosic materials (MBC) as an alternative to leather. Additionally, calf-skin and pig-skin leathers were utilized to produce a commercially available two-layered leather (MCP), commonly employed in shoe manufacturing. 37 of the 42 human subjects who participated in wear testing were collected. A paired t-test was conducted to determine whether significant mean differences existed between the two shoe types, a paired t-test was conducted.FindingsTo develop a biodegradable and compostable material that could be used as a leather alternative for the footwear industry, we proposed MBC and examined its properties compared with those of MCP, a product often used when making shoes. These findings confirmed the similar properties of MBC and MCP from the material testing and the possibility of using a men’s sustainable shoe prototype as a leather alternative, in terms of kinematics and kinetics.Practical implicationsThe new multi-layered bacterial cellulosic materials (MBC) could be an alternative to commercial leathers such as innovative sustainable material construction, advanced design, and advanced techniques to optimize the overall performance of sustainable footwear.Originality/valueInvestigating the integration of smart textile technologies, ergonomic design principles, and personalized customization will contribute to developing MBC and making sustainable shoes using MBC compared with commercial leather shoes. This study provides valuable insights into further refinement and innovation in the sustainable footwear industry.