The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Abstract

Atmospheric pressure cold plasma (ACP) is introduced as a useful tool in a variety of biological applications. Proteins are the most abundant macromolecules in living systems with a central role in all biological processes. These organic molecules are modified by ACP exposure that is responsible for many of ACP’s biological effects. This study evaluated the effect of ACP on the production of recombinant phytase in yeast Pichia pastoris (P. pastoris) as well as the structure and function of the phytase enzyme. The results indicated that yeast cells treated with ACP, directly or indirectly, produced higher amounts of recombinant phytase, which was associated with the time of ACP treatment. The exposure of commercial phytase solution with ACP caused a significant increase in the enzyme activity (125%) after 4 hours. Evaluation of the phytase solution by far- and near-UV circular dichroism (CD) and fluorescence analysis indicated that this protein maintained its secondary structure when exposed to ACP while the tertiary structure was slightly unfolded. The effects of heat and H2O2 on the phytase structure and function were compared with the effect of ACP treatment. The modification of Cys, Tyr and Trp amino acids upon reactive oxygen/nitrogen spices was simulated using a molecular dynamics approach. RMSF and RMSD analysis suggested that this structural alteration occurs owing to changes made by reactive species in accessible amino acids.

The effect of cold atmospheric plasma on diabetes-induced enzyme glycation, oxidative stress, and inflammation; in vitro and in vivo

“…6, the argon plasma jet is containing both hydroxyl, and singlet oxygen radicals. RONS has two-edged sword nature in biology, as it could be either beneficial or harmful to biological systems depending on plasma properties (such as plasma source type, mode of energy dissipation into the system, feeding gas, plasma plum, and types and concentrations of reactive species), type of organism, and exposure time 3,6 . However, in the present study, the physiological characteristic of plasma is used as a positive induction to modify denatured protein structure and enzymatic activity.…”

“…In the non-thermal plasma, the species are in nonthermal equilibrium. Despite the existence of high-temperature electrons, the overall temperature of plasma, neutrals, ions, and radicals remain constant and close to room temperature; therefore, they are known as cold plasmas, with hindered macro heating of material that they contact with 3 . So, this feature makes the cold plasma suitable for many applications.…”

Plasma‐Driven in Situ Production of Hydrogen Peroxide for Biocatalysis

“…It is worth mentioning here that the extent of deleterious effects of plasmas are dependent on the enzyme. Some enzymes even exhibited increased activity after plasma treatment …”

Chemical mechanisms of non-thermal plasma action on cells

“…Экспериментально установлено, что ХП выступает в качестве генератора активных форм кислорода -ионов кислорода, свободных радикалов, а также перекиси водорода [3]. Основными стабильными продуктами (с временем полужизни более 1 с) плазмохимических реакций в барьерном разряде в атмосферном воздухе являются озон (О 3 ) и оксиды азота различной валентности (NО х ), которые формируются в соотношении 100:1 при малых энергиях и до 10 [4,6].…”