The effect of noradrenaline, injected into the hypothalamus, on thermoregulation in the cat.

Cooper, K. E.; Jones, Douglas L.; Pittman, QJ; Veale, W. L.

doi:10.1113/jphysiol.1976.sp011554

Cited by 26 publications

(5 citation statements)

References 19 publications

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“…Notably, after removal from the thermoneutral recording chamber, the body temperature dropped significantly. This drop is consistent with prior studies that show central NA outflow to the hypothalamus negatively regulates body temperature [ 82 , 83 ]. The magnitude of changes seen for both stimulation and inhibition of TgDBH_Cre defined NA neurons in various breathing parameters, particularly for hypercapnia, is in line with expectations of a highly redundant chemosensory system in the respiratory network along with the fact that the NA system is neuromodulatory in nature [ 37 , 52 , 84 – 87 ].…”

Section: Discussionsupporting

confidence: 93%

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

et al. 2022

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Background The functional understanding of genetic interaction networks and cellular mechanisms governing health and disease requires the dissection, and multifaceted study, of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgenic effector alleles represents a significant and powerful approach to delineate cell populations for functional, molecular, and anatomical studies. In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, its experimental implementation has been broadly restricted to manipulations of a limited set of common alleles that are often commercially produced at great expense, with costs and technical challenges associated with production of intersectional mouse lines hindering customized approaches to many researchers. Here, we present a simplified CRISPR toolkit for rapid, inexpensive, and facile intersectional allele production. Results Briefly, we produced 7 intersectional mouse lines using a dual recombinase system, one mouse line with a single recombinase system, and three embryonic stem (ES) cell lines that are designed to study the way functional, molecular, and anatomical features relate to each other in building circuits that underlie physiology and behavior. As a proof-of-principle, we applied three of these lines to different neuronal populations for anatomical mapping and functional in vivo investigation of respiratory control. We also generated a mouse line with a single recombinase-responsive allele that controls the expression of the calcium sensor Twitch-2B. This mouse line was applied globally to study the effects of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) on calcium release in the ovarian follicle. Conclusions The lines presented here are representative examples of outcomes possible with the successful application of our genetic toolkit for the facile development of diverse, modifiable animal models. This toolkit will allow labs to create single or dual recombinase effector lines easily for any cell population or subpopulation of interest when paired with the appropriate Cre and FLP recombinase mouse lines or viral vectors. We have made our tools and derivative intersectional mouse and ES cell lines openly available for non-commercial use through publicly curated repositories for plasmid DNA, ES cells, and transgenic mouse lines.

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Section: Discussionsupporting

confidence: 93%

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

et al. 2022

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“…Notably, after removal from the thermo-neutral recording chamber, the body temperature dropped significantly. This drop is consistent with prior studies that show central NA outflow to the hypothalamus negatively regulates body temperature (83,84). The magnitude of changes seen for both stimulation and inhibition of TgDBH_Cre defined NA neurons in various breathing parameters, particularly for hypercapnia, is in line with expectations of a highly redundant chemosensory system in the respiratory network along with the fact that the NA system is neuromodulatory in nature (37,52,(85)(86)(87)(88).…”

Section: Select Mouse Line Characterization: Intersectional Expression Of Fluorescent Proteins (Fps) Hassupporting

confidence: 91%

A CRISPR toolbox for generating intersectional genetic mice for functional, molecular, and anatomical circuit mapping

Lusk

McKinney

et al. 2021

Preprint

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Background A full understanding of circuits and cellular mechanisms governing health and disease requires the dissection and multi-faceted study of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgenic response alleles represents a significant and powerful approach to delineate cell populations for functional, molecular, and anatomical study. In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, the expense and difficulty associated with production of customized intersectional mouse lines have limited widespread application to more common allele manipulations that are often commercially produced at great expense. Results We present a simplified CRISPR toolkit for rapid, inexpensive, and facile intersectional allele production. Briefly, we produced 7 intersectional mouse lines using a dual recombinase system, one mouse line with a single recombinase system, and three embryonic stem (ES) cell lines that are designed to study how functional, molecular, and anatomical features relate to each other in building circuits that underlie physiology and behavior. As a proof-of-principle, we applied three of these lines to different neuronal populations for anatomical mapping and functional in vivo investigation of respiratory control. We also generated a mouse line with a single recombinase responsive allele that controls the expression of the calcium sensor Twitch-2B. This mouse line was applied globally to study the effects of follicle stimulating hormone (FSH) and luteinizing hormone (LH) on calcium release in the ovarian follicle. Conclusions Lines presented here are representative examples of outcomes possible with the successful application of our genetic toolkit for the facile development of diverse, modifiable animal models. This toolkit will allow labs to create single or dual recombinase effector lines easily for any cell population or subpopulation of interest when paired with the appropriate Cre and FLP recombinase mouse lines or viral vectors. We have made our tools and derivative intersectional mouse and ES cell lines openly available for non-commercial use through publicly curated repositories for plasmid DNA, ES cells, and transgenic mouse lines.

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“…Both NA and 5-HT are neurotransmitters associated with temperature regulation ( Feldberg and Myers, 1963 ; Clark and Lipton, 1986 ; Cooper, 2002 ). For example, NA and 5-HT injection into the hypothalamus decreases temperature and heat production ( Cooper et al, 1976 ; Cox et al, 1980 ; Lin et al, 1983 ). These experiments could, in part, explain the increase in hypothalamic NA and 5-HT levels ( Kandel et al, 2013 ) in our study.…”

Section: Discussionmentioning

confidence: 99%

Traumatic brain injury extending to the striatum alters autonomic thermoregulation and hypothalamic monoamines in recovering rats

Verduzco-Mendoza,

Mota-Rojas,

Olmos Hernández

et al. 2023

Front. Neurosci.

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The brain cortex is the structure that is typically injured in traumatic brain injury (TBI) and is anatomically connected with other brain regions, including the striatum and hypothalamus, which are associated in part with motor function and the regulation of body temperature, respectively. We investigated whether a TBI extending to the striatum could affect peripheral and core temperatures as an indicator of autonomic thermoregulatory function. Moreover, it is unknown whether thermal modulation is accompanied by hypothalamic and cortical monoamine changes in rats with motor function recovery. The animals were allocated into three groups: the sham group (sham), a TBI group with a cortical contusion alone (TBI alone), and a TBI group with an injury extending to the dorsal striatum (TBI + striatal injury). Body temperature and motor deficits were evaluated for 20 days post-injury. On the 3rd and 20th days, rats were euthanized to measure the serotonin (5-HT), noradrenaline (NA), and dopamine (DA) levels using high-performance liquid chromatography (HPLC). We observed that TBI with an injury extending to the dorsal striatum increased core and peripheral temperatures. These changes were accompanied by a sustained motor deficit lasting for 14 days. Furthermore, there were notable increases in NA and 5-HT levels in the brain cortex and hypothalamus both 3 and 20 days after injury. In contrast, rats with TBI alone showed no changes in peripheral temperatures and achieved motor function recovery by the 7th day post-injury. In conclusion, our results suggest that TBI with an injury extending to the dorsal striatum elevates both core and peripheral temperatures, causing a delay in functional recovery and increasing hypothalamic monoamine levels. The aftereffects can be attributed to the injury site and changes to the autonomic thermoregulatory functions.

show abstract

The effect of noradrenaline, injected into the hypothalamus, on thermoregulation in the cat.

Cited by 26 publications

References 19 publications

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

A CRISPR toolbox for generating intersectional genetic mice for functional, molecular, and anatomical circuit mapping

Traumatic brain injury extending to the striatum alters autonomic thermoregulation and hypothalamic monoamines in recovering rats

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