The effect of normal, metaplastic, and neoplastic esophageal extracellular matrix upon macrophage activation

Saldin, Lindsey T.; Klimak, Molly; Hill, Ryan C.; Cramer, Madeline C.; Huleihel, Luai; Xue, Li; Quidgley-Martin, Maria; Cardenas, David; Keane, Timothy J.; Londoño, Ricardo; Hussey, George S.; Kelly, Lori A.; Kosovec, Juliann E.; Lloyd, Emily J.; Omstead, Ashten N.; Zhang, Li; Nieponice, Alejandro; Jobe, Blair A.; Hansen, Kirk C.; Zaidi, Ali H.; Badylak, Stephen F.

doi:10.1016/j.regen.2020.100037

Cited by 10 publications

(13 citation statements)

References 62 publications

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“… 62 The option of inducing a tumour is also possible. For example, Saldin and colleagues 63 induced oesophageal adenocarcinoma in Sprague-Dawley rats by subjecting them to the Levrat surgical procedure. In this procedure, the jejunum is anastomosed to the distal oesophagus, thereby creating constant acid reflux and chronic inflammation in the distal oesophagus.…”

Section: Decellularization Of Tumoursmentioning

confidence: 99%

“…The advantage of this procedure is that the authors could harvest the tumour tissue at different stages of the disease. 63 Some of the disadvantages of using tumour tissue isolated from human patients, for example, patient-specificity and tissue availability, are solved by using animal derived tumour tissue as a source. Nevertheless, ethical constraints are still a disadvantage of this tumour tissue source.…”

Section: Decellularization Of Tumoursmentioning

confidence: 99%

“…Therefore, researchers tend to focus on one or two cell types to slowly unravel their behaviour in the TME. 59 , 63 , 84 Technically, as with other decellularized materials, recellularization of dT-ECM can be accomplished by simply seeding the cell suspension onto the dT-ECM piece. 91 , 92 Culture of the seeded dT-ECM can be done statically or dynamically 90 , 91 and special attention should be paid to seeding efficiency, that is, percentage of seeded cells that actually attach to the dT-ECM scaffold, that may be optimized as done in other tissue engineering applications.…”

Section: Decellularization Of Tumoursmentioning

confidence: 99%

See 2 more Smart Citations

Decellularization of tumours: A new frontier in tissue engineering

2022

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Cancer is one of the leading causes of death worldwide. The tumour extracellular matrix (ECM) has unique features in terms of composition and mechanical properties, resulting in a structurally and chemically different ECM to that of native, healthy tissues. This paper reviews to date the efforts into decellularization of tumours, which in the authors’ view represents a new frontier in the ever evolving field of tumour tissue engineering. An overview of the ECM and its importance in cancer is given, ending with examples of research using decellularized tumours, which has already indicated potential therapeutic targets, unravelled malignancy mechanisms or response to chemotherapy agents. The review highlights that more research is needed in this area, which can answer important questions related to tumour formation and progression to ultimately identify new and effective therapeutic targets. Within the near-future of personalized medicine, this research can create patient-specific tumour models and therapeutic regimes.

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Section: Decellularization Of Tumoursmentioning

confidence: 99%

Section: Decellularization Of Tumoursmentioning

confidence: 99%

Section: Decellularization Of Tumoursmentioning

confidence: 99%

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Decellularization of tumours: A new frontier in tissue engineering

2022

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“…Cells of the immune system, such as tissue-resident macrophages, circulating monocytes, and lymphocytes are no exception to the influence of ECM. ECM derived from healthy tissues and configured into surgical mesh materials, topical powders, or hydrogels, has been shown to promote an anti-inflammatory, regulatory macrophage phenotype 4 – 15 .…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory matrix-bound nanovesicles mitigate acute and chronic pristane-induced rheumatoid arthritis

et al. 2022

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Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and destruction of synovial joints affecting ~7.5 million people worldwide. Disease pathology is driven by an imbalance in the ratio of pro-inflammatory vs. anti-inflammatory immune cells, especially macrophages. Modulation of macrophage phenotype, specifically an M1 to M2, pro- to anti-inflammatory transition, can be induced by biologic scaffold materials composed of extracellular matrix (ECM). The ECM-based immunomodulatory effect is thought to be mediated in part through recently identified matrix-bound nanovesicles (MBV) embedded within ECM. Isolated MBV was delivered via intravenous (i.v.) or peri-articular (p.a.) injection to rats with pristane-induced arthritis (PIA). The results of MBV administration were compared to intraperitoneal (i.p.) administration of methotrexate (MTX), the clinical standard of care. Relative to the diseased animals, i.p. MTX, i.v. MBV, and p.a. MBV reduced arthritis scores in both acute and chronic pristane-induced arthritis, decreased synovial inflammation, decreased adverse joint remodeling, and reduced the ratio of synovial and splenic M1 to M2 macrophages (p < 0.05). Both p.a. and i.v. MBV reduced the serum concentration of RA and PIA biomarkers CXCL10 and MCP-3 in the acute and chronic phases of disease (p < 0.05). Flow-cytometry revealed the presence of a systemic CD43hi/His48lo/CD206+, immunoregulatory monocyte population unique to p.a. and i.v. MBV treatment associated with disease resolution. The results show that the therapeutic efficacy of MBV is equal to that of MTX for the management of acute and chronic pristane-induced arthritis and, further, this effect is associated with modulation of local synovial macrophages and systemic myeloid populations.

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“…Several studies have documented the effects of mammalian non-neoplastic ECM upon neoplastic cells in vitro and in vivo. These studies have included several types of cancer including breast, urinary bladder, prostate, colon, skin, and esophageal, among others [8][9][10][11][12][13][14][15]. A recent study showed the beneficial therapeutic effects of an ECM hydrogel derived from normal esophageal tissues upon dysplastic esophageal mucosa (i.e.…”

Section: Introductionmentioning

confidence: 99%

A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo

et al. 2022

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Suppressive effects of extracellular matrix (ECM) upon various cancers have been reported. Glioblastoma multiforme has poor prognosis and new therapies are desired. This work investigated the effects of a saline-soluble fraction of urinary bladder ECM (ECM-SF) upon glioma cells. Viability at 24 hours in 1, 5, or 10 mg/mL ECM-SF-spiked media was evaluated in primary glioma cells (0319, 1015, 1119), glioma cell lines (A172, T98G, U87MG, C6), and brain cell lines (HCN-2, HMC3). Viability universally decreased at 5 and 10 mg/mL with U87MG, HCN-2, and HCM3 being least sensitive. Apoptosis in 0319 and 1119 cells was confirmed via NucView 488. Bi-weekly intravenous injection of ECM-SF (120 mg/kg) for 10 weeks in Sprague-Dawley rats did not affect weight, temperature, complete blood count, or multi-organ histology ( N = 5). Intratumoral injection of ECM-SF (10 uL of 30 mg/mL) at weeks 2–4 post C6 inoculation in Wistar rats increased median survival from 24.5 to 51 days (hazard ratio for death 0.22) and decreased average tumor volume at time of death from 349 mm 3 to 90 mm 3 over 10 weeks ( N = 6). Mass spectrometry identified 2,562 protein species in ECM-SF, parent ECM, and originating tissue. These results demonstrate the suppressive effects of ECM on glioma and warrant further study.

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The effect of normal, metaplastic, and neoplastic esophageal extracellular matrix upon macrophage activation

Cited by 10 publications

References 62 publications

Decellularization of tumours: A new frontier in tissue engineering

Decellularization of tumours: A new frontier in tissue engineering

Immunomodulatory matrix-bound nanovesicles mitigate acute and chronic pristane-induced rheumatoid arthritis

A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo

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