The effect of numbers of frozen-thawed boar sperm and addition of prostaglandin F2α at insemination on fertility in pigs

Knox, Robert V.; Yantis, Brandon M.

doi:10.1016/j.anireprosci.2014.10.018

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…Similarly, not all sperm additives, such as caffeine (Yamaguchi et al. ) or prostaglandin (Knox and Yantis ) have worked well to improve fertility when used with AI. Collectively, these data suggest that the conditions for improving the yield and fertility of frozen–thawed boar sperm is possible with the use of specific additives under defined conditions.…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

“…However, not all antioxidants have worked as well and might be attributed to the concentration and the freezing conditions (Buranaamnuay et al 2011a), or perhaps while improv-ing in vitro sperm motility and viability and IVF (Luño et al 2014) have only minimal effects on in vivo fertility (Luño et al 2015). Similarly, not all sperm additives, such as caffeine (Yamaguchi et al 2009) or prostaglandin (Knox and Yantis 2014) have worked well to improve fertility when used with AI. Collectively, these data suggest that the conditions for improving the yield and fertility of frozen-thawed boar sperm is possible with the use of specific additives under defined conditions.…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

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The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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Contents One of the limits to practical use of frozen boar sperm involves the lowered fertility when used for artificial insemination. Years of studies have shown that 5–6 billion sperm (approximately 3 billion viable) used in single or multiple inseminations results in pregnancy rates most often between 60 and 70% and with litter sizes between nine and 10 pigs. Yet today, it is not uncommon for studies to report pregnancy rates from 70 to 85% and litter sizes with 11–12 pigs. While global statements about the incidence and reasons for higher fertility are not conclusive, incremental fertility improvements appear independently associated with use of a minimum number of viable sperm (1–2 billion), insemination timing that increases the probability that sperm will be present close to ovulation for groups of females, selection for boar sperm survival following cryopreservation, and modification of the freeze and thaw conditions using additives to protect sperm from oxidative damage. Studies show that techniques such as intrauterine and deep uterine insemination can provide an opportunity to reduce sperm numbers and that control of time of ovulation in groups of females can reduce the need for multiple inseminations and improve the chance for AI close to ovulation. However, optimal and consistent fertility with cryopreserved boar sperm may require a multifaceted approach that includes boar selection and screening, strategic use of additives during the freezing and thawing process, post‐thaw evaluation of sperm and adjustments in sperm numbers for AI, assessment of female fertility and ovulation induction for single insemination. These sequenced procedures should be developed and incorporated into a quality control system for improved fertility when using minimal numbers of cryopreserved boar sperm.

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Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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“…The differences in motility of semen may be explained by the fact that the variation in the age of bulls, genetics, temperature and season of semen collection, feeding and semen collecting frequency between studies (Ismaya, 2014). Moreover, the variation in the quality of preserved frozen semen may be due to man-made manipulation during processing after its collection resulting in changes in sperm cell structures followed by poor sperm survival rate and fertilization capacity (Knox and Yantis, 2014). The proportion of spermatozoa with normal acrosome, midpiece and tail and with normal head morphology recorded in this study was 88.23±2.1 and 95.1±1.6%, respectively.…”

Section: Discussionmentioning

confidence: 67%

Quality of frozen semen of Brahman bulls used for routine artificial insemination in Bangladesh

Bhuiyan

Islam

Maoya

et al. 2019

BJVM

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Background: Semen must be of good quality to achieve satisfactory conception rate in any artificial insemination (AI) programme. The objectives of the present study were to evaluate the quality of frozen semen of Brahman bulls and compare the same semen derived from different sources. Methods: Fifteen frozen semen straws of Brahman bulls derived from 3 different sources (5 straws from each source) were evaluated with respect to volume, motility, concentration of spermatozoa and morphology of spermatozoa. Volume of semen was determined by micropipette, concentration was evaluated by haemocytometer technique, motility and progressive motility were evaluated by computer assisted sperm analyser (CASA), spermatozoa with normal acrosome, midpiece and tail were evaluated in formol-saline fixed semen and spermatozoa with normal head morphology were evaluated by Farley staining technique. Moreover, presence or absence of bacteria in semen was evaluated by Gram’s staining technique. Results: The overall semen volume, concentration, motile percentage, progressive motile percentage, percentage of spermatozoa with normal acrosome, midpiece and tail, and percentage of normal head morphology were 0.19±0.01 ml, 30.87±3.4x106/ml, 56.47±24.2 (%), 16.23±9.8 (%), 88.23±2.1 (%) and 95.1±1.6 (%), respectively. When compared, similar volume of semen (0.19 ml) was found in all frozen semen derived from different sources. The difference in percentage of motile spermatozoa between source B and C was significant (P<0.05). The difference in spermatozoa with normal acrosome, midpiece and tail in semen between source A and B was significant (P<0.05). The difference in the percentage of progressive motile spermatozoa, the concentration of spermatozoa and normal head morphology in semen among different sources of semen was not significant (P>0.05). The difference in percentage of motile spermatozoa, the percentage of progressive motile spermatozoa, the concentration of spermatozoa, normal acrosome, midpiece and tail, normal head morphology in semen between locally produced and foreign semen was not significant (P>0.05). All examined 15 semen samples were negative for presence of bacteria. Conclusions: Although the quality of frozen semen derived from Brahman bulls seems to be within normal limit, the post-AI conception rate should be determined to evaluate the fertilizing capacity of the used semen.

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“…It is highly sensitive to the effects of external factors . Ejaculate processing after its collection can cause changes in sperm cell structures, thus affecting sperm survival rate and fertilisation capacity ( Knox and Yantis, 2014 ). It is not a common insemination practice to analyse the changes that may occur in semen following its collection and dilution.…”

Section: Introductionmentioning

confidence: 99%

The Usefulness of Selected Physicochemical Indices, Cell Membrane Integrity and Sperm Chromatin Structure in Assessments of Boar Semen Sensitivity

Wysokińska¹,

Kondracki²,

Iwanina³

2015

Asian Australas. J. Anim. Sci

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The present work describes experiments undertaken to evaluate the usefulness of selected physicochemical indices of semen, cell membrane integrity and sperm chromatin structure for the assessment of boar semen sensitivity to processes connected with pre-insemination procedures. The experiments were carried out on 30 boars: including 15 regarded as providers of sensitive semen and 15 regarded as providers of semen that is little sensitive to laboratory processing. The selection of boars for both groups was based on sperm morphology analyses, assuming secondary morphological change incidence in spermatozoa as the criterion. Two ejaculates were manually collected from each boar at an interval of 3 to 4 months. The following analyses were carried out for each ejaculate: sperm motility assessment, sperm pH measurement, sperm morphology assessment, sperm chromatin structure evaluation and cell membrane integrity assessment. The analyses were performed three times. Semen storage did not cause an increase in the incidence of secondary morphological changes in the group of boars considered to provide sperm of low sensitivity. On the other hand, with continued storage there was a marked increase in the incidence of spermatozoa with secondary morphological changes in the group of boars regarded as producing more sensitive semen. Ejaculates of group I boars evaluated directly after collection had an approximately 6% smaller share of spermatozoa with undamaged cell membranes than the ejaculates of boars in group II (p≤0.05). In the process of time the percentage of spermatozoa with undamaged cell membranes decreased. The sperm of group I boars was characterised with a lower sperm motility than the semen of group II boars. After 1 hour of storing diluted semen, the sperm motility of boars producing highly sensitive semen was already 4% lower (p≤0.05), and after 24 hours of storage it was 6.33% lower than that of the boars that produced semen with a low sensitivity. Factors that confirm the accuracy of insemination male selection can include a low rate of sperm motility decrease during the storage of diluted semen, low and contained incidence of secondary morphological changes in spermatozoa during semen storage and a high frequency of spermatozoa with undamaged cell membranes.

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The effect of numbers of frozen-thawed boar sperm and addition of prostaglandin F2α at insemination on fertility in pigs

Cited by 8 publications

References 46 publications

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Quality of frozen semen of Brahman bulls used for routine artificial insemination in Bangladesh

The Usefulness of Selected Physicochemical Indices, Cell Membrane Integrity and Sperm Chromatin Structure in Assessments of Boar Semen Sensitivity

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