The effect of osmotic stress on the metaphase II spindle of human oocytes, and the relevance to cryopreservation.

Mullen, Steven F.; Ağca, Yüksel; Broermann, D.C.; Jenkins, C.L.; Johnson, Chad A.; Critser, John K.

doi:10.1093/humrep/deh201

Cited by 111 publications

(73 citation statements)

References 63 publications

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“…This has not been the case with developmental competence of cat oocytes matured in vitro that was severely impacted by exposure to 0.5 M sucrose (~800 mOsm) for 5 min only (Murakami et al, 2004). We suspect that metaphase plate and spindle of cat oocytes likely are highly sensitive to anisosmotic conditions as it has clearly been shown in mature human oocytes (Mullen et al, 2004). Interestingly, exposure of immature oocytes to dehydrating sucrose solutions for 15 min, wherein oocytes did not regain their initial volume unless returned to isotonic conditions, appeared to be more detrimental than exposures to permeating cryoprotectant solutions of similar osmolarity (e.g.…”

Section: Discussionmentioning

confidence: 85%

“…Another important prerequisite to developing a practical freezing protocol is understanding oocyte tolerance to radical changes in osmolarity that occur during adding and removing cryoprotectant and exposing the gamete to low temperatures (Critser et al, 1997). The resulting changes in cell volume have been shown to have significant effects on oocyte developmental competence in the mouse (Litkouhi et al, 1997), human (Newton et al, 1999;Mullen et al, 2004), cow (Agca et al, 2000), and rhesus monkey (Songsasen et al, 2002). However, no information is available on the impact of osmotic stress on the immature cat oocyte.…”

Section: Introductionmentioning

confidence: 99%

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Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Comizzoli

Wildt

Pukazhenthi

2007

Molecular Reproduction Devel

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During cryopreservation, the immature oocyte is subjected to anisosmotic conditions potentially impairing subsequent nuclear and cytoplasmic maturation in vitro. In preparation for cryopreservation protocols and to characterize osmotic tolerance, cat cumulus-oocyte complexes (COC) at the germinal vesicle (GV) stage were exposed for 15 min to sucrose solutions ranging from 100 to 2,000 mOsm and then examined for structural integrity and developmental competence in vitro. Osmolarities ≥200 and ≤750 mOsm had no effect on incidence of oocyte nuclear maturation, fertilization success, and blastocyst formation compared to control COC (exposed to 290 mOsm). This relatively high osmotic tolerance of the immature cat oocyte appeared to arise from a remarkable stability of the GV chromatin structure as well as plasticity in mitochondrial distribution, membrane integrity, and ability to maintain cumulus-oocyte communications. Osmolarities < 200 mOsm only damaged cumulus cell membrane integrity, which contributed to poor nuclear maturation but ultimately had no adverse effect on blastocyst formation in vitro. Osmolarities >750 mOsm compromised nuclear maturation and blastocyst formation in vitro via disruption of cumulus-oocyte communications, an effect that could be mitigated through 1,500 mOsm by adding cytochalasin B to the hyperosmotic solutions. These results (1) demonstrate, for the first time, the expansive osmotic tolerance of the immature cat oocyte, (2) characterize the fundamental role of cumulus-oocyte communications when tolerance limits are exceeded, and (3) reveal an interesting hyperosmotic tolerance of the immature oocyte that can be increased two-fold by supplementation with cytochalasin B.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Comizzoli

Wildt

Pukazhenthi

2007

Molecular Reproduction Devel

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“…Using the experimental data from the present study and osmotic tolerance data from our previous study (65), we have investigated methods to vitrify human oocytes using computer modeling. Our goal in the present work was to develop a method which should ensure ice-free cryopreservation (i.e.…”

Section: Experiments 3: Computer Modeling Toward An Optimal Vitrificatmentioning

confidence: 99%

“…Rhesus macaque oocytes suffered membrane damage after exposure to concentrations of EG at 3 mol/L or higher using a single-step addition and removal (50), although it was not determined if this damage occurred specifically as a result of the volume excursions, or from a chemical effect of the EG, or an interaction between these 2 factors. While it has been shown that human oocytes can tolerate exposure to fairly high concentrations of mono-and disaccharides (up to 1.5 mol/L; equivalent to a reduction in volume to ~35 % of isotonic (57)) as measured by immediate viability using membrane integrity and enzyme activity (83), a reduced tolerance was reported as measured by MII spindle morphology (65). To our knowledge, viability after osmotic stress, as measured by developmental potential, has not been reported for in vivo matured MII human oocytes.…”

Section: Ethylene Glycol Permeability and Its Use For Vitrificationmentioning

confidence: 99%

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Mullen

Mei

et al. 2008

Fertility and Sterility

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Objectives-To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.Design-An incomplete randomized block design was used for this study.Setting-A University-affiliated assisted reproductive center.Patients-Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong University.Interventions-Oocytes were exposed to 1.0 molar ethylene glycol in a single step, and photographed during subsequent volume excursions.Main outcome measures-A 2-parameter model was employed to estimate the permeability to water and EG.Results-Water permeability ranged from 0.15 to 1.17 µm/(min·atm), and ethylene glycol permeability ranged from 1.5 to 30 µm/min between 7 °C at 36 °C. The activation energies for water and ethylene glycol permeability were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Some of the results from this study were presented at the 62 nd annual meeting of the American Society for Reproductive Medicine, 21-25 October 2006, in New Orleans, LA USA. Conflict of Interest: None. CapsuleHuman metaphase II oocyte permeability to ethylene glycol was determined. A method for vitrification, based upon fundamental principles, was developed from these data. Conclusions-Despite the lower permeability of human MII oocytes to ethylene glycol compared to previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes with a vitrifiable concentration of ethylene glycol can be designed which prevent excessive osmotic stress and minimize exposure to high concentrations of this compound. NIH Public Access

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“…Indeed, cooling oocytes to room temperature for even as little as 10 minutes can cause irreversible damage to the meiotic spindle. Furthermore, chilling oocytes to 0°C in the presence of a CPA appears to exacerbate these abnormalities (Mullen et al, 2004).…”

Section: Cryobiology and Fertility Preservationmentioning

confidence: 99%

Preservation of female fertility in humans and animal species

Picton¹

2018

Anim Reprod

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A detailed understanding of the cryobiology of gametes and complex tissues has led to the development of methods that facilitate the successful low temperature banking of isolated mature human oocytes, or immature oocytes in situ within fragments of human ovarian cortex. Although many outstanding research challenges remain to be addressed, the successful development of new treatments to preserve female fertility for a range of clinical indications has largely been underpinned by the conduct of extensive, fundamental research on oocytes and ovarian tissues from a number of laboratory and commercially important farm species. Indeed, the most recent evidence from large animals suggests that it is also possible to cryopreserve intact whole ovaries along with their supporting vasculature for later autotransplantation and restoration of natural fertility. This review will explore how the methods developed to preserve human oocytes and ovarian tissues can now be used strategically to support the development of conservation strategies aimed at safeguarding the genetic diversity of commercially important domestic animals and also of preserving the female germplasm for wild animals and endangered species.

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The effect of osmotic stress on the metaphase II spindle of human oocytes, and the relevance to cryopreservation.

Abstract: Anisosmotic conditions lead to disruption of the MII spindle in human oocytes. Applying this fundamental knowledge to human oocyte cryopreservation should result in increased numbers of cells maintaining viability.

Cited by 111 publications

References 63 publications

Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Impact of anisosmotic conditions on structural and functional integrity of cumulus–oocyte complexes at the germinal vesicle stage in the domestic cat

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Preservation of female fertility in humans and animal species

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