Initiation of immune responses to helminth infection require interleukin (IL)-4 receptor signaling, activating signal transducer and activation of transcription 6 (STAT6) and resulting in elevated IL-4 and-13 production. This phenomenon occurs during early Haemonchus contortus (H. contortus) infection in parasite-resistant St. Croix (STC) sheep, yet the cell responsible for early cytokine production is unknown. Studies have reported greater infiltration of neutrophils to the abomasum of STC by 3 days after H. contortus infection as compared to parasite-susceptible Suffolk sheep (SUF). Neutrophils have been noted to play a role in immune responses to murine helminth infections through production of IL-13, which promotes alternative macrophage polarization. Thus, it is reasonable to hypothesize that neutrophils are an early source of IL-4/IL-13 that promote resistance to H. contortus infection. This study aimed to determine differences in ovine neutrophil responses when stimulated with antigen (Ag) from larval and adult stages of H. contortus. Neutrophils from STC and SUF sheep were cultured with either larval (HcLA) or adult (HcWA) antigen. In vitro production of (IL-4/IL-13) cytokine were measured using an ovine-specific enzyme-linked immunosorbent assay (ELISA). Neutrophils from either breed exposed to HcLA produced significantly higher levels of IL-4 by 30 minutes (STC, 3153.65 pg/ml and SUF, 4665.22 pg/ml) and IL-13 by 6 hours (STC, 391.02 pg/ml and SUF, 419.6 pg/ml) when compared to neutrophils cultured with HcWA (STC IL-4, 6.04 pg/ml and SUF, 8.05 pg/ml, respectively) (STC IL-13, 10 pg/ml and 12.5 pg/ml) (P < 0.001). While no breed differences were observed, these data indicate that neutrophils preferentially respond to HcLA compared to HcWA. Taken together these data indicate that neutrophils are a potential effector cell responsible for initiating early Th2 responses during H. contortus infection in sheep.