The effect of oxalic and itaconic acids on threo-Ds-isocitric acid production from rapeseed oil by Yarrowia lipolytica

Kamzolova, Svetlana V.; Allayarov, Ramil K.; Lunina, Julia N.; Morgunov, Igor G.

doi:10.1016/j.biortech.2016.01.092

Cited by 26 publications

(25 citation statements)

References 30 publications

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“…In further studies, the pH of the culture medium was maintained at a level of 6.0. Information on the pathway of ICA formation by wild and genetically modified microbial strains cultivated on n-alkanes, ethanol, or vegetable oils can be found in the literature [9,11,[13][14][15]46]. The authors of these papers emphasize the key role of isocitrate lyase, an enzyme of the glyoxylate cycle involved in the metabolism of ICA.…”

Section: Production Of Icamentioning

confidence: 99%

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Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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Within this work, the microbial synthesis of (2R,3S)-isocitric acid (ICA), a metabolite of the nonconventional yeast Yarrowia lipolytica, from biodiesel waste, has been studied. The selected strain Y. lipolytica VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions. The maximal production of ICA with the minimal formation of the byproduct was provided by the use of a concentration of (NH4)2SO4 (6 g/L); the addition of biodiesel waste to cultivation medium in 20–60 g/L portions; maintaining the pH of the cultivation medium at 6, and degree of aeration between 25% and 60% of saturation. Itaconic acid at a concentration of 15 mM favorably influenced the production of ICA by the selected strain. The optimization of cultivation conditions allowed us to increase the concentration of ICA in the culture liquid from 58.32 to 90.2 g/L, the product yield (Y) by 40%, and the ICA/CA ratio from 1.1:1 to 3:1. Research on laboratory animals indicated that ICA counteracted the negative effect of ammonium molybdate (10−5 М) and lead diacetate (10−7 М) on the learning and spatial memory of rats, including those exposed to emotional stress.

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Section: Production Of Icamentioning

confidence: 99%

“…The inhibition of isocitrate lyase with itaconic or oxalic acids, structural analogues of succinate and glyoxylate, shifted the process of acid formation towards the preferential formation of ICA. The best results were observed with itaconic acid added to the medium at a concentration of 30-40 mM [46].…”

Section: Production Of Icamentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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“…The unconventional yeast Y. lipolytica is a well-studied oleaginous model organism for investigating lipid production and accumulation in eukaryotic cells. However, it also possesses capability for production of polyols and organic acid [12–18]. Despite many studies on erythritol synthesis using Y. lipolytica , the metabolic pathway of this phenomenon has never been described.…”

Section: Introductionmentioning

confidence: 99%

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Mirończuk

Biegalska

Dobrowolski

2017

Biotechnol Biofuels

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BackgroundErythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry.ResultsIn this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479g encoding transketolase (TKL1), gene YALI0F15587g encoding transaldolase (TAL1), gene YALI0E22649g encoding glucose-6-phosphate dehydrogenase (ZWF1), and gene YALI0B15598g encoding 6-phosphogluconate dehydrogenase (GND1). Here, we show that the crucial gene for erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of erythritol over the control strain.ConclusionThis work presents the importance of the PPP in erythritol synthesis and the feasibility for economic production of erythritol from glycerol by the yeast Y. lipolytica.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0772-6) contains supplementary material, which is available to authorized users.

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“…This shift could be also triggered by addition of analogues of the iCA lyase reaction product in the culture medium, namely oxalic and itaconic acid. This was evidenced by Kamzolova et al (2016) for the strain VKM Y-2373 grown on ethanol as a main carbon source. In the control culture, they obtained a CA and iCA titer of 62 and 18.3 g/L, respectively, whereas in the presence of 40 mM itaconic acid, the corresponding titers were equal to 82.7 and 28 g/L, respectively [62].…”

Section: Iso-citric Acidmentioning

confidence: 82%

“…This was evidenced by Kamzolova et al (2016) for the strain VKM Y-2373 grown on ethanol as a main carbon source. In the control culture, they obtained a CA and iCA titer of 62 and 18.3 g/L, respectively, whereas in the presence of 40 mM itaconic acid, the corresponding titers were equal to 82.7 and 28 g/L, respectively [62]. A similar effect was observed for oxalic acid, however in a lesser extent (70.0 and 23 g/L, respectively).…”

Section: Iso-citric Acidmentioning

confidence: 82%

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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The effect of oxalic and itaconic acids on threo-Ds-isocitric acid production from rapeseed oil by Yarrowia lipolytica

Cited by 26 publications

References 30 publications

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

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