The effect of partial hepatectomy on the ribonucleic acid polymerase of rat liver

Busch, S.; Chambon, Pierre; Mandel, P.; Weill, J

doi:10.1016/0006-291x(62)90185-7

Cited by 69 publications

(8 citation statements)

References 7 publications

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“…x 20 RNA also is able to affect the assay system, although bacterial RNA appears to be less effective than RNA from animal sources.…”

mentioning

confidence: 93%

Aspects of Control of Protein Synthesis in Normal and Regenerating Rat Liver, I. A Cytoplasmic Rna-Containing Fraction That Stimulates Amino Acid Incorporation

Hoagland¹,

Askonas²

1963

Proc. Natl. Acad. Sci. U.S.A.

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The discovery of messenger RNA and the concept of control of protein synthesis by repression have laid the groundwork for understanding the chemistry of information transfer and its control in bacteria.1 2 The present work was initiated to determine whether such mechanisms operated in higher organisms and whether they could be studied in cell-free preparations.We chose to examine certain properties of the cell-free protein-synthesizing system of rat liver as an approach to these problems. The ability of the liver to regenerate in response to partial hepatectomy supplies a valuable comparative system: the heightened protein synthetic activity of the regenerating liver may be compared with that characteristic of the normal adult state with respect to the occurrence of messenger RNA and possible cytoplasmic and nuclear control mechanisms.The present paper is a more definitive account of work previously reported.3 A number of reports have since appeared of the occurrence in animal cells of a nuclear RNA fraction having turnover and sedimentation properties similar to those of bacterial messenger RNA (see Discussion). The studies recounted here indicate that liver cytoplasm contains a fraction having certain properties in common with messenger RNA including the ability to stimulate the in vitro amino acid incorporation system. The paper to follow presents evidence that microsomes from normal liver are intrinsically less responsive to the messenger-like fraction (and to other manipulations of their in vitro environment) than are those from regenerating liver. This difference in microsomal responsiveness appears to be due to the presence in the normal system of a dissociable inhibitor which renders microsomes from either normal or regenerating liver less active in vitro.Materials and Methods.-Female Sprague-Dawley rats (Charles River Breeding Company), weighing 250-300 gm, were used for the experiments. Partial hepatectomies, removing approximately 65 per cent of the liver, were performed according to the technique of Higgins and Anderson.4 The animals were killed by decapitation 20 to 24 hr later, and the livers were excised and immediately chilled. Normal rats of the same stock and weight were used in comparative studies. All animals were deprived of food for 20 to 24 hr before killing.Preparation of cytoplasmic fractions: The livers were homogenized in two volumes of a medium containing: tris (hydroxymethyl) amino methane (Tris) buffer (pH 7.3) 0.1 M MgCl2 0.005 M; sucrose 0.15 M; KCl 0.0025 M; and 3-mercaptoethanol 0.005 M. Microsomes (M) were pre-pared from a 15,000 gm supernatant fraction by centrifugation at 100,000 g for 1 hr. The microsomal supernatant fraction was then further centrifuged at 105,000 g in the Spinco No. 40 rotor for 12-13 hr. The resulting pellet, after suspension in medium, is referred to as the X fraction. The supernatant solution from this centrifugation was used to prepare a pH 5 fraction by diluting it with an equal volume of water, adding 1 N acetic acid to pH 5.2, collecting the resultan...

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“…x 20 RNA also is able to affect the assay system, although bacterial RNA appears to be less effective than RNA from animal sources.…”

mentioning

confidence: 93%

Aspects of Control of Protein Synthesis in Normal and Regenerating Rat Liver, I. A Cytoplasmic Rna-Containing Fraction That Stimulates Amino Acid Incorporation

Hoagland¹,

Askonas²

1963

Proc. Natl. Acad. Sci. U.S.A.

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“…(66). However, liver regeneration enhances the activity of RNA polymerase (67). Thyroidectomy has no effect on DPN pyrophosphorylase (69,70).…”

Section: The Chromatin Spacementioning

confidence: 98%

Enzymology of the Nucleus

Siebert¹,

Humphrey²

1965

Advances in Enzymology - And Related Areas of Molecular Biology

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“…Thus an increase in RNA polymerase is detectable by 6 hr. after the operation (Busch, Chambon, Mandel & Weill, 1962;Tsukada & Lieberman, 1964a), whilst increased DNA polymerase activity is only seen after 18 hr. (Giudice et al 1964).…”

Section: (E) the Eflects Of Hormones On Rna Polymerasementioning

confidence: 98%

“…Soon after partial hepatectomy of 67 % of the liver, an increased rate of RNA synthesis occurs in the remaining 33 % of the liver (Fujioka, Koga & Lieberman, 1963), and particularly in the nucleoli of the cells (Tsukada & Lieberman, 1964b). These rises in RNA synthesis are accompanied by increases in hepatic RNA polymerase activity (Busch et al 1962; Tsukada & Lieberman, 1965b), as measured in nuclear and nucleolar preparations (Tsukada & Lieberman, 1 9 6 4~) .…”

Section: (E) the Eflects Of Hormones On Rna Polymerasementioning

confidence: 99%

The Control of Protein Biosynthesis

Cohen

1966

Biological Reviews

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The effect of partial hepatectomy on the ribonucleic acid polymerase of rat liver

Cited by 69 publications

References 7 publications

Aspects of Control of Protein Synthesis in Normal and Regenerating Rat Liver, I. A Cytoplasmic Rna-Containing Fraction That Stimulates Amino Acid Incorporation

Aspects of Control of Protein Synthesis in Normal and Regenerating Rat Liver, I. A Cytoplasmic Rna-Containing Fraction That Stimulates Amino Acid Incorporation

Enzymology of the Nucleus

The Control of Protein Biosynthesis

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