The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles

Park, Margriet V. D. Z.; Neigh, Arianne M.; Vermeulen, Jolanda P.; Fonteyne, Liset J.J. de la; Verharen, Henny W.; Briedé, Jacob J.; Loveren, Henk Van; Jong, Wim H. de

doi:10.1016/j.biomaterials.2011.08.085

Cited by 918 publications

(647 citation statements)

References 41 publications

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“…Our results of Ag NPs toxicity are in qualitative agreement with the works of Park et al [32] and Carlson et al [33] who found a larger toxicity of small sizes NPs when expressed in concentration, but a lower toxicity when expressed in number. The values of IC50 obtained in these two references are however much larger, the CFE test being much more sensitive than the classical tests such as WST, LDH or MTT.…”

Section: Nanoparticlessupporting

confidence: 93%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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Section: Nanoparticlessupporting

confidence: 93%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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show abstract

“…their size, size distribution, shape, state of dispersion, physical and chemical properties, surface area and porosity, surface chemistry) and by cell characteristics (e.g. cell line, number of cells per well, and the period of culture before and/or after exposure to nanoparticles) 17,[24][25][26]29,30) . A recent study showed a decrease in MC3T3-E1 cell viability after exposure to Ag-NPs using the WST-8 assay 31) , but this study used different test conditions for cell culture and exposure to Ag-NPs, which may explain the disparity between their results and ours.…”

Section: Discussionmentioning

confidence: 99%

Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

et al. 2013

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With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new waterdispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured murine macrophages (RAW264.7) and osteoblastlike cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs.

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“…Nanoparticles <100 nm in diameter tend to interact with cellular organelles, including the mitochondria and nucleus, and these interactions can trigger cellular respiratory and gene toxicity in cells [20]. This risk is reduced with increasing NP size, presumably because larger NPs tend to initiate phagocytosis, which effectively isolates particles from the more sensitive cytoplasmic environment.…”

Section: Size and Shapementioning

confidence: 99%

“…Anionic NPs, by contrast, have been associated with little to no toxicity [20]. Furthermore, NPs with a charge below −30 mV have been found to have anti-inflammatory properties and when combined with antigen can induce antigen-specific immune tolerance [6,8,9].…”

Section: Chargementioning

confidence: 99%

Harnessing Nanoparticles for Immune Modulation

Getts¹,

Shea²,

Miller³

et al. 2016

Trends in Immunology

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Recent approaches using nanoparticles engineered for immune regulation have yielded promising results in preclinical models of disease. The number of nanoparticle therapies is growing, fueled by innovations in nanotechnology and advances in understanding of the underlying pathogenesis of immune-mediated diseases. In particular, recent mechanistic insight into the ways in which nanoparticles interact with the mononuclear phagocyte system and impact its function during homeostasis and inflammation have highlighted the potential of nanoparticle-based therapies for controlling severe inflammation while concurrently restoring peripheral immune tolerance in autoimmune disease. Here we review recent advances in nanoparticle-based approaches aimed at immune-modulation, and discuss these in the context of concepts in polymeric nanoparticle development, including particle modification, delivery and the factors associated with successful clinical deployment.

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The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles

Cited by 918 publications

References 41 publications

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

Harnessing Nanoparticles for Immune Modulation

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