The effect of PEGylated iron oxide nanoparticles on sheep ovarian tissue: An ex-vivo nanosafety study

Abstract

Today, nanotechnology plays an important role in our ever-continuous quest to improve the quality of human life. Because of their infinitesimal size, nanostructures can actively interact and alter cellular functions. Therefore, while the clinical benefits of nanotechnology may outweigh most of the associated risks, assessment of the cytotoxicity of nanostructures in respect to cells and tissues early in product development processes is of great significance. To the best of our knowledge, no such assessment has been performed for nanomaterials on the ovarian cortex before. Herein, silica-coated, PEGylated silica-coated, and uncoated iron oxide nanoparticles (IONP) with core diameter of 11 nm (AE4.2 nm) were synthesized. The oxidative stress in cultured ovarian tissue exposed to the various IONP was subsequently assessed. The results indicate that among the four groups, uncoated IONP induce the most oxidative stress on the ovarian cortex while tissues treated with PEGylated IONP exhibit no significant change in oxidative stress.

Nanowarming improves survival of vitrified ovarian tissue and follicular development in a sheep model

“…Finally, the particles were PEGylated in a PEG-silane (Merck, Germany) solution. After synthesis, the resulting IONP@Si@PEG were characterized with Dynamic Light Scattering (DLS), Zeta-potential (SZ-100 Nanoparticle Analyzer – Horiba, Japan), X-Ray Diffraction (XRD) (Philips PW 1730, Germany), Fourier Transform Infrared Spectroscopy (FTIR) (Thermo Nicolet AVATAR 370 FTIR) and Transmission Electron Microscopy (TEM) (Leo 912 Ab Omega, Zeiss) operated at 120 kV and the toxicity of IONP@Si@PEG evaluated in our pervious study [ 44 ].…”

Radioprotective Effect of Zinc Nanoparticles on Ionizing Radiation-induced Nephrotoxicity in Mice

“…SOD Assay : To measure SOD and CAT activity, the kidneys were weighed and homogenized in phosphate-buffered saline at 4°C and then centrifuged, and the supernatant was kept for assessments. The measurement of SOD activity was done by pyrogallol auto-oxidation effect and the absorbance was measured at 570 nm [42].…”

Benefits and challenges of nanomaterials in assisted reproductive technologies

“…For female gametes, after culturing early follicles enclosed in sheep ovarian tissues in vitro, Karimi et al (2020) showed that uncoated iron oxide nanoparticles induce oxidative stress on the ovarian cortex while tissues treated with PEGylated iron oxide nanoparticles exhibit no significant change in oxidative stress.…”

“…For female gametes, after culturing early follicles enclosed in sheep ovarian tissues in vitro , Karimi et al (2020) showed that uncoated iron oxide nanoparticles induce oxidative stress on the ovarian cortex while tissues treated with PEGylated iron oxide nanoparticles exhibit no significant change in oxidative stress. M. Ghorbani et al (2019) showed that green synthesized zinc oxide nanoparticles and commercial zinc oxide nanoparticles have negative effects on preantral follicles through increasing the production of free radicals, reducing the expression of estradiol and testosterone, reducing expression of GDF‐9 and BMP‐15, as well as increasing the expression of Foxo1 and VNN1.…”