The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

He, Qianjun; Zhang, Jiamin; Shi, Jianlin; Zhu, Ziyan; Zhang, Linxia; Bu, Wenbo; Guo, Limin; Chen, Yu

doi:10.1016/j.biomaterials.2009.10.046

Cited by 453 publications

(358 citation statements)

References 37 publications

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“…6,34 Haemolysis is defined as the destruction of red blood cells and it is regarded as a key parameter for the evaluation of NP biocompatibility. 6,34,35 NP can exert haemolytic effect by electrostatic interactions with membrane proteins or by other NP-specific mechanisms such as the generation of reactive oxygen species (ROS), causing irreversible damage to cells. The assessment of the haemolysis of uncoated BFO-NP showed a weak haemolytic potential (Figure 6), comparable to that of metallic NP observed in other bio-assays.…”

Section: Haemolysis Assaymentioning

confidence: 99%

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Staedler

Passemard

Magouroux

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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Bismuth Ferrite (BFO) nanoparticles (BFO-NP) display interesting optical (nonlinear response) and magnetic properties which make them amenable for bio-oriented diagnostic applications as intra-and extra membrane contrast agents. Due to the relatively recent availability of this material in well dispersed nanometric form, its biocompatibility was not known to date. In this study, we present a thorough assessment of the effects of in vitro exposure of human adenocarcinoma (A549), lung squamous carcinoma (NCI-H520), and acute monocytic leukemia (THP-1) cell lines to uncoated and poly(ethylene glycol)-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility. Our results support the attractiveness of the functional-BFO towards biomedical applications focused on advanced diagnostic imaging.

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Section: Haemolysis Assaymentioning

confidence: 99%

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Staedler

Passemard

Magouroux

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

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“…(i) it mostly requires tedious organic synthesis and surface modification 13 and (ii) pores of MSNs may be closed by the long PEG polymer chains, which can hinder the drug loading process. To overcome these limitations, here we report a facile self-assembly method using octyl modified hydrophobic MSNs and an amphiphilic block copolymer (F127 control experiments bare MSNs were synthesized without the addition of OTS (see ESI † for details).…”

mentioning

confidence: 99%

Pluronic polymer capped biocompatible mesoporous silica nanocarriers

et al. 2013

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A facile self-assembly method is described to prepare PEGylated silica nanocarriers using hydrophobic mesoporous silica nanoparticles and a pluronic F127 polymer. Pluronic capped nanocarriers revealed excellent dispersibility in biological media with cyto-and blood compatibilities.The high surface area and pore volume, good chemical stability and ease of surface functionalization of mesoporous silica nanoparticles (MSNs) make them promising materials for biological applications as drug carriers and theranostic agents.1,2 In addition silica based materials are generally accepted as biocompatible materials by the U.S. Food and Drug Administration (FDA). However, recent studies demonstrated their potential in vitro and in vivo toxicity, especially when their size is reduced to the nano scale. 3,4 Although the toxicity of silica based nanomaterials depends on several factors including particle size, shape, surface chemistry and porosity, [5][6][7] there is a general consensus that chemical structure of the surface is the predominant factor which determines the interactions with biological systems. 8 The surface of bare silica is covered with negatively charged silanol groups, which can electrostatically interact with positively charged tetraalkylammonium moieties of the cell membrane and can lead to cytotoxicity by membranolysis or inhibition of cellular respiration. 8,9 Also, rapid aggregation of silica based nanoparticles in biological media can result in mechanical obstruction in the capillary vessels of several vital organs, leading to organ failure and even death. 10,11 Therefore, replacing the surface silanol groups with biocompatible molecules is essential to improve the biocompatibility of MSNs. Among numerous polymeric or organosilane surface modification ligands, polyethylene glycol (PEG) is the mostly used one due to its well established biocompatibility, hydrophilicity, and antifouling properties.12 However, the PEGylation process has some limitations;(i) it mostly requires tedious organic synthesis and surface modification 13 and (ii) pores of MSNs may be closed by the long PEG polymer chains, which can hinder the drug loading process. To overcome these limitations, here we report a facile self-assembly method using octyl modified hydrophobic MSNs and an amphiphilic block copolymer (F127). F127, a FDA approved biocompatible pluronic polymer, contains two hydrophilic PEG blocks and a hydrophobic polypropylene oxide (PPO) between the two PEG blocks. 14 When the powder of hydrophobic MSNs is added into the F127 solution they are easily transferred into water by selfassembly of F127 molecules onto the MSN surface through the hydrophobic interaction between the PPO block of F127 and surface octyl groups of the MSNs (Scheme 1). In addition, cargo loaded and PEGylated MSNs can be simply prepared by loading the hydrophobic MSNs before the F127 capping process. The F127 capped particles are dispersible in both water and phosphate buffered saline (PBS), whereas uncapped MSNs are easily aggregated and precipitated...

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“…36 The hemolytic potential, defined as the lysis of red blood cells, is an adverse event which might occur upon exposure to NPs. 37 Uncoated USPIO NPs showed a high hemolytic potential, causing the lysis of 100% of human red blood cells after 2 h exposure (Fig. 2).…”

mentioning

confidence: 99%

Convenient synthesis of heterobifunctional poly(ethylene glycol) suitable for the functionalization of iron oxide nanoparticles for biomedical applications

Passemard

Staedler

Učňová

et al. 2013

Bioorganic & Medicinal Chemistry Letters

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The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

Cited by 453 publications

References 37 publications

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Pluronic polymer capped biocompatible mesoporous silica nanocarriers

Convenient synthesis of heterobifunctional poly(ethylene glycol) suitable for the functionalization of iron oxide nanoparticles for biomedical applications

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