The influence of genetic variability on the response of five cotton cultivars to Zn applied at 0 ppm (original Zn deficient soil), 5 ppm and 50 ppm was studied in a greenhouse experiment. Zinc content was determined in samples of shoots taken eight weeks after sowing and in samples of stock harvested at maturity. Typical zinc deficiency symptoms, varying in intensity among cultivars, were noted, which were characterized by interveinal chlorosis in the 3rd and 4th leaf and cupping.All the cultivars showed a response in yield to applied Zn both at 8 weeks after sowing and at maturity. At maturity, cultivars differed with respect to the magnitude of response of different plant parts to applied Zn. Some cultivars, recovered considerably from Zn stress conditions as plants matured. The lint yield of all the cultivars was highest with 50 ppm Zn. The yield of seed in 3 out of 5 cultivars was more with 50 ppm than with 5 ppm Zn. There was more difference among the cultivars in their shoot and stock yields than in the lint and seed yields. The effect of Zn deficiency was more pronounced on the lint and seed yields than on the stock yields.Zinc concentration in shoots at 8-weeks after sowing, and at maturity was not related to yield response.