The Effect of Photobiomodulation on Human Mesenchymal Cells: A Literature Review

Pinto, Hernán; Oliver, Paloma Goñi; Mengual, Elena Sánchez-Vizcaíno

doi:10.1007/s00266-021-02173-y

Cited by 18 publications

(7 citation statements)

References 62 publications

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“…Technical challenges in application of PBM remained to be addressed. Previous studies have focused on the evaluation of cellular responses based on wavelength variance or tried to determine cellular responses caused by diverse conditions with an identical type of light source [44][45][46]. Notably, contrasting results have been observed when different types of cells are irradiated with light of the same wavelength [47,48].…”

Section: Discussionmentioning

confidence: 99%

Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing

Lee

Kim

et al. 2022

Stem Cell Res Ther

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Background Human adipose-derived stem cells (hADSCs) have been widely used for regenerative medicine because of their therapeutic efficacy and differentiation capacity. However, there are still limitations to use them intactly due to some difficulties such as poor cell engraftment and viability after cell transplantation. Therefore, techniques such as photobiomodulation (PBM) are required to overcome these limitations. This study probed improved preclinical efficacy of irradiated hADSCs and its underlying molecular mechanism. Methods hADSCs were irradiated with green organic light-emitting diodes (OLEDs). Treated cells were analyzed for mechanism identification and tissue regeneration ability verification. Expression levels of genes and proteins associated with photoreceptor, cell proliferation, migration, adhesion, and wound healing were evaluated by performing multiple assays and immunostaining. Excision wound models were employed to test in vivo therapeutic effects. Results In vitro assessments showed that Opsin3 (OPN3) and OPN4 are both expressed in hADSCs. However, only OPN4 was stimulated by green OLED irradiation. Cell proliferation, migration, adhesion, and growth factor expression in treated hADSCs were enhanced compared to control group. Conditioned medium containing paracrine factors secreted from irradiated hADSCs increased proliferation of human dermal fibroblasts and normal human epidermal keratinocytes. Irradiated hADSCs exerted better wound healing efficacy in vivo than hADSCs without OLED irradiation. Conclusions Our study introduces an intracellular mechanism of PBM in hADSCs. Our results revealed that photoreceptor OPN4 known to activate Gq-protein and consequently lead to reactive oxygen species production responded to OLED irradiation with a wavelength peak of 532 nm. In conclusion, green OLED irradiation can promote wound healing capability of hADSCs, suggesting that green OLED has potential preclinical applications.

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Section: Discussionmentioning

confidence: 99%

Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing

Lee

Kim

et al. 2022

Stem Cell Res Ther

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“…Pinto et al 64 analyzed English articles on the PubMed database in human MSCs with in vitro PBM preconditioning. They highlighted the most suitable procedures and various parameter criteria, including wavelength (nm), irradiance (W/cm 2 ), dose/fluence (J/cm 2 ), power output (mW), duration of treatment (seconds), and frequency of treatment (number of times the treatment was carried out and the period).…”

Section: Scarcity In Standardization Of Parameters In Pbm Proceduresmentioning

confidence: 99%

“…They highlighted the most suitable procedures and various parameter criteria, including wavelength (nm), irradiance (W/cm 2 ), dose/fluence (J/cm 2 ), power output (mW), duration of treatment (seconds), and frequency of treatment (number of times the treatment was carried out and the period). 64 The studies assess at least one of the following variables: viability, proliferation, differentiation, or migration. However, the procedures used for these studies varied considerably in assessing the different MSC variables.…”

Section: Scarcity In Standardization Of Parameters In Pbm Proceduresmentioning

confidence: 99%

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Woo¹,

Abueva²

2022

Medical Lasers

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Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into many other cell types typically isolated from adult tissue sources. The therapeutic potential of MSCs is considerable in regenerative medicine and has advanced over the years. Consequently, methods and tools that improve the therapeutic efficacy of MSCs have also been devised and utilized. Recent strategies used to enhance the therapeutic efficacy of MSC include photobiomodulation (PBM), which can increase proliferation and enhance differentiation into target cell types. PBM is a non-thermal, non-invasive method that utilizes light from coherent or non-coherent sources to influence metabolic activity at the cellular level and has been found to increase cell proliferation and migration in vitro, in vivo, and in clinical studies. However, little is known of the mechanism responsible for the effects of PBM on MSCs or its potential as an adjunctive treatment, which obscures appreciation of its regenerative applications. This review summarizes reports on the effects of PBM on different MSCs and provides perspectives on the ability of PBM to improve the therapeutic efficacy of MSCs in regenerative medicine.

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“…7 Additionally, PBT has been shown to affect SCs' survival, differentiation, proliferation, or migration, improving their ability to recover. 8 Thus, the development of novel strategies to treat skin wounds (SWs) in regenerative medicine as well as the restoration of the function of damaged cells may benefit from the combination of PBT and SCs. Much of our understanding of the biological impacts of red and near-infrared light has changed in the 50 years since the American Society for Photobiology was founded.…”

Section: Introductionmentioning

confidence: 99%

Assessing the role of combination of stem cell and light‐based treatments on skin wound repair: A meta‐analysis

Zheng

Zhang

et al. 2023

International Wound Journal

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The meta‐analysis aims to evaluate and compare the impact of the combination of stem cells (SCs) and light‐based treatments (LBTs) on skin wound (SW) repair. Examinations comparing SCs to LBT with SCs for SW repair was among the meta‐analysis from various languages that met the inclusion criteria. Using continuous random‐effect models, the results of these investigations were examined, and the mean difference (MD) with 95% confidence intervals was computed (CIs). Seven examinations from 2012 to 2022 were recruited for the current analysis including 106 animals with SWs. Photobiomodulation therapy (PBT) plus SCs had a significantly higher wound closure rate (WCR) (MD, 9.08; 95% CI, 5.55–12.61, p < 0.001) compared to SCs in animals with SWs. However, no significant difference was found between PBT plus SCs and SCs on wound tensile strength (WTS) (MD, 2.01; 95% CI, −0.42 to 4.44, p = 0.10) in animals with SWs. The examined data revealed that PBT plus SCs had a significantly higher WCR, however, no significant difference was found in WTS compared to SCs in animals with SWs. Nevertheless, caution should be exercised while interacting with its values since all the chosen examinations were found with a low sample size and a low number of examinations were found for the comparisons studied for the meta‐analysis.

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The Effect of Photobiomodulation on Human Mesenchymal Cells: A Literature Review

Cited by 18 publications

References 62 publications

Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing

Enhancing therapeutic efficacy of human adipose-derived stem cells by modulating photoreceptor expression for advanced wound healing

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Assessing the role of combination of stem cell and light‐based treatments on skin wound repair: A meta‐analysis

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