The Effect of Physiologic and Pathologic Processes Upon Certain Histochemically Detectable Substances in the Gingiva

Turesky, Samuel; Glickman, Irving; Fisher, Beverly

doi:10.1902/jop.1959.30.2.116

Cited by 20 publications

(5 citation statements)

References 2 publications

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“…1). However, the presence and intensity of giycogen staining varied with the degree and type of keratinization (Table 2) in accordance with the observations of previous workers (1,16,21). Nonkeratinized and para- Table 3 Relation between amount of glycogen and degree of subepiihelial injlammation in 43 control specimens with nondyspiastic epithelium Amount of glycogen keratotic epithelium usually contained large amounts of glycogen.…”

Section: Resultssupporting

confidence: 89%

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Content and distribution of glycogen in oral epithelial dysplasia

Isacsson

Shear

1981

European J Oral Sciences

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Abstract— Forty‐four oral lesions with epithelial dysplasia and 25 other benign and malignant lesionsof the oarl mucosa were examined after ataining with hematoxylin‐eosin and diastase controlled PAS. The intensity of the PAS‐Positivity for glycogen, The grade of dysplasia, the type of keratinization and the degreeof subepithelial inflmmation were recored. Histologically normal epithelium at the margins of the lesions were used as controls. The presence and amount of glycogen in normal epithlium varied with the Form of keratinization in that non‐orparakeratinized epithelium was rich in glycogen where as there was a negative glycogen‐reaction in orthokeratinized epithelium. The most striking feature was and abrupt limitation of the glycogen at the junction between nondysplastic and dysplastic epithlium. The difference in the amount of glycogen in normal and dysplastic epithlium as assessed semiquantitativbely, was statistically significant. The diastase controlled PAS‐staining may therefore be a useful method of distinguishing dysplastic form nondysplastic epithlium in doubtjurl cases. Pseudoepitheliomatous hyperplastic epithelium covering granular cell myoblastoma did not contain any glycogen. Five of six squamous cell acrcinomas nand four verrucous carcinomas contained no demosstrable glycogen. Glycogen was present in the epithelium of the cases of lichen planus and “denture hyperplasia” investigated.

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Section: Resultssupporting

confidence: 89%

“…The retention of glycogen in the spinosum layer of oral mucosa is reported to be favored by a subepithelial inflammatory reaction (9,21,23). The present study does not show any correlation between the amount of glycogen and the subepithelial inflammatory reaction.…”

Section: Discussioncontrasting

confidence: 68%

Content and distribution of glycogen in oral epithelial dysplasia

Isacsson

Shear

1981

European J Oral Sciences

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“…These studies were predominantly descriptive morphological investigations. Although several authors (Turesky et al, 1959;Frank, 1964;Meyer & Gerson, 1964) attempted to provide quantitative data at the light-microscopic level, a quantitative electron-microscopic description of epithelial cytology is lacking.…”

Section: Introductionmentioning

confidence: 99%

Application of stereologic methods to stratified gingival epithelia*

Schroeder

Münzel-Pedrazzoli

1970

Journal of Microscopy

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SUMMARY Stereologic point‐counting procedures were applied to estimate quantitative tissue and cytoplasm parameters of the oral and the junctional epithelium of the human gingiva. Three gingival biopsies of female children, obtained and processed under standardized conditions, served (1) to determine the minimal sample size of cytoplasmic area required for estimating representative volumetric parameters in different strata of both epithelia, and (2) to compare average volume and surface density data derived from standardized samples in epithelial cross sections cut at two planes orientated perpendicular to each other. Sampling of electron micrographs, performed at two levels of magnifications according to a systematic stratified random sampling procedure consisted of recording field strips parallel to the epithelial or tooth surface within each of the various strata. The optimal sample size required varied for different organelles and epithelial strata. Minimal sample size of cytoplasmic area per biopsy were 300–440 μm2 for basal, and 450 μm2 for stratum spinosum cells of oral epithelium, and 130–185 μm2 for basal cells of the junctional epithelium. Average morphometric parameters for gross tissue components (cells, nuclei, cytoplasm, intercellular space) which resulted from analysing 4900 μm2 tissue area in each of the epithelia in each biopsy, were almost identical when determined in two different section planes. Volume and surface density data of cytoplasmic organelles (mitochondria, rough and smooth endoplasmic reticulum, cytoplasmic filaments) resulting from a sample of 280 μm2 cytoplasmic area per stratum and biopsy, revealed differences of varying magnitude, when determined in the two section planes. These differences were markedly smaller than those between comparable strata of both epithelia. Discussion of sampling procedures concluded that the type of sampling used for the present study is suitable for comparative morphometric studies.

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“…This was defined as the mean distance from the surface to the base of the epithelium at the tip of the connective tissue papilla adjacent to the rete pegs in 1. The mean of three randomly selected widths was used (Turesky, Glickman & Fisher 1959). Ten sets of measurements were taken from each specimen.…”

Section: Materials and Iviethodsmentioning

confidence: 99%

Oral mucosa of the vervet monkey

Fleisch¹,

Cleaton‐Jones²,

Austin³

1980

J of Periodontal Research

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Specimens of oral mucosa were taken from alveolar mucosa, attached gingiva, cheek, floor of mouth, hard palate, lip, soft palate and tongue from vervet monkeys (Cercopithecus aethiops), and prepared for histological and histometric examination. Masticatory mucosa showed an epithelial-connective tissue union comprised of prominent thin rete pegs and connective tissue papillae. Lining mucosa had broader rete pegs and short connective tissue papillae. Elastic fibres were seen in all tissues except hard palate and attached gingiva. Histometric examination showed considerable variation in epithelial width and cell densities between the various epithelia. Tongue had the widest epithelium and alveolar mucosa the narrowest. Cell density was greatest in alveolar mucosa and least in cheek and floor of the mouth epithelium.

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The Effect of Physiologic and Pathologic Processes Upon Certain Histochemically Detectable Substances in the Gingiva

Cited by 20 publications

References 2 publications

Content and distribution of glycogen in oral epithelial dysplasia

Content and distribution of glycogen in oral epithelial dysplasia

Application of stereologic methods to stratified gingival epithelia*

Oral mucosa of the vervet monkey

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