The Effect of Picloram on Somatic Embryogenesis of Different Explants of Strawberry (Fragaria ananassa Duch.)

Gerdakaneh, Mohammad; Zohori, Majid

doi:10.9734/bbj/2013/2370

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…In Panax vietnamensis, sucrose at 3 % level was most effective for somatic embryogenesis as compared to glucose and fructose (Nhut et al 2011). In the studies of Gerdakaneh et al (2009) also, sucrose was the best carbon source for induction and development of somatic embryos. Hong et al (2008) reported that in somatic embryogenesis of Oncidium, maltose was less effective as compared to sucrose …”

Section: Maturation and Germination Of Somatic Embryosmentioning

confidence: 99%

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Rizvi

Kukreja

Bisht

2012

Physiol Mol Biol Plants

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Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH 4 NO 3 , 10.72 mM KNO 3, 1.13 μM 2,4-dichlorophenoxyacetic acid, 7.38 μM 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5 %) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.

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Section: Maturation and Germination Of Somatic Embryosmentioning

confidence: 99%

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Rizvi

Kukreja

Bisht

2012

Physiol Mol Biol Plants

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“…Moderate frequency of embryogenesis was recorded when using picloram alone (medium Sta3). Similar results were obtained by Kordestanni and Karami ( 2008 ) and Gerdakaneh and Zohor ( 2013 ) who reported that the 2-mg/l picloram yielded the highest percentage of embryonic calli in strawberry leaves of cultivars (Camarosa and Selva) and (Kurdistan, Paros, and Camarosa), respectively. However, another reports recommended other hormones for strawberry callus induction, such as 2,4-D in combination with BA (Wang et al 1984 ); NAA (Biswas et al 2007 ) and TDZ (Husaini et al 2008 ).…”

Section: Discussionsupporting

confidence: 88%

“…However, Kordestanni and Karami ( 2008 ) found that the hormone free medium is the best for the embryo developments. Gerdakaneh and Zohor ( 2013 ) reported that 1-mg/l picloram was the best for yielding the highest number of cotyledonary-stage embryos.…”

Section: Discussionmentioning

confidence: 99%

Defense response enhancement in strawberry via elicitors

2016

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In this study, cell-suspension culture of strawberry (Fragaria × ananassa), cultivars Camarosa, and Sweet Charlie has been established. Embryogenic callus was induced by incubating the in vitro juvenile leaf explants on medium, containing 2-mg/l picloram at dark. Suspension culture was initiated from 4-week-old embryogenic calli in the liquid MS medium with 1-mg/l 2,4-D and 2-mg/l picloram. Suspension culture was maintained by sub-culturing each 3 weeks into a fresh medium. At week 9 after third sub-cultures, torpedo and cotyledonary embryo stages were observed. Embryos were then developed into shoots on medium 1 mg/l of each BA and IBA. Obtained shoots were successfully rooted on 1-mg/ml GA3, 0.5-mg/ml BA, and 1-mg/ml IBA. To enhance the resistance availability in strawberry plants, elicitation was applied by adding the JA and SA elicitors to the suspension culture with two doses (0.5 and 1 mM) individually and in combination, in addition to the fungal homogenate of Macrophomina phasiolena at concentration of 106 spor/ml. The fawrky-1-Camarosa gene, which has defense-related function, was detected in the different elicited strawberry tissues and isolated via RT-PCR. The isolated gene was submitted to GenBank with accession number (KX096885).

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“…High exogenous auxin levels in the maintenance medium interfere with the polar auxin gradient that is established during embryogenesis and prevent correct apical-basal embryo patterning [76][77][78]. Therefore, to enable further somatic embryo development in conifers, the PGRs initially added to the induction and proliferation media are replaced with mild auxin (indole-3-butyric acid (IBA)), abscisic acid (ABA), and polyethylene glycol (PEG) [74,[79][80][81].…”

Section: Somatic Embryo Productionmentioning

confidence: 99%

Different Roles of Auxins in Somatic Embryogenesis Efficiency in Two Picea Species

Hazubska-Przybył

Ratajczak

Obarska

et al. 2020

IJMS

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The effects of auxins 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (1-naphthaleneacetic acid) or picloram (4-amino-3,5,6-trichloropicolinic acid; 9 µM) and cytokinin BA (benzyloadenine; 4.5 µM) applied in the early stages of somatic embryogenesis (SE) on specific stages of SE in Picea abies and P. omorika were investigated. The highest SE initiation frequency was obtained after 2,4-D application in P. omorika (22.00%) and picloram application in P. abies (10.48%). NAA treatment significantly promoted embryogenic tissue (ET) proliferation in P. abies, while 2,4-D treatment reduced it. This reduction was related to the oxidative stress level, which was lower with the presence of NAA in the proliferation medium and higher with the presence of 2,4-D. The reduced oxidative stress level after NAA treatment suggests that hydrogen peroxide (H2O2) acts as a signalling molecule and promotes ET proliferation. NAA and picloram in the proliferation medium decreased the further production and maturation of P. omorika somatic embryos compared with that under 2,4-D. The quality of the germinated P. abies embryos and their development into plantlets depended on the auxin type and were the highest in NAA-originated embryos. These results show that different auxin types can generate different physiological responses in plant materials during SE in both spruce species.

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The Effect of Picloram on Somatic Embryogenesis of Different Explants of Strawberry (Fragaria ananassa Duch.)

Cited by 8 publications

References 29 publications

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

Defense response enhancement in strawberry via elicitors

Different Roles of Auxins in Somatic Embryogenesis Efficiency in Two Picea Species

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