The effect of picosecond laser pulses on redox-dependent processes in mice red blood cells studied in vivo

Voronova, O S; Генинг, Т. П.; Абакумова, Т. В.; Sysolyatin, Aleksey; Zolotovskiy, Igor O.; Антонеева, И. И.; Ostatochnikov, V. A.; Генинг, С. О.

doi:10.1117/12.2037877

Cited by 2 publications

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“…Activation of lipid peroxidation leads to profound disorders of the membrane structure, to the formation of lipid-lipid and lipid-protein intermolecular «cross-links», that results in the change of physical and chemical properties of lipid matrix, the passive ion membrane permeability is transformed. At present the relationship between deformability, mechanical persistency, red blood cell life time and lipid peroxidation intensity has been proved [10].…”

Section: Introductionmentioning

confidence: 99%

Use of Atomic Force Microscopy for Assessing Paraneoplastic Changes in Red Blood Cells in Carcinogenesis Dynamics

Tp¹,

AYu²

2016

Transl Biomed

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Background: Thus, the main advantages of AFM in the study of biological objects are a high resolution, ease of sample preparation, determination of morphometric parameters, the study of objects in a native environment for them (in solution), the study of viscoelastic, charge, hydrophobic properties of objects. Methods and findings:In order to evaluate the possibility of atomic force microscopy use in the evaluation of paraneoplastic changes in erythrocytes of albino rats in stationary and terminal phases of experimental ovarian cancer, we biochemically determined the levels of lipid peroxidation products (malondialdehyde, ketodienes, diene conjugates and Schiff bases), the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione-S-transferase), and the levels of oxidized and reduced glutathione. Cytoarchitectonics, cross section and rigidity of red blood cells were assessed by atomic force microscopy. The reliability of the correlation between values was assessed with Spearman method. We found increased level of malondialdehyde, diene conjugates and ketodienes and increase in the activity of antioxidant enzymes-superoxide dismutase, catalase and glutathione-S-transferase simultaneously with reduction of reduced glutathione level and ratio of reduced and oxidized glutathione in erythrocytes during experimental ovarian cancer progression. At the same time there has been a significant increase in the indexes of reversible and irreversible transformation and rigidity of the membranes of red blood cells, which correlated with indicators of lipid peroxidation and antioxidants.Conclusions: Thus, the use of atomic force microscopy makes it possible to adequately assess paraneoplastic changes in red blood cells in the dynamics of the progression of experimental ovarian tumors.

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Section: Introductionmentioning

confidence: 99%