The effect of Platelet-Rich Plasma and Stromal Vascular Fraction combination on Epidermal Growth Factor serum level for anal trauma healing in the Wistar rat model

Abstract

Introduction Stromal Vascular Fraction cells (SVFs) and Platelet Rich Plasma (PRP) are clinically proven to aid in cell regeneration and wound healing. The healing effects can be measured by the level of Epidermal Growth Factor (EGF). This study aims to investigate the effect of an SVFs and PRP combination on EGF levels in the anal trauma model. Method Twenty-eight adult Wistar rats were divided into 3 groups: Group A consisted of healthy rats as a normal control group; Group B and C underwent modified anal surgical trauma and repair. Group B was treated with saline only and Group C was treated with local injection of a combination of SVFs and PRP after anal surgical repair. The EGF level was subsequently assessed on days 1, 7, and 14. Results EGF levels were generally increased in Group C compared to Group B. A one-way ANOVA test result showed significantly increased EGF levels on days 7 (p = 0.038) and 14 (p = 0.018). Based on the linear regression test results, we found that local injection of PRP and SVFs after anal repair on an anal surgical trauma model can increase the EGF level in group C by 36.9% more than that of group B. Conclusion The combination of PRP and SVFs can increase the EGF level in the wound healing process of anal trauma. EGF is critical in the anal trauma healing process.

Effects of Stromal Vascular Fraction and Platelet-Rich Plasma Treatment on Capillary Histopathology During Anal Trauma Healing in Rats: An in Vivo Study

“…Previous studies have suggested that wound healing will be enhanced by PRP and SVF treatment. 12,14 While the mechanism is not fully understood, its potential components and…”

“…The anal trauma model used in this study was adapted from Trebol et al 12,14,16 Sacrificing Procedure Rats from Groups A and B were sacrificed on days 1, 7, and 14 post-treament. All rats were anaesthetized with ether before sacrificed.…”

“…The number of rats in each subgroup was four based on Federer's formula. [12][13][14] Twenty-eight rats were divided into three groups. Group A (n=12) was treated with PRP and SVFs (treatment).…”

The role of diphenhydramine HCl on tumor necrosis factor-α levels in wistar rats with traumatic brain injury: An in vivo study

“…The total number of experimental rats required was 10, based on the Federer formula [ 8 ]. The criteria for inclusion were (1) rats (Rattus Norvegicus) of the Wistar male strain, (2) aged 2–3 months, (3) weighing 150–200 g, and (4) in good health.…”

The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model

“…Previously, Sirowanto et al found that local injection of an SVF and PRP combination within an anal trauma model increased the epidermal growth factor (EGF) level during the healing process [ 22 ]. In this study, we aim to determine the effect of an SVF and PRP combination on bFGF levels during anal trauma healing.…”