The Effect of Platelet-Rich Plasma on Muscle Contusion Healing in a Rat Model

Delos, Demetris; Leineweber, Matthew J.; Chaudhury, Salma; Alzoobaee, Saif; Gao, Yingxin; Rodeo, Scott A.

doi:10.1177/0363546514540272

Cited by 53 publications

(56 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Platelet poor plasma (PPP) and platelet rich plasma (PRP) were prepared from whole blood under sterile conditions. As no widely used, commercially available clinical system is available to prepare PRP from rats, a manual preparation approach modified from previous studies 1,11 was used. Briefly, blood was centrifuged at 500 × g for 5 minutes at 4°C, followed by a 5 minute rest period, and then another cycle again at 700 × g for 17 minutes at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Platelet-Rich Plasma Activates Proinflammatory Signaling Pathways and Induces Oxidative Stress in Tendon Fibroblasts

et al. 2016

View full text Add to dashboard Cite

Background Tendon injuries are one of the most common musculoskeletal conditions in active patients. Platelet rich plasma (PRP) has shown some promise in the treatment of tendon disorders, but little is known as to the mechanisms by which PRP can improve tendon regeneration. PRP contains numerous different growth factors and cytokines which activate various cellular signaling cascades, but it has been difficult to determine precisely which signaling pathways and cellular responses are activated following PRP treatment. Additionally, macrophages play an important role in modulating tendon regeneration, but the influence of PRP on determining whether macrophages assume a proinflammatory or antiinflammatory phenotype remains unknown. Hypothesis/Purpose We used genome wide expression profiling, bioinformatics and protein analysis to determine the cellular pathways activated in fibroblasts treated with PRP. We also evaluated the effect of PRP on macrophage polarization. Study Design Controlled laboratory study. Methods Tendon fibroblasts or macrophages from rats were cultured and treated with either platelet poor plasma (PPP) or PRP. RNA or protein was isolated from cells and analyzed using microarrays, qPCR, immunoblot or bioinformatics techniques. Results Pathway analysis determined that the most highly induced signaling pathways in PRP treated tendon fibroblasts was the TNFα and NFκB pathways. PRP also downregulated the expression of ECM genes and induced the expression of autophagy related genes and reactive oxygen species (ROS) genes and protein markers in tendon fibroblasts. PRP failed to have a major impact on markers of macrophage polarization. Conclusions PRP induces an inflammatory response in tendon fibroblasts which leads to the formation of ROS and activation of oxidative stress pathways. PRP does not appear to significantly modulate macrophage polarization. Clinical Relevance PRP might act by inducing a transient inflammatory event which could then trigger a tissue regeneration response.

show abstract

Section: Methodsmentioning

confidence: 99%

Platelet-Rich Plasma Activates Proinflammatory Signaling Pathways and Induces Oxidative Stress in Tendon Fibroblasts

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Clinical studies have shown variable results, 2,16,18,28,30 and basic science and animal studies range from positive outcomes 11,20,25,35 to suggesting little benefit for some types of injury. For example, a recent article by Delos et al 8 showed no benefit of using PRP in an animal model of contusion injury. There are many potential causes for the variation, such as the amount of PRP, concentration of PRP, and timing of PRP administration.…”

Section: Discussionmentioning

confidence: 99%

Site-specific Targeting Platelet-rich Plasma Via Superparamagnetic Nanoparticles

Lovering¹,

Vanegas²,

Pratt³

et al. 2014

Medicine & Science in Sports & Exercise

View full text Add to dashboard Cite

Background: Muscle strains are one of the most common injuries treated by physicians. Standard conservative therapy for acute muscle strains usually involves short-term rest, ice, and nonsteroidal anti-inflammatory medications, but there is no clear consensus regarding treatments to accelerate recovery. Recently, clinical use of platelet-rich plasma (PRP) has gained momentum as an option for therapy and is appealing for many reasons, most notably because it provides growth factors in physiological proportions and it is autologous, safe, easily accessible, and potentially beneficial. Local delivery of PRP to injured muscles can hasten recovery of function. However, specific targeting of PRP to sites of tissue damage in vivo is a major challenge that can limit its efficacy.

show abstract

“…Several studies have investigated the effect of a series of PRP injections on isometric torque muscle strength in rats. Delos et al found no difference in muscle strength at any time point and no difference in the number centronucleated fibers or inflammatory cells compared to control rodents injected with saline [42]. Hammond et al found no difference in strength for single muscle contraction injuries treated with PRP [43].…”

Section: Treatments To Enhance Return To Sports After Muscle Injurymentioning

confidence: 99%

Return to sport after muscle injury

Wong

Ning

Lee

2015

Curr Rev Musculoskelet Med

View full text Add to dashboard Cite

Skeletal muscle injuries are among the most common sports-related injuries that result in time lost from practice and competition. The cellular response to muscle injury can often result in changes made to the muscle fibers as well as the surrounding extracellular matrix during repair. This can negatively affect the force and range of the injured muscle even after the patient's return to play. Diagnosis of skeletal muscle injury involves both history and physical examinations; imaging modalities including ultrasound and magnetic resonance imaging (MRI) can also be used to assess the extent of injury. Current research is investigating potential methods, including clinical factors and MRI, by which to predict a patient's return to sports. Overall, function of acutely injured muscles seems to improve with time. Current treatment methods for skeletal muscle injuries include injections of steroids, anesthetics, and platelet-rich plasma (PRP). Other proposed methods involve inhibitors of key players in fibrotic pathways, such as transforming growth factor (TGF)-ß and angiotensin II, as well as muscle-derived stem cells.

show abstract

The Effect of Platelet-Rich Plasma on Muscle Contusion Healing in a Rat Model

Abstract: Before PRP can be recommended for the treatment of muscle contusion injuries, further translational and clinical investigations need to be performed.

Cited by 53 publications

References 27 publications

Platelet-Rich Plasma Activates Proinflammatory Signaling Pathways and Induces Oxidative Stress in Tendon Fibroblasts

Platelet-Rich Plasma Activates Proinflammatory Signaling Pathways and Induces Oxidative Stress in Tendon Fibroblasts

Site-specific Targeting Platelet-rich Plasma Via Superparamagnetic Nanoparticles

Return to sport after muscle injury

Contact Info

Product

Resources

About