“…Utilizing a 1064 nm Nd:YAG laser, we pursue the synthesis of CDs, delving into their optical characteristics, morphology, and size (Kaczmarek et al, 2021;Reyes et al, 2016). We meticulously quantify the energy absorption of CDs pre and post laser ablation, and upon integration with PEG400, adopting ImageJ for fluorescence intensity analysis in zebrafish ocular regions (Aji et al, 2017;Putro et al, 2019;Gao et al, 2020). Employing the Corrected Total Cell Fluorescence (CTCF) technique, we assess the fluorescence density across multiple image slices acquired via CLSM (Jakic et al, 2017), thereby enhancing our understanding of CDs' potential in bioimaging.…”