The Effect of Polyhydroxy Fullerene Derivative on Human Myeloid Leukemia K562 Cells

Guo, Wei; Liu, Xing; Ye, Lianjie; Liu, Jie; Kollie, Larwubah; Meng, Guang; Shen, Weiqiang; Ying, Xiangxian; Zhu, Jun; Yang, Shengjie; Guo, Jianjun; Jia, Yanrong; Yu, Meilan

doi:10.3390/ma15041349

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…However, it can increase with the functionalization of nanoparticles, which is necessary to increase biocompatibility [17]. At the same time, some publications [79,80] assert that C60 can be toxic to tumor cells. We analyzed the cytotoxicity of The detected photodynamic activity of the FA-PVP-C 60 conjugate is consistent with data obtained previously both for C 60 fullerene and for its conjugates with polymers [76,77].…”

Section: Toxicity Assaymentioning

confidence: 99%

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Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

Borisenkova,

Bolshakova,

Titova

et al. 2024

IJMS

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The use of targeted drug delivery systems, including those based on selective absorption by certain receptors on the surface of the target cell, can lead to a decrease in the minimum effective dose and the accompanying toxicity of the drug, as well as an increase in therapeutic efficacy. A fullerene C60 conjugate (FA-PVP-C60) with polyvinylpyrrolidone (PVP) as a biocompatible spacer and folic acid (FA) as a targeting ligand for tumor cells with increased expression of folate receptors (FR) was obtained. Using 13C NMR spectroscopy, FT-IR, UV-Vis spectrometry, fluorometry and thermal analysis, the formation of the conjugate was confirmed and the nature of the binding of its components was established. The average particle sizes of the conjugate in aqueous solutions and cell culture medium were determined using dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The FA-PVP-C60 showed antiradical activity against •DPPH, •OH and O2•−, but at the same time, it was shown to generate 1O2. It was found that the conjugate in the studied concentration range (up to 200 μg/mL) is non-toxic in vitro and does not affect the cell cycle. To confirm the ability of the conjugate to selectively accumulate through folate-mediated endocytosis, its uptake into cells was analyzed by flow cytometry and confocal microscopy. It was shown that the conjugate is less absorbed by A549 cells with low FR expression than by HeLa, which has a high level of expression of this receptor.

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Section: Toxicity Assaymentioning

confidence: 99%

“…However, it can increase with the functionalization of nanoparticles, which is necessary to increase biocompatibility [17]. At the same time, some publications [79,80] assert that C 60 can be toxic to tumor cells. We analyzed the cytotoxicity of the conjugate containing C 60 in comparison with the FA-PVP conjugate on several cell lines.…”

Section: Toxicity Assaymentioning

confidence: 99%

Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

Borisenkova,

Bolshakova,

Titova

et al. 2024

IJMS

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“…When given a high dose of amphiphilic polymer which was conjugated and self-assembled into nanoparticles, its half-life increased by approximately 20 fold and it was effective in inhibiting Bcr-Abl [ 76 ]. When K562 cells were administered with 160 μM of hydroxylated fullerene, it showed a decrease in the mitochondrial membrane potential, a change in morphology, and the G2-phase arrest [ 77 ]. CML stem cells aberrantly express CD26.…”

Section: Organic Nanoparticles In CML Therapymentioning

confidence: 99%

Supremacy of nanoparticles in the therapy of chronic myelogenous leukemia

Janani,

Girigoswami,

Girigoswami

2023

ADMET DMPK

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Background and purpose: The reciprocal translocation of the ABL gene from chromosome 9 to chromosome 22 near the BCR gene gives rise to chronic myelogenous leukemia (CML). The translocation results in forming the Philadelphia chromosome (BCR-ABL) tyrosine kinase. CML results in an increase in the number of white blood cells and alteration in tyrosine kinase expression. CML prognosis includes three stages, namely chronic, accelerated, and blast. The diagnosis method involves a CT scan, biopsy, and complete blood count. However, due to certain disadvantages, early diagnosis of CML is not possible by traditional methods. Nanotechnology offers many advantages in diagnosing and treating cancer. Experimental approach: We searched PubMed, Scopus and Google Scholar using the keywords Philadelphia chromosome, bionanotechnology, tyrosine kinase pathway, half-life, passive targeting, and organic and inorganic nanoparticles. The relevant papers and the classical papers in this field were selected to write about in this review. Key results: The sensitivity and specificity of an assay can be improved by nanoparticles. Utilizing this property, peptides, antibodies, aptamers, etc., in the form of nanoparticles, can be used to detect cancer at a much earlier stage. The half-life of the drug is also increased by nanoformulation. The nanoparticle-coated drugs can easily escape from the immune system. Conclusion: Depending on their type, nanoparticles can be categorized into organic, inorganic and hybrid. Each type has its advantages. Organic nanoparticles have good biocompatibility, inorganic nanoparticles increase the half-life of the drugs. In this review, we highlight the nanoparticles involved in treating CML.

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Fullerenol as a novel therapeutic agent for sepsis-induced cardiomyocytes damage

Zhang,

Chen,

Ding

et al. 2024

Appl. Phys. A

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Sepsis-induced myocardial damage (SIMD) is a serious complication of sepsis that affects cardiac function and prognosis. However, effective methods for the prevention and treatment of SIMD are presently lacking. This study reports the crucial function of fullerenols in protecting cardiomyocytes from septic injury. First, we applied the as-prepared fullerenols as an effective free radical scavenger and broad-spectrum anti-oxidant to protect against LPS-induced cardiomyocyte injury. Next, we verified their ability to downregulate IL-6, TNF-α, and ROS-dependent pyroptosis in AC16 induced by LPS. Finally, we observed that the protective mechanism of fullerenol against SIMD involves the regulation of the NFκB/iNOS/COX-2 pathway. This study presents a timely and essential investigation of the prevention and treatment of SIMD, providing new insights into the applications of fullerenols for therapeutic strategies.

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The Effect of Polyhydroxy Fullerene Derivative on Human Myeloid Leukemia K562 Cells

Cited by 6 publications

References 36 publications

Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

Supremacy of nanoparticles in the therapy of chronic myelogenous leukemia

Fullerenol as a novel therapeutic agent for sepsis-induced cardiomyocytes damage

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