The effect of pre-enrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture

Myint, Maung San; Johnson, Yvette J.; Tablante, Nathaniel L.; Heckert, Robert A.

doi:10.1016/j.fm.2005.09.002

Cited by 116 publications

(77 citation statements)

References 23 publications

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“…Salmonella spp. reported that there are in poultry gut 10 and support the results observed in the present study. Meanwhile, obtained values and percentages for naturally occurring Salmonella spp.…”

Section: Discussionsupporting

confidence: 93%

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Mirhosseini¹,

Seidavi²,

Saifeddine³

et al. 2009

Kafkas Univ Vet Fak Derg

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Section: Discussionsupporting

confidence: 93%

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Mirhosseini¹,

Seidavi²,

Saifeddine³

et al. 2009

Kafkas Univ Vet Fak Derg

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“…However, a positive rate of 90% was observed after enrichment. Generally, culture enrichment is recommended in order to distinguish live cells from dead cells before PCR [45]. Maciorowski et al investigated diferent enrichment times to detect indigenous Salmonella in poultry dietary samples using PCR.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Current and Emerging Innovations for Detection of Food-Borne Salmonella

Wu¹,

Zeng²

2017

Current Topics in Salmonella and Salmonellosis

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Salmonella is one of the leading causes of food-borne illnesses worldwide, and one of the main contributors to salmonellosis is the consumption of contaminated egg, poultry, pork, beef, and milk products. Since deleterious efects of Salmonella on public health and the economy continue to occur, improving safety of food products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Therefore, there is an ongoing need to develop more advanced detection methods that can identify Salmonella accurately and rapidly in foods before they reach consumers. In the past three decades, there have been increasing eforts toward developing and improving rapid pathogen detection and characterization methodologies for application to food products. In this chapter, we discuss molecular methods for detection, identiication, and genetic characterization of Salmonella in food. In addition, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here. The methods with potential application to the industry are highlighted in this chapter.

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“…Nucleic acids extractions techniques may require optimisation [46] and PCR assays with environmental and bioengineered samples are often particularly challenging [47]. The sensitivity and specificity of qPCR assays, and of the Nucleic Acids diagnostic Tests (NATs) developed thereof, are critical considerations [48]. The cost and reproducibility, as well as the ease-ofuse and robustness, of NATs for different samples is also important for optimal use of quantitative, molecular diagnostics tools to monitor the metals-related 'health' of functional groups in anaerobic biotechnologies.…”

Section: Trace Metal Microbiology and Microbial Ecologymentioning

confidence: 99%

Fate of Trace Metals in Anaerobic Digestion

Fermoso

Hullebusch²,

Guibaud

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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A challenging, and largely uncharted, area of research in the field of anaerobic digestion science and technology is in understanding the roles of trace metals in enabling biogas production. This is a major knowledge gap and a multifaceted problem involving metal chemistry; physical interactions of metal and solids; microbiology; and technology optimization. Moreover, the fate of trace metals, and the chemical speciation and transport of trace metals in environmentsoften agricultural lands receiving discharge waters from anaerobic digestion processes -is completely unknown but simultaneously represents challenges for environmental protection and opportunities to close process loops in anaerobic biotechnologies. Affiliation 1.

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The effect of pre-enrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture

Cited by 116 publications

References 23 publications

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Broyler Piliçlerin Gastrointestinal Sistemindeki Salmonella spp’nin Polimeraz Zincir Reaksiyonu ile Belirlenmesi

Current and Emerging Innovations for Detection of Food-Borne Salmonella

Fate of Trace Metals in Anaerobic Digestion

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