The effect of pretreatment or combined treatment of quercetin on menadione toxicity in rat primary mixed glial cells in vitro

Abstract

Neurons and glia are highly susceptible to reactive oxygen species that play a key role in various neurodegenerative diseases. Menadione, a synthetic derivative of vitamin K, induces reactive oxygen generation. Quercetin one of the most ubiquitous bioflavonoids in food of plant origin, has strong antioxidant activities on different cell types, however recent studies demonstrated that it has also prooxidant and cytotoxic potentials. We examined the action of pre- and co-treatment of quercetin on menadione induced glial toxicity. The primary mixed glial cells obtained from 1 to 3 day old rat brain were pretreated with 10, 25, 100 or 250 muM quercetin for 1 h, washed out and 10, 25, 50, 75 or 100 muM menadione was added for 6 h. The other group of cells was treated with respective doses of quercetin combined simultaneously with the same doses of menadione for 6 h. The cells were washed and incubated for additional 24 h for recovery period and the viability was measured by using MTT assay. Menadione was dose-dependently toxic to glia cells and pretreatment with respective quercetin doses for 1 h could not eliminate this toxicity. Although 10 and 25 muM quercetin combined with 10 and 25 muM menadione could not change, 100 and 250 muM quercetin together with 10 or 25 muM menadione for 6 h increased further the menadione induced toxicity. We conclude that when combined with menadione, quercetin at high doses could be toxic to primary rat glia cells in culture.

Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes

“…Recent studies have indicated that the oxidative stress develops when the levels of antioxidants are lowered (Tapiero et al 2004;Banudevi al. 2005;Oztopcu-Vatan et al 2009). Individual antioxidants can be considered very sensitive in revealing a pro-oxidant challenge (Regoli and Winston 1999).…”

Quercetin’in HepG2 hücrelerinde, TNF-Alfa ve LPS ile indüklenen enflamasyonda, eritropoietin, HIF-1α, HIF-2α ve piroptoz ile ilgili genlerin ekspresyon seviyeleri üzerine etkileri

“…A study in HepG2 cells reported that quercetin might exert dose-dependent cytoprotective and cytotoxic effects 7 . It has been reported that low concentrations and short-term administration of quercetin have antioxidant and anti-apoptotic effects, and at high concentrations, it disrupts the antioxidant defense system, causing oxidative damage and increasing apoptosis [31][32][33] . As a result, the effect of quercetin may vary according to concentration, application time, applied cell type or culture conditions, and contradictory effects may be seen at the same time.…”