The effect of progesterone on systemic inflammation and oxidative stress in the rat model of sepsis

Aksoy, Ayşe; Toker, Aysun; Çelik, Mine; Aksoy, Mehmet; Halıcı, Zekai; Aksoy, Hülya

doi:10.4103/0253-7613.144922

Cited by 44 publications

(30 citation statements)

References 28 publications

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“…However, when progesterone was also administered, the TH + neurone density was not significantly different from the controls, nor from the MPTP‐treated mice. There is evidence regarding the beneficial effects of progesterone on inflammation . Hence, we next investigated the effect of progesterone treatment on pro‐inflammatory macrophages in the myenteric plexus.…”

Section: Resultsmentioning

confidence: 99%

Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease

Jarras

Bourque

Poirier

et al. 2019

J Neuroendocrinology

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Gastrointestinal symptoms appear in Parkinson's disease patients many years before motor symptoms, suggesting the implication of dopaminergic neurones of the gut myenteric plexus. Inflammation is also known to be increased in PD. We previously reported neuroprotection with progesterone in the brain of mice lesioned with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) and hypothesised that it also has neuroprotective and immunomodulatory activities in the gut. To test this hypothesis, we investigated progesterone administered to adult male C57BL/6 mice for 10 days and treated with MPTP on day 5. In an additional experiment, progesterone was administered for 5 days following MPTP treatment. Ilea were collected on day 10 of treatment and microdissected to isolate the myenteric plexus. Dopaminergic neurones were reduced by approximately 60% and pro‐inflammatory macrophages were increased by approximately 50% in MPTP mice compared to intact controls. These changes were completely prevented by progesterone administered before and after MPTP treatment and were normalised by 8 mg kg‐1 progesterone administered after MPTP. In the brain of MPTP mice, brain‐derived neurotrophic peptide (BDNF) and glial fibrillary acidic protein (GFAP) were associated with progesterone neuroprotection. In the myenteric plexus, increased BDNF levels compared to controls were measured in MPTP mice treated with 8 mg kg‐1 progesterone started post MPTP, whereas GFAP levels remained unchanged. In conclusion, the results obtained in the present study show neuroprotective and anti‐inflammatory effects of progesterone in the myenteric plexus of MPTP mice that are similar to our previous findings in the brain. Progesterone is non‐feminising and could be used for both men and women in the pre‐symptomatic stages of the disease.

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Section: Resultsmentioning

confidence: 99%

Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease

Jarras

Bourque

Poirier

et al. 2019

J Neuroendocrinology

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“…It is well-known that interleukin 6 plays an important role in diagnosis and prediction of mortality of patients with sepsis [18,19]. Most sepsis studies are based on serum interleukin 6 levels.…”

Section: Discussionmentioning

confidence: 99%

“…Also, they reported that interleukin 6 levels in brain tissue are not significantly changed after 24 h. In the literature, TNF-alpha was commonly measured as a marker for sepsis associated encephalopathy in the brain tissue. The interleukin 6 levels were measured in serum [1,19,21–24]. …”

Section: Discussionmentioning

confidence: 99%

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Arslan

Ekinci

Arıcı

et al. 2017

Libyan Journal of Medicine

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Despite recent advances in antibiotic therapy, sepsis remains a major clinical challenge in intensive care units. Here we examined the anti-inflammatory and antioxidant effects of Ecballium elaterium (EE) on brain, and explored its therapeutic potential in an animal model of sepsis-associated encephalopathy (SAE) [induced by cecal ligation and puncture (CLP)].Thirty rats were divided into three groups of 10 each: control, sepsis, and treatment. Rats were subjected to CLP except for the control group, which underwent laparatomy only. The treatment group received 2.5 mg/kg EE while the sepsis group was administered by saline. Twenty-four hours after laparotomy, animals were sacrificied and the brains were removed. Brain homogenates were prepared to assess interleukin 1beta (IL-1β), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), total antioxidant capacity (TAC), and total oxidant status (TOS). Brain tissue sections were stained by hematoxylin and eosin (H&E) to semi-quantitatively examine the histopathologic changes such as neuron degeneration, pericellular/perivascular edema and inflammatory cell infiltration in the cerebral cortex.We found a statistically significant reduction in brain tissue homogenate levels of TNF-α 59.5 ± 8.4/50.2 ± 6.2 (p = 0.007) and TOS 99.3 ± 16.9/82.3 ± 7.8 (p = 0.01) in rats treated with EE; although interleukin 6 levels were increased in the treatment group compared to the sepsis group, this was not statistically significant. Neuronal damage (p = 0.00), pericellular/perivascular edema and inflammatory cell infiltration (p = 0.001) were also significantly lower in the treatment group compared to those in the sepsis group.These data suggest that Ecballium elaterium contains some components that exert protective effects against SAE in part by attenuating accumulation of proinflammatory cytokines, which may be important contributors to its anti-inflammatory effects during sepsis.

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“…[25] Other research indicated that there was an increase in kidney MPO values as well as MDA values in kidney and liver tissue, but there was no significant different in liver MPO level. [41] The effect of long-term use of dapagliflozin on glucose homeostasis and diabetic nephropathy has previously been investigated. Dapagliflozin has improved hyperglycemia and albuminuria, depending on the dose (0.1-1.0 mg/kg), and reduced macrophage infiltration, gene expression of inflammatory cytokines, and oxidative stress in diabetic mice.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dapagliflozin on Experimental Sepsis Model in Rats

Kıngır¹

2018

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: The aim of this study was to evaluate the possible protective effects of dapagliflozin in an experimental sepsis model in rats. METHODS: Saline (1 mL/kg, p.o.) or dapagliflozin (10 mg/kg, p.o.) was administered to Sprague-Dawley rats for 5 days prior to the surgical procedures. Under anesthesia, sepsis was induced by cecal ligation puncture, while sham control groups underwent laparotomy only. Blood urea nitrogen, creatinine, and glucose levels were measured in serum samples and the levels of malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO), tumor necrosis factor alpha, interleukin 1 beta, caspase 8, and caspase 9 were determined in tissue samples (kidney, liver, and lung). Histological evaluation was also performed. RESULTS: The administration of dapagliflozin in a sepsis model reduced oxidative stress (MDA), increased antioxidant levels (GSH), and reduced inflammation (MPO) in the kidney (p<0.05). Dapagliflozin also decreased oxidative stress (MDA) in lung tissue and decreased inflammation (MPO) in lung and liver tissue (p<0.05). Caspase 8 and 9 levels in kidney, lung, and liver tissue were increased (p<0.05) in the dapagliflozin group compared with the sepsis group. According to the histopathological results, sepsis was moderately improved in renal tissue and slightly attenuated in lung and liver tissue with the administration of dapagliflozin. CONCLUSION: Dapagliflozin had a preventive effect on sepsis-induced kidney damage, but the protective effect was mild in lung and liver tissue in the present study.

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The effect of progesterone on systemic inflammation and oxidative stress in the rat model of sepsis

Cited by 44 publications

References 28 publications

Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease

Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease

Effects of Ecballium elaterium on brain in a rat model of sepsis-associated encephalopathy

Effects of Dapagliflozin on Experimental Sepsis Model in Rats

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