The effect of prolactin concentration on the efficacy of storing isolated porcine kidneys in a modified Biolasol solution

Abstract

Introduction: Biolasol is a solution developed in Poland for flushing the kidneys, liver, heart and pancreas by simple hypothermia method prior to transplantation. The solution supports the cellular integrity of grafts during ischemia-reperfusion injury (IRI).The aim of the study was to evaluate the impact of the concentration of prolactin added to Biolasol on selected biochemical parameters of kidney injury.Materials and methods: Biolasol was modified by the addition of prolactin at 1 μg/L, 10 μg/L and 100 μg/L and by ascorbicacid at 0.5 mmol/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase enzymes, sodium and potassium concentrations, pH and osmolarity parameters were assessed in the perfusates.Results: The addition of prolactin to Biolasol significantly improves the biochemical parameters of grafts in the models of rinsing, perfusion and reperfusion of isolated porcine kidneys.Conclusions: The study indicates the nephroprotective role of Biolasol with the addition of vitamin C and prolactin at a 100 μg/L.

Effect of Copper on the Function of Isolated Porcine Kidneys Stored Using Simple Hypothermia

“…We also conducted analyses of the efficacy of copper in the presence of prolactin (PRL) added to Biolasol fluid. The results of our previous studies suggest an effective action of PRL in protecting the kidney during ischemia [ 22 , 23 ].…”