The Effect of Proteolytic Enzyme Serratiopeptidase in the Treatment of Experimental Implant-Related Infection

Mecikoglu, Mete; Saygı, Baransel; Yıldırım, Yakup; Karadağ-Saygı, Evrim; Ramadan, Saleem Z.; Esemenli, Tanıl

doi:10.2106/jbjs.e.00007

Cited by 48 publications

(22 citation statements)

References 20 publications

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“…The emergence of antibiotic-resistant bacteria has led investigators to attempt to develop antibiotic-independent mechanisms to impair bacterial ability to form biofilm. 17,18 In S. epidermidis modulation of icaADBC expression, which promotes PIA-mediated biofilm, represents an attractive therapeutic target in this context.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide induced repression of icaADBC transcription and biofilm development in Staphylococcus epidermidis

Glynn

O’Donnell

Molony

et al. 2008

Journal Orthopaedic Research

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Expression of the icaADBC-encoded polysaccharide intercellular adhesion by Staphylococcus epidermidis promotes biofilm formation and represents an important virulence factor in biomaterial-related infections following orthopedic surgery. Biofilm development by the pathogen can be viewed as a protective reaction to environmental stressors including osmotic stress, thermal stress, and antimicrobial chemotherapy. Oxidative stress, arising from the release of toxic oxygen radicals by polymorphonuclear cells, is encountered by bacteria entering the body perioperatively. Evasion of this and other cell-mediated immune responses by pathogenic bacteria plays an important role in the development of chronic biomaterial-related infection. Here we investigated the impact of sublethal oxidative stress induced by H 2 O 2 (<18 mM) on S. epidermidis biofilm formation. S. epidermidis grown in brain heart infusion (BHI) media supplemented with 5 mM H 2 O 2 or 10 mM H 2 O 2 formed significantly less biofilm (p < 0.01 and p < 0.001, respectively) than bacteria grown in BHI alone. Consistent with this, using reverse transcription-polymerase chain reaction expression of the ica locus was also shown to be reduced by subinhibitory concentrations of H 2 O 2 . Furthermore, diminished ica operon expression correlated with increased expression of icaR, which encodes a repressor of icaADBC. Thus, these data suggest that mild oxidative stress downregulates biofilm development by S. epidermidis and may have potential in a therapeutic context. ß

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Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide induced repression of icaADBC transcription and biofilm development in Staphylococcus epidermidis

Glynn

O’Donnell

Molony

et al. 2008

Journal Orthopaedic Research

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“…Kokai-Kun et al (2009) showed that lysostaphin administered at 15 mg/kg in combination with 50 mg/kg nafcillin, three times per day for four days in mice, eradicated S. aureus biofilms from implanted catheters and eliminated heart and liver infections in S. aureus-infected mice (Kokai-Kun et al 2009). Mecikoglu et al (2006) showed that serratiopeptidase was effective for eradicating infection caused by biofilm-forming bacteria, and also that the anti-biofilm property of the enzyme may enhance antibiotic efficacy in the treatment of staphylococcal infections (Mecikoglu et al 2006). The different results found in the present study could be due to the type of enzyme.…”

Section: Discussionmentioning

confidence: 64%

“…The biofilm matrix is a collection of polysaccharides, proteins, nucleic acids, glycoproteins and phospholipids (Czaczyk and Myszka 2007). With this in mind, it is clear that enzymes can be used for degradation of biofilm (Johansen et al 1997;Berg et al 2001;Mecikoglu et al 2006;Lu and Collins 2007;Chaignon et al 2007). Enzymatic activity can be directed to the biofilm matrix, allowing better penetration of the subsequent antimicrobial agents used or can be targeted towards pathogen cell wall components in order to cause their lysis (Johansen et al 1997;Kaplan et al 2004).…”

Section: Original Papermentioning

confidence: 99%

Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows

Khoramian¹,

Emaneini²,

Bolourchi³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The objective of this study was to evaluate a combined antibiotic-enzyme therapy for Staphylococcus aureus mastitis and biofilm formation. A total of 141 cases of S. aureus chronic mastitis from three farms were divided into two groups: the control group (n = 54) were treated with Nafpenzal ® ointment; the enzyme + antibiotic group (n = 87) were treated with Nafpenzal ® plus an enzymatic ointment (MastiVeyxym ® ). Quantitative determination of biofilm formation was determined using a colorimetric microplate assay and the detection of ica genes by PCR. Enzyme + antibiotic therapy did not significantly improve cure rates compared to control (48.3 vs 38.9%). The cure rate for infections caused by biofilm-positive strains was 42.6 and 46.6% for control and enzyme + antibiotic groups, respectively (P > 0.05). Comparison of cure rates between farms showed a relationship with somatic cell count (SCC), parity and oxacillin resistance. 79.4% of the isolates produced biofilm and antibacterial resistance rates for oxacillin, penicillin and streptomycin were 25.5, 71.6 and 95.7%, respectively. These results indicate that while the increase in mastitis cure rate using enzymatic therapy was not significant, this treatment could be useful in some situations.

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“…Thus, serratiopeptidase has anti-inflammatory, analgesic, anti-edemic, antipathogenic, fibrinolytic and caseinolytic properties. In contradiction, Joshi and Nerurkar et al [27] conducted a study on rats (paw model) to evaluate the efficacy of serratiopeptidase in reducing edema. They concluded that serratiopeptidase was not an effective anti-inflammatory agent either alone or with diclofenac and further studies had to be done in humans.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

The Emerging Role of Serratiopeptidase in Oral Surgery: Literature Update

Kumar

2018

Asian J Pharm Clin Res

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Serratiopeptidase, a proteolytic enzyme derived from Serratia E-15 species enterobacteria, is widely used in medical field for its anti-inflammatory, anti-edemic properties, and analgesic properties. It is being used commonly in various specialties such as orthopedics, otolaryngology, gynecology, surgery, pulmonology, ophthalmology, and dentistry. Research has shown that serratiopeptidase is the most effective anti-inflammatory agent compared to other enzyme preparations. This article reviews the efficacy, safety, and applications of serratiopeptidase in oral surgery. This article also discusses the mechanism of action of serratiopeptidase, its contraindications and complications. From the recently published literature, it is clear that the role of serratiopeptidase as a therapeutic agent in oral and maxillofacial surgery is expanding and they hold a promising future as a broad-spectrum anti-inflammatory drug with minimal side effects and complications. Further, research will broaden their applications in the field of medicine and dentistry.

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The Effect of Proteolytic Enzyme Serratiopeptidase in the Treatment of Experimental Implant-Related Infection

Abstract: Serratiopeptidase was effective for eradicating infection caused by biofilm-forming bacteria in this experimental animal model. The antibiofilm property of the enzyme may enhance antibiotic efficacy in the treatment of staphylococcal infections.

Cited by 48 publications

References 20 publications

Hydrogen peroxide induced repression of icaADBC transcription and biofilm development in Staphylococcus epidermidis

Hydrogen peroxide induced repression of icaADBC transcription and biofilm development in Staphylococcus epidermidis

Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows

The Emerging Role of Serratiopeptidase in Oral Surgery: Literature Update

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