The effect of Pseudomonas chlororaphis subsp. aurantiaca strain Q16 able to inhibit Fusarium oxysporum growth on potato yield

Poštić, Dobrivoj; Jošić, Dragana; Lepsanovic, Zorica; Aleksić, Goran; Latković, Dragana; Starović, Mira

doi:10.5937/ratpov56-20428

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…stra ins aga inst dif fe rent phyto pat ho ge nic fun gi we re re por ted by Dju ric et al (2011), andJo šić et al (2015). P. chlo ro rap his stra ins har bo u ring mul ti ple PGP tra its, in clu ding an ti bi o tics phe na zi ne-1-car boxylic acid (PCA) and 2-hydroxy-phena zi ne-1-car boxylic acid (2-OH-PCA), suc cessfully in hi bi ted Al ter na ria al ter na ta growth and di se a se in ci den ce on car doon (Jo šić et al, 2012), P. the i co la, and F. oxyspo rum (Sta ro vić et al, 2017;Poš tić et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.

Starović

Ristić

Dj.

et al. 2021

Zb Mat srp prir nauk

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Leaf spot disease caused by Cercospora beticola Sacc. is the most destructive foliar disease of beet. Cercospora leaf spot is controlled primarily by fungicides because the non-chemical alternatives do not provide commercially viable control. One of the ways of reducing chemical application is the use of different essential oils (EOs) or antagonistic plant growth-promoting rhizobacteria (PGPB). This study evaluates several EOs and PGPB belonging to Pseudomonas chlororaphis as possible control agents of this pathogen. Antifungal properties were determined by in vitro microdilution method against five C. beticola monosporial isolates originated from the locality Brus, Serbia (53?53? N, 21?04?E and 429 m above sea level) using EOs from medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum), and myrtle (Myrtus communis) obtained by a hydro-distillation method. All tested oils displayed some antifungal activity against the fungal isolates. Origanum vulgare EO demonstrated the strongest antifungal activity (MIC - 0.0055?0.0051mg/mL), Ocimum basilicum slightly lower (MIC - 0.075?0.045mg/mL), followed by Myrtus communis (MIC - 0.775?0.045 mg/ mL) and Echinophora tenuifolia (MIC - 7.75?4.5 mg/mL). Five tested P. chlororaphis strains exhibited some antagonistic effect against C. beticola. Overnight culture (ONC) of P. chlororaphis strain E65 induced the highest percentage of inhibition (75.8%), followed by N3 (72.0%). A cell-free supernatant (CFS) and the CFS treated with EDTA (CFS-EDTA) of these strains showed similar inhibition of 60.2 and 56.0%, and both strains suppressed C. beticola growth. P. chlororaphis strains M1 and K113 also reduced the fungal growth by 67-70% using ONC and between 48-57% using different CFS fractions. The strains L1 and B25 caused inhibition of 60% using ONC and 50% by CFS. The lowest inhibition (~40%) by CFS-EDTA and heat-treated cell-free supernatant (HT-CFS) was recorded for B25, which was used as a reference strain. The tested isolates of C. beticola were susceptible to all selected essential oils and P. chlororaphis strains E25, N3, M1, and K113 in vitro, making them a promising non-chemical control agent. It is recommended that these findings should be tested in field conditions.

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Section: Introductionmentioning

confidence: 99%

Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.

Starović

Ristić

Dj.

et al. 2021

Zb Mat srp prir nauk

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Synergistic interaction between Azotobacter and Pseudomonas bacteria in a growth-stimulating consortium

Serazetdinova,

Chekushkina,

Borodina

et al. 2024

Foods and Raw Materials

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Intensifying agricultural production involves an active use of agrochemicals, which results in disrupted ecological balance and poor product quality. To address this issue, we need to introduce biologized science-intensive technologies. Bacteria belonging to the genera Azotobacter and Pseudomonas have complex growth-stimulating properties and therefore can be used as a bioproduct to increase plant productivity. We aimed to create a growth-stimulating consortium based on the strains of the genera Azotobacter and Pseudomonas, as well as to select optimal cultivation parameters that provide the best synergistic effect. We studied strains Azotobacter chroococcum B-4148, Azotobacter vinelandii B-932, and Pseudomonas chlororaphis subsp. aurantiaca B-548, which were obtained from the National Bioresource Center “All-Russian Collection of Industrial Microorganisms” of Kurchatov Institute. All the test strains solubilized phosphates and produced ACC deaminase. They synthesized 0.98–1.33 mg/mL of gibberellic acid and produced 37.95–49.55% of siderophores. Their nitrogen-fixing capacity ranged from 49.23 to 151.22 μg/mL. The strain had high antagonistic activity against phytopathogens. In particular, A. chroococcum B-4148 and A. vinelandii B-932 inhibited the growth of Fusarium graminearum, Bipolaris sorokiniana, and Erwinia rhapontici, while P. chlororaphis subsp. aurantiaca B-548 exhibited antagonism against F. graminearum and B. sorokiniana. Since all the test strains were biologically compatible, they were used to create several consortia. The greatest synergistic effect was achieved by Consortium No. 6 that contained the strains B-4148, B-932, and B-548 in a ratio of 1:3:1. The optimal nutrient medium for this consortium contained 25.0 g/L of Luria-Bertani medium, 8.0 g/L molasses, 0.1 g/L magnesium sulfate heptahydrate, and 0.01 g/L of aqueous manganese sulfate. The optimal cultivation temperature was 28°C. The microbial consortium created in our study has high potential for application in agricultural practice. Further research will focus on its effect on the growth and development of plants, in particular cereal crops, under in vitro conditions and in field experiments.

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The effect of Pseudomonas chlororaphis subsp. aurantiaca strain Q16 able to inhibit Fusarium oxysporum growth on potato yield

Cited by 2 publications

References 27 publications

Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.

Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.

Synergistic interaction between Azotobacter and Pseudomonas bacteria in a growth-stimulating consortium

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