This study highlights the theme of human error of train drivers, conducting empirical analysis on the relationship between organizational culture, safety climates, safety behavior, and accident. The empirical test results based on questionnaires received from 223 train drivers working at A subway firm indicated that relationship conflict and psychological reward as the elements of organizational culture variables showed a significant positive effect on CEO philosophy, communication, and boss's leadership. And only boss's leadership showed a positive influence on safety behavior, which in turn showed a significant negative relationship with accidents.