The Effect of Purified Human Bone Marrow–Derived Mesenchymal Stem Cells on Rotator Cuff Tendon Healing in an Athymic Rat

Degen, Ryan M.; Carbone, Andrew; Carballo, Camila; Zong, Jingfeng; Chen, Tony; Lebaschi, Amir; Liang, Ying; Deng, Xiang‐Hua; Rodeo, Scott A.

doi:10.1016/j.arthro.2016.04.019

Cited by 48 publications

(66 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ultimate load to failure was 19.0 ± 4.5 N in control and 24.2 ± 8.0 N in tHG group at 8 weeks after repair . For the current study, an increase in strength of 20% would be considered clinically significant . Using these estimations, a power of 0.80 was achieved using 14 specimens per group with α = 0.05 for biomechanical testing.…”

Section: Methodsmentioning

confidence: 95%

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Kaizawa

Franklin

Leyden

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

Rotator cuff (RTC) repair outcomes are unsatisfactory due to the poor healing capacity of the tendon bone interface (TBI). In our preceding study, tendon hydrogel (tHG), which is a type I collagen rich gel derived from human tendons, improved biomechanical properties of the TBI in a rat chronic RTC injury model. Here we investigated whether adipose‐derived stem cell (ASC)‐seeded tHG injection at the repair site would further improve RTC healing. Rats underwent bilateral supraspinatus tendon detachment. Eight weeks later injured supraspinatus tendons were repaired with one of four treatments. In the control group, standard transosseous suture repair was performed. In the ASC, tHG, tHGASC groups, ASC in media, tHG, and ASC‐seeded tHG were injected at repair site after transosseous suture repair, respectively. Eight weeks after repair, the TBI was evaluated biomechanically, histologically, and via micro CT. Implanted ASCs were detected in ASC and tHGASC groups 7 weeks after implantation. ACS implantation improved bone morphometry at the supraspinatus insertion on the humerus. Injection of tHG improved biomechanical properties of the repaired TBI. RTC healing in tHGASC group was significantly better than control but statistically equivalent to the tHG group based on biomechanical properties, fibrocartilage area at the TBI, and bone morphometry at the supraspinatus insertion. In a rat RTC chronic injury model, no biomechanical advantage was gained with ASC augmentation of tHG. Clinical Significance: Tendon hydrogel augmentation with adipose derived stem cells does not significantly improve TBI healing over tHG alone in a chronic rotator cuff injury model. © 2019 Orthopaedic Research Society. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

show abstract

Section: Methodsmentioning

confidence: 95%

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Kaizawa

Franklin

Leyden

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…They showed that SCX led to a significance increase in the strength of repair and the amount of fibrocartilage at 4 weeks. Recently, human BM‐MSCs have been transplanted in an athymic rat rotator cuff tendon repair model . Fibrocartilage formation, collagen orientation, and biomechanical strength were improved 2 weeks after transplantation.…”

Section: A Review Of Current Cell‐based Approaches In Soft Tissue Repmentioning

confidence: 99%

Tissue‐specific endothelial cells: a promising approach for augmentation of soft tissue repair in orthopedics

Lebaschi

Nakagawa

Wada

et al. 2017

Annals of the New York Academy of Sciences

Self Cite

View full text Add to dashboard Cite

Biologics are playing an increasingly significant role in the practice of modern medicine and surgery in general and orthopedics in particular. Cell-based approaches are among the most important and widely used modalities in orthopedic biologics, with mesenchymal stem cells and other multi/pluripotent cells undergoing evaluation in numerous preclinical and clinical studies. On the other hand, fully differentiated endothelial cells (ECs) have been found to perform critical roles in homeostasis of visceral tissues through production of an adaptive panel of so-called "angiocrine factors." This newly discovered function of ECs renders them excellent candidates for novel approaches in cell-based biologics. Here, we present a review of the role of ECs and angiocrine factors in some visceral tissues, followed by an overview of current cell-based approaches and a discussion of the potential applications of ECs in soft tissue repair.

show abstract

“…The results showed improved fibrocartilage formation, collagen orientation, and biomechanical strength 2 weeks following repair 65 . Further analysis of the data demonstrate that Indian hedgehog (Ihh) and Sox9 signaling play an important role in the tendon-to-bone healing mechanism 66 .…”

Section: Current Cell-based Approachesmentioning

confidence: 94%

Cell-based Approaches for Augmentation of Tendon Repair

Carballo

Lebaschi

Rodeo

2017

Techniques in Shoulder &Amp; Elbow Surgery

Self Cite

View full text Add to dashboard Cite

Cell-based approaches are among the principal interventions in orthobiologics to improve tendon and ligament healing and to combat degenerative processes. The number of options available for investigation are expanding rapidly and investigators have an increasing number of cell types to choose from for research purposes. However, in part due to the current regulatory environment, the list of available cells at clinicians’ disposal for therapeutic purposes is still rather limited. In this review, we present an overview of the main cellular categories in current use. Notable recent developments in cell-based approaches include the introduction of diverse sources of mesenchymal stem cells, pluripotent cells of extra-embryonic origin, and the emerging popularity of fully differentiated cells such as tenocytes and endothelial cells. Delivery strategies are discussed and a succinct discussion of the current regulatory environment in the United States is presented.

show abstract

The Effect of Purified Human Bone Marrow–Derived Mesenchymal Stem Cells on Rotator Cuff Tendon Healing in an Athymic Rat

Cited by 48 publications

References 37 publications

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Tissue‐specific endothelial cells: a promising approach for augmentation of soft tissue repair in orthopedics

Cell-based Approaches for Augmentation of Tendon Repair

Contact Info

Product

Resources

About