The Effect of Reactive Oxygen Species on Respiratory Complex I Activity in Liposomes
Jana Eisermann,
Yuxin Liang,
John J. Wright
et al.
Abstract:Respiratory complex I (R‐CI) is an essential enzyme in the mitochondrial electron transport chain but also a major source of reactive oxygen species (ROS), which are implicated in neurodegenerative diseases and ageing. While the mechanism of ROS production by R‐CI is well‐established, the feedback of ROS on R‐CI activity is poorly understood. Here, we perform EPR spectroscopy on R‐CI incorporated in artificial membrane vesicles to reveal that ROS (particularly hydroxyl radicals) reduce R‐CI activity by making … Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.