The effect of recryopreservation on embryo viability and outcomes of in vitro fertilization: a systematic review and meta-analysis

Wang, Xiangfei; Mao, Ruolin; Wang, Meng; Li, Rui; Jin, Liu; Zhu, Liping

doi:10.1016/j.fertnstert.2023.03.001

Cited by 12 publications

(3 citation statements)

References 44 publications

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“…This is in line with the results of Zheng et al (2017), which found a 10% reduction (p<.05) in LBR of double cryopreserved embryos (slow freezing followed by vitrification), despite the non-significant difference in CPR. Moreover, the studies of Wang et al (2023) and Li et al (2021) also found reduced LBR for double-vitrified embryos, while other published studies indicated no significant difference in LBR between double-and single-cryopreserved embryos.…”

Section: Discussionmentioning

confidence: 86%

“…Biochemical pregnancy rate, miscarriage rate and birthweight were also analyzed in the present study, but no significant differences were found between single-and double-vitrified embryos. Apart from Zheng et al (2017) and Wang et al (2023), who found significantly higher rates of miscarriage in double cryopreserved embryos, the rest of the studies indicated no effect in either miscarriage rate or birthweight (Kumasako et al, 2009;Koch et al, 2011;Murakami et al, 2011;Hallamaa et al, 2021;Shen et al, 2023).…”

Section: Discussionmentioning

confidence: 96%

“…Additionally, Farhi et al concluded that CPR was significantly reduced (28.2% reduction, p<.05) in re-cryopreserved embryo cycles, but the study applied slow freezing in both cryopreservation steps. Moreover, the only meta-analyses published to date concerning the effect of embryo re-cryopreservation on clinical outcomes concluded that double cryopreservation led to impaired IVF success rates (Wang et al, 2023). However, Kumasako et al (2009), in a study using vitrification only, reported no adverse effect of double vitrification in CPR.…”

Section: Discussionmentioning

confidence: 99%

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Double vitrification of embryos adversely affects clinical outcomes

Oraiopoulou,

Karagianni,

Papatheodorou

et al. 2024

JBRA

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Objective: To evaluate the impact of double embryo vitrification on clinical outcomes.Methods: This retrospective cohort study included data from January 2013 to March 2021. The study group included women aged 33.3±5.7 years with double-vitrified embryos (n=381), while the control group included women aged 32.1±6.7 years with embryos vitrified once (n=780), all transferred at the blastocyst stage. The primary endpoint was live birth rate (LBR), and secondary endpoints included percent positive βHCG test, clinical/ongoing pregnancy rates, miscarriage/biochemical pregnancy rates and birthweight.Results: LBR was significantly lower in double-vitrified embryos (30.2%) than in embryos vitrified once (45.6%, p<.05). Similarly, double-vitrified embryos were associated with significantly lower positive βHCG tests (46% vs. 63.3%, p<.05) and clinical (34.9% vs. 52.2%, p<.05) and ongoing pregnancy (31.3% vs. 47.3%, p<.05) rates compared to embryos vitrified once. However, biochemical pregnancy (double vitrified: 24.1% vs. vitrified once: 17.9%, p>.05) and miscarriage rates (double vitrified: 10.2% vs. vitrified once: 9.4%, p>.05), as well as mean birthweight (double-vitrified embryos: 2950g vs. embryos vitrified once: 2837g, p>.05) did not differ significantly between two groups. On a secondary comparison, amongst double-vitrified embryos, the subgroup that was cultured for more than 24 hours between warming and second vitrification achieved significantly higher positive βHCG tests (49%) and clinical pregnancy (38%) rates, compared to embryos re-vitrified on the same day of warming (31.8% and 20.5%, respectively, p<.05). Nevertheless, LBR did not differ significantly amongst these study-group embryos (embryos that remained in culture for more than 24 hours: 32.2% vs. embryos that were re-vitrified on warming day: 20.5%, p>.05).Conclusions: Double vitrification of embryos adversely affects clinical outcomes. However, it represents a valuable option concerning embryo wastage, with acceptable success rates.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Double vitrification of embryos adversely affects clinical outcomes

Oraiopoulou,

Karagianni,

Papatheodorou

et al. 2024

JBRA

View full text Add to dashboard Cite

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Effect of re‐cryopreservation on pregnancy and neonatal outcomes: Analysis using propensity score matching

Xue

Shi

2023

Intl J Gynecology & Obste

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ObjectiveTo comprehensively assess the effect of re‐cryopreservation procedure on pregnancy and neonatal outcomes by using propensity matching (PSM) analysis.MethodsThe patients were divided into two groups according to the times of embryo cryopreservation: the cryopreservation group (n = 8034) and the re‐cryopreservation group (n = 66). To optimize the precision of the present study, we used PSM to adjust the different baseline characteristics between the two groups, including maternal age, the number of good‐quality embryos transferred and endometrial preparation protocols. The primary outcome was live‐birth rate. The secondary outcomes were biochemical pregnancy rate, clinical pregnancy rate, and miscarriage rate.ResultsWe found that the rates of biochemical pregnancy and clinical pregnancy were comparable between the two groups, whereas miscarriage rate was increased, resulting in significantly reduced live‐birth rate in the re‐cryopreservation group. No differences were observed in terms of neonatal outcomes, including cesarean section, birth weight, and malformation as well as pregnancy complications. Moreover, multivariable analysis demonstrated that re‐cryopreservation was an independent risk factor for live‐birth rate.ConclusionThe present study demonstrated the adverse effect of re‐cryopreservation on pregnancy outcomes, providing valuable information for clinical decision making and patient counseling.

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Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis

Wang,

Zhou,

Long

et al. 2024

MedComm

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Recryopreservation (recryo) is occasionally applied in clinical, while the underlying mechanism of impaired clinical outcomes after recryo remains unclear. In this study, frozen embryo transfer (FET) cycles of single blastocyst transfer in an academic reproductive medicine center were enrolled. According to the number of times blastocysts experienced cryopreservation, they were divided into the cryopreservation (Cryo) group and the Recryo group. Donated human blastocysts were collected and detected for mechanism exploration. It was found that recryo procedure resulted in impaired blastocyst developmental potential, including decreased implantation rate, reduced biochemical pregnancy rate, declined clinical pregnancy rate, higher early miscarriage rate, and lower live birth rate. Moreover, recryo led to impaired trophectoderm (TE) function, exhibiting lower human chorionic gonadotropin levels 12 days after FET. In addition, single‐cell RNA sequencing showed that the expression of genes involved in cell adhesion and embryo development were altered. More specifically, activated endoplasmic reticulum (ER) pathway and induced apoptosis were further verified by immunofluorescence and terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) assay involving in the recryo procedure. In conclusion, recryo could interfere with the process of blastocyst implantation by impairing TE function, affecting blastocyst adhesion, activating ER stress pathway and inducing apoptosis. It provides caution to embryologists about the potential risk of recryopreservation.

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The effect of recryopreservation on embryo viability and outcomes of in vitro fertilization: a systematic review and meta-analysis

Cited by 12 publications

References 44 publications

Double vitrification of embryos adversely affects clinical outcomes

Double vitrification of embryos adversely affects clinical outcomes

Effect of re‐cryopreservation on pregnancy and neonatal outcomes: Analysis using propensity score matching

Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis

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