The effect of repetitive stimulation at low frequencies upon the electrical and mechanical activity of single muscle fibres

Grabowski, Władysław; Ea, Lobsiger; Hc, Lüttgau

doi:10.1007/bf00626225

Cited by 93 publications

(43 citation statements)

References 20 publications

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“…In our previous paper (KANAYA et al, 1980), the resting and action potentials in fatigued muscle fibers were examined in detail and the results obtained were in agreement with that of previous investigators (MASHIMA et al, 1962;EBERSTEIN and SANDOW 1963;GRABOWSKI et al, 1972). 1) Dependence of caffeine contracture on caffeine concentrations in fatigued muscle fibers Relation between the peak tension of caffeine contractures and the caffeine concentrations (1 to 10 mM) (dose-response curve) was examined in normal muscle fibers and in the fatigued muscle fibers in which twitch tension had been almost completely inhibited by repetitive stimulations at 2 Hz.…”

Section: Resultssupporting

confidence: 88%

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Properties of caffeine- and potassium-contractures in fatigued frog single twitch muscle fibers.

Kanaya¹,

Takauji²,

Nagai³

1983

Jpn.J.Physiol

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The properties of caffeine contracture and potassium contracture in fatigued single fibers were examined in detail using frog semitendinosus muscle, Rana japonica. Fatigue was caused by repetitive stimulation at 2 Hz. The dose-response curve of caffeine contracture in the fatigued fibers was shifted toward the right; the threshold concentration of caffeine for the contracture in normal fibers was 1.5 mM, whereas that in fatigued fibers was 5 mM. However, in the presence of 25 mM K+ or 0.01 % Triton X-100, caffeine contractures occurred sufficiently at the lower concentrations (3-5 mM) even in the fatigued fibers. Furthermore, in the fatigued fibers, the peak tension of the initial component of biphasic potassium contracture with 60 or 80 mM KCl (Cl constant; 120 mM) was slightly inhibited, whereas the secondary component of the contracture was markedly inhibited. These results indicate that the permeability to caffeine of the transverse tubular membrane (T-membrane) of the fibers and the Ca influx in response to the direct depolarization of T-membrane with K+ are markedly inhibited in the fatigued fibers.

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Section: Resultssupporting

confidence: 88%

“…It is generally accepted that the fatigue in skeletal muscle fibers could be the result of an inhibition of excitation-contraction coupling (E-C coupling) (MASHIMA et a!., 1962;EBERSTEIN and SANDOW, 1963;GRABOWSKI et al, 1972;KANAYA et al, 1980). However, the detailed mechanism and the sites of the inhibition have not yet been clarified.…”

mentioning

confidence: 99%

Properties of caffeine- and potassium-contractures in fatigued frog single twitch muscle fibers.

Kanaya¹,

Takauji²,

Nagai³

1983

Jpn.J.Physiol

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“…Está demostrado que la caída del potencial de acción no es la causa de la fatiga (92)(93)(94), tampoco el agotamiento del ATP o de las reservas energéticas (92,95), aunque se ha planteado que una caída localizada de ATP cercana a puntos críticos podría ser importante (88). En lo que respecta a la alteración del fenómeno del acoplamiento excitación-contracción, la investigación se ha centrado en los fenómenos de regulación de la liberación y recaptura de Ca 2+ (52).…”

Section: Mecanismos Central Y Periférico De La Fatiga Muscularunclassified

El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

Calderón-Vélez

Figueroa

2009

biomedica

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El mecanismo de acoplamiento excitación-contracción fue definido en el músculo esquelético como la secuencia de eventos que ocurre desde la generación del potencial de acción en la fibra muscular hasta que se inicia la generación de tensión. La regulación e interacción de dichos eventos entre sí ha sido estudiada durante los últimos 50 años utilizando diferentes técnicas, con las cuales se estableció la importancia y origen del ion calcio como activador contráctil, se conocen las principales proteínas involucradas y se inició el estudio de la base ultraestructural y de la regulación farmacológica; además, hay evidencias de que el acoplamiento excitación-contracción se altera en diferentes situaciones como en el envejecimiento, en la fatiga muscular y en algunas enfermedades musculares. Sin embargo, aún hay varias preguntas por responder: ¿cómo es el desarrollo y envejecimiento del mecanismo de acoplamiento excitación-contracción?, ¿cuál es su papel en la fatiga muscular y en algunas enfermedades musculares?, ¿cuál es la naturaleza de la interacción entre diferentes proteínas involucradas en el acoplamiento excitación-contracción? La presente revisión describe el acoplamiento excitación-contracción en el músculo esquelético y las técnicas utilizadas para su estudio como introducción para discutir algunas de las preguntas que aún falta por responder al respecto.Palabras clave: músculo esquelético, contracción muscular, relajación muscular, calcio, canal liberador de calcio, canales de calcio tipo L, receptor de rianodina, fatiga muscular. Excitation-contraction coupling in skeletal muscle: questions remaining after 50 years of researchThe excitation-contraction coupling mechanism was defined as the entire sequence of reactions linking excitation of plasma membrane to activation of contraction in skeletal muscle. By using different techniques, their regulation and interactions have been studied during the last 50 years, defining until now the importance and origin of the calcium ion as a contractile activator and the main proteins involved in the whole mechanism. Furthermore, the study of the ultrastructural basis and pharmacological regulation of the excitation-contraction coupling phenomenon has begun. The excitation-contraction coupling is thought to be altered in situations as ageing, muscle fatigue and some muscle diseases. However, many questions remain to be answered. For example, (1) How excitation-contraction coupling develops and ages? (2) What role does it play in muscle fatigue and other diseases? (3) What is the nature of the interaction between the proteins believed to be involved? The present review describes excitation-contraction coupling in skeletal muscle and techniques used to better understand it as an introduction for discussing unanswered questions regarding excitation-contraction coupling.

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“…The potentiated twitch tension was never sustained at the maximum steady level, instead, it declined gradually (not shown). This indicates that the stage of fatigue was reached as MASHIMA et al (1962) andGRABOWSKI et al (1972) have observed. Figure 1B, from another muscle, shows the dependency of the staircase on the stimulation frequency.…”

Section: Twitch Staircasementioning

confidence: 55%

“…The present finding that the effects of caffeine in low concentration were similar to those of low temperature would suggest the possibility of an increase in the sarcoplasmic calcium level which consequently affects the intracellular calcium turnover process. The impairment of the contractile protein system after twitch train is unlikely because GRABOWSKI et al (1972) showed that maximum contracture tension could be produced even in the fatigued muscle, when muscle membrane is sufficiently depolarized by potassium solution which contained caffeine. The machanism for the after-potentiation is not clear.…”

Section: Effects Of Twitch On Tetanus 445mentioning

confidence: 99%

Effects of twitch train on the tetanic contractility of the frog skeletal muscle.

Kawata

1983

Jpn.J.Physiol

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The effects of twitch trains on the contractility of succeeding tetani were investigated in the frog toe muscle. The changes in isometric tension and its first derivative were analyzed. Tetani of 1 sec duration were induced every 5 min and trains of twitches (less than 250) with 0.1 to 3 Hz were interposed between two successive tetani. A twitch train which clearly shows an ascending staircase exerted at least three different effects on the following tetani. These were a rapidly decaying potentiation (P1), a slowly decaying potentiation (P2), and an inhibitory effect (1) which was regarded as fatigue. These after-effects were modified by various interventions. Increasing of the twitch frequency at a constant number of stimuli augmented both the potentiating and the inhibiting effects. When the bath temperature was lowered to 4°C, the potentiation was masked by a marked inhibition. At a higher temperature (28°C) the potentiating effect was facilitated. Prolonged perfusion of low concentration (0.5 mM) of caffeine mimicked the effects of low temperature. Effects of twitch train on the contracture induced by 5 mM caffeine were examined and it was found that the repetitive twitches had no effect on the following caffeine contracture. Although the exact mechanism for these after-effects was not clear, it was assumed that the intracellular calcium turnover may play some role in the observed phenomena.

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The effect of repetitive stimulation at low frequencies upon the electrical and mechanical activity of single muscle fibres

Cited by 93 publications

References 20 publications

Properties of caffeine- and potassium-contractures in fatigued frog single twitch muscle fibers.

Properties of caffeine- and potassium-contractures in fatigued frog single twitch muscle fibers.

El acoplamiento excitación-contracción en el músculo esquelético: preguntas por responder a pesar de 50 años de estudio

Effects of twitch train on the tetanic contractility of the frog skeletal muscle.

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