The effect of reserpine, oestradiol and in-vitro perfusion on oviduct calcium levels in the mouse during egg transport

Abstract

Summary. Mouse

Investigation of the influence of progesterone on mouse embryo transport by using antiprogestational steroids

“…Albino mice of the RGIT/SLAC strain were housed as described previously (Lee & Kendle, 1979 (Welch, 1947 The mean numbers of embryos recovered were within the range 9-5 ± 3-7 to 14-1 + 1-0 with no significant differences between groups (multiple range / test). (Kendle, 1978) …”

Method for maintaining embryo transport in mouse oviducts in vitro

“…In particular, the role of the 50% fall in tissue calcium levels which occurs in vivo at the time of embryo passage through the ampullary-isthmic junction (Lee & Kendle, 1979) will be investigated by altering the concentration of calcium in the incubation medium.…”

“…Kendle (1969) examined incubated oviducts from mice killed on Day 1 of pregnancy for periods of up to 48 h. Using Ringer solution or culture media, he found no net forward movement of embryos in vitro. Similarly Lee (1979) and Lee & Kendle (1979) reported a technique for the continuous monitoring of embryo position and visual assessment of tubai contractility in vitro for periods of up to 20 h from mice killed on Day 2 of pregnancy, and although finding peristaltic/antiperistaltic contractions throughout this period, no net forward movement of embryos was seen, regardless of the experimental or pharmacological manipulation carried out. Talo (1980) studied electrophysiological activity in the mouse oviduct in vitro with ova in situ.…”