The Effect of Resveratrol in the Proliferation and Autophagy of Breast Cancer Cell Lines

Abstract

Breast cancer (BC) is one of the most universal malignant tumors of worldwide in women and it is a significant public health problem in terms of both morbidity and mortality. BC is the second leading cause of death among American women. It is estimated 250,000 new cases of invasive BC will be diagnosed in women in 2017. For instance, there still existing the need for developing or identify new effective compounds to fight this disease. For example resveratrol, which is a plant derived polyphenol with anti‐estrogenic and antioxidant properties. In‐vitro studies indicated resveratrol has anticancer properties such as cell cycle arrest, apoptosis induction, prevention of DNA damage, and angiogenesis inhibition. However, scientific literature studying the effect of resveratrol on DNA synthesis and in autophagy is limited. For this reason we studied the effect of resveratrol in the proliferation of three breast cell lines CCD‐1074 (non‐malignant cells), CCD‐1097 (transitional cells), and MCF‐7 (malignant non‐invasive cells); and autophagy induction in the MCF‐7 cell line. We hypothesized that resveratrol will decrease cell proliferation and increase autophagy in BC cells when compared to control treatments. We studied the proliferation effect in cells treated with resveratrol at 25, 50, 100, 200 μM during 24 hours post treatment. We used Bromodeoxyuridine (BrdU) and the detection of translocation of LC3 via flow cytometry to study proliferation and autophagy, respectively. Our results showed a significant decrease in cell proliferation at resveratrol concentrations of 100 and 200 μM across all cell lines when compared to the vehicle control (p<0.05). Importantly, at 50 μM resveratrol affected only transitional and malignant cell lines without affecting our non‐malignant cell line. Induction of autophagy was significantly higher when MCF‐7 cells were treated with 100 μM and 200 μM compared to the vehicle control (p <0.05). However, we are currently in the process of evaluating the effects of resveratrol in other malignant and non‐malignant cell lines. We consider that the 50 μM concentration as a possible therapeutic window selectively to inhibit cell proliferation and induce autophagy.Support or Funding InformationDo not applyThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.